1. Metabolic Enzyme/Protease
  2. HIF/HIF Prolyl-Hydroxylase
  3. KC7F2

KC7F2 

Cat. No.: HY-18777 Purity: >99.0%
Handling Instructions

KC7F2 is a potent hypoxia inducible factor-1 (HIF-1) pathway inhibitor with an IC50 of 20 μM in LN229-HRE-AP cells, and with potential as a cancer therapy agent.

For research use only. We do not sell to patients.

KC7F2 Chemical Structure

KC7F2 Chemical Structure

CAS No. : 927822-86-4

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Free Sample (0.5-1 mg)   Apply Now  
10 mM * 1 mL in DMSO USD 63 In-stock
Estimated Time of Arrival: December 31
5 mg USD 50 In-stock
Estimated Time of Arrival: December 31
10 mg USD 60 In-stock
Estimated Time of Arrival: December 31
50 mg USD 210 In-stock
Estimated Time of Arrival: December 31
100 mg USD 310 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 4 publication(s) in Google Scholar

Top Publications Citing Use of Products

    KC7F2 purchased from MCE. Usage Cited in: BMC Anesthesiol. 2019 Jul 9;19(1):127.

    The hCMEC/D3 cells are treated with TNF-α, propofol, and Hif-1α inhibitor (KC7F2, 10 μM). Left side shows the image of a representative Western blot for Hif-1α, VEGF, p-VEGFR-2, p-ERK, ERK and occludin. Right side is the plot of normalized ratios of optical densities. β-actin is served as internal loading control.
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    Description

    KC7F2 is a potent hypoxia inducible factor-1 (HIF-1) pathway inhibitor with an IC50 of 20 μM in LN229-HRE-AP cells, and with potential as a cancer therapy agent[1].

    IC50 & Target

    IC50: 20 μM (HIF-1, LN229-HRE-AP cells)[1]

    In Vitro

    KC7F2 (15–25 μM; 0-72 hours) exhibits a clear dose-response cytotoxicity with an IC50 value of approximately 15–25 μM depending on the cell lines, and this effect is more severe under hypoxic conditions[1].
    KC7F2 (0-80 μM; 6 hours) specifically reduces the protein levels of HIF-1α in a dose-dependent manner under hypoxic conditions; strongly decrease in HIF-1α levels at concentrations above 20 μM[1].
    KC7F2 does not affect the rate of HIF-1α protein degradation[1].
    KC7F2 inhibits HIF-1α protein synthesis but not its mRNA transcription[1].
    KC7F2 represses the phosphorylation of eukaryotic initiation factor 4E binding protein 1 (4EBP1)[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Cell Cytotoxicity Assay[1]

    Cell Line: MCF7 cells, LNZ308 cells, A549 cells, U251MG cells, LN229 cells
    Concentration: 15–25 μM
    Incubation Time: 0-72 hours
    Result: With cytotoxicity more pronounced in tumor cell lines as compared to normal cells.

    Cell Viability Assay[1]

    Cell Line: LN229 cells
    Concentration: 6 hours
    Incubation Time: 0 μM, 5 μM,7.5 μM,10 μM,15 μM,20 μM,30 μM,40 μM,60 μM,80 μM
    Result: Decreases HIF-1α protein levels in a dose-dependent manner.
    Molecular Weight

    570.38

    Formula

    C₁₆H₁₆Cl₄N₂O₄S₄

    CAS No.

    927822-86-4

    SMILES

    O=S(C1=CC(Cl)=CC=C1Cl)(NCCSSCCNS(C2=CC(Cl)=CC=C2Cl)(=O)=O)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 32 mg/mL (56.10 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7532 mL 8.7661 mL 17.5322 mL
    5 mM 0.3506 mL 1.7532 mL 3.5064 mL
    10 mM 0.1753 mL 0.8766 mL 1.7532 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: 2.5 mg/mL (4.38 mM); Clear solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (4.38 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
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    Keywords:

    KC7F2HIF/HIF Prolyl-HydroxylaseHypoxia-inducible factorsHIFsHIF-PHInhibitorinhibitorinhibit

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    Product Name:
    KC7F2
    Cat. No.:
    HY-18777
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