GNE-0723

Cat. No.: HY-108337: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

GNE-0723 is a brain permeable positive allosteric modulator of NMDAR, with an EC₅₀ of 21 nM for GluN2A, 7.4 and 6.2 μM for GluN2C and GluN2D, respectively.

For research use only. We do not sell to patients.

GNE-0723 Chemical Structure

CAS No. : 1883518-31-7

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Purity & Documentation
References
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Description

GNE-0723 is a brain permeable positive allosteric modulator of NMDAR, with an EC₅₀ of 21 nM for GluN2A, 7.4 and 6.2 μM for GluN2C and GluN2D, respectively^[1].

IC₅₀ & Target

EC50: 21 nM (GluN2A), 7.4 μM (GluN2C), 6.2 μM (GluN2D)^[1]

In Vitro

GNE-0723 is a brain permeable positive allosteric modulator of NMDAR, with an EC₅₀ of 21 nM for GluN2A, 7.4 and 6.2 μM for GluN2C and GluN2D, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

467.78

Formula

C₁₆H₈ClF₆N₅OS

CAS No.

1883518-31-7

Appearance

Solid

Color

White to yellow

SMILES

N#C[C@H]1[C@H](C2=C(C(F)(F)F)SC3=NC(CN4N=C(C(F)(F)F)C=C4Cl)=CC(N32)=O)C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 190 mg/mL (406.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1378 mL	10.6888 mL	21.3776 mL
5 mM	0.4276 mL	2.1378 mL	4.2755 mL
10 mM	0.2138 mL	1.0689 mL	2.1378 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 4.75 mg/mL (10.15 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 4.75 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (47.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 4.75 mg/mL (10.15 mM); Clear solution

This protocol yields a clear solution of ≥ 4.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (47.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 4.75 mg/mL (10.15 mM); Clear solution

This protocol yields a clear solution of ≥ 4.75 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (47.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.74% ee.: 99.9586%

Select Batch:

Data Sheet (273 KB) SDS (251 KB)

COA (250 KB) HNMR (510 KB) LCMS (267 KB)

Handling Instructions (2659 KB)

References

[1]. Villemure E, et al. GluN2A-Selective Pyridopyrimidinone Series of NMDAR Positive Allosteric Modulators with an Improved in Vivo Profile. ACS Med Chem Lett. 2016 Oct 31;8(1):84-89. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.1378 mL	10.6888 mL	21.3776 mL	53.4439 mL
	5 mM	0.4276 mL	2.1378 mL	4.2755 mL	10.6888 mL
	10 mM	0.2138 mL	1.0689 mL	2.1378 mL	5.3444 mL
	15 mM	0.1425 mL	0.7126 mL	1.4252 mL	3.5629 mL
	20 mM	0.1069 mL	0.5344 mL	1.0689 mL	2.6722 mL
	25 mM	0.0855 mL	0.4276 mL	0.8551 mL	2.1378 mL
	30 mM	0.0713 mL	0.3563 mL	0.7126 mL	1.7815 mL
	40 mM	0.0534 mL	0.2672 mL	0.5344 mL	1.3361 mL
	50 mM	0.0428 mL	0.2138 mL	0.4276 mL	1.0689 mL
	60 mM	0.0356 mL	0.1781 mL	0.3563 mL	0.8907 mL
	80 mM	0.0267 mL	0.1336 mL	0.2672 mL	0.6680 mL
	100 mM	0.0214 mL	0.1069 mL	0.2138 mL	0.5344 mL

GNE-0723 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GNE-07231883518-31-7GNE0723GNE 0723iGluRIonotropic glutamate receptorsInhibitorinhibitorinhibit

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