1. Stem Cell/Wnt
  2. Wnt
  3. IQ 1

IQ 1 

Cat. No.: HY-10593 Purity: 99.69%
Handling Instructions

IQ 1 is a Wnt/β-catenin/CBP signalling sustainer. IQ 1 maintains long-term expansion of Wnt/β-catenin-driven mouse embryonic stem cells (ESCs) and prevents spontaneous differentiation by enhancing β-catenin/CBP-mediated transcription and preventing conversion to β-catenin/p300-mediated transcription. IQ-1 regulates Wnt signalling by interacting with PR72/130. IQ 1 can be used in study of ESCs expansion.

For research use only. We do not sell to patients.

IQ 1 Chemical Structure

IQ 1 Chemical Structure

CAS No. : 331001-62-8

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 87 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 87 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 79 In-stock
Estimated Time of Arrival: December 31
10 mg USD 106 In-stock
Estimated Time of Arrival: December 31
25 mg USD 238 In-stock
Estimated Time of Arrival: December 31
50 mg USD 343 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Based on 1 publication(s) in Google Scholar

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Description

IQ 1 is a Wnt/β-catenin/CBP signalling sustainer. IQ 1 maintains long-term expansion of Wnt/β-catenin-driven mouse embryonic stem cells (ESCs) and prevents spontaneous differentiation by enhancing β-catenin/CBP-mediated transcription and preventing conversion to β-catenin/p300-mediated transcription. IQ-1 regulates Wnt signalling by interacting with PR72/130. IQ 1 can be used in study of ESCs expansion[1].

In Vitro

IQ-1 (1.10, 3.48, 11.04 mM; 7 days) maintains the undifferentiated state of ESCs[1].
IQ-1 (0.28, 1.10, 2.76, 11.04 mM; 21 h) maintains murine ESC self-renewal independently of LIF[1].
IQ-1 Binds the PR72/130 Subunit of the Serine/Threonine Phosphatase PP2A in P19 cells[1].
IQ-1 (10 µM; 24 h) modulates Wnt signaling via interaction with PR72/130 in P19 cells[1].
IQ-1 (10 µM; 24 h) indirectly decreases the phosphorylation of p300 Ser-89 and Thereby the β-Catenin/p300 interaction[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Embryonic stem cells (ESCs)
Concentration: 1.10, 3.48, 11.04 mM
Incubation Time: 7 days
Result: Dose dependently increased alkaline phosphatase activity, in media containing 15% FCS without the addition of exogenous leukemia inhibitory factor (LIF).
Maintained SSEA-1 (undifferentiated ESC marker) expression dose-dependently.

RT-PCR[1]

Cell Line: Embryonic stem cells (ESCs)
Concentration: 0.28, 1.10, 2.76, 11.04 mM
Incubation Time: 21 h
Result: Significantly increased and maintained Nanog expression in culture. (Nanog: a divergent homeoprotein pluripotency sustaining factor for ESCs that drives ESC self-renewal).
Exhibited the maintenance of murine ESC pluripotency were independent of the LIF/Stat3 pathway.

Western Blot Analysis[1]

Cell Line: P19 cells
Concentration: 10 µM
Incubation Time: 24 h
Result: Significantly reduced coimmunoprecipitation of PR72/130 with both PP2A and Nkd.
Binded to PR72/130 subunit of PP2A, and disrupted the PP2A/Nkd complex.
Decreased the affinity of the β-catenin/p300 interaction and increases β-catenin/CBP usage by negatively regulating the phosphorylation of p300 Ser-89.
Molecular Weight

362.42

Formula

C21H22N4O2

CAS No.
SMILES

CC(C1=CC=C(/N=N/C(C(N)=O)=C2NC(C)(C)CC3=CC=CC=C3/2)C=C1)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 33 mg/mL (91.05 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7592 mL 13.7961 mL 27.5923 mL
5 mM 0.5518 mL 2.7592 mL 5.5185 mL
10 mM 0.2759 mL 1.3796 mL 2.7592 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 1.67 mg/mL (4.61 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
References
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IQ 1
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