Cat. No.: HY-N2466

Melanotan I

(Synonyms: MT-I; [Nle4,D-Phe7]-α-MSH)

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

Melanotan I (MT-I; [Nle4,D-Phe7]-α-MSH) is an α-MSH (HY-P0252) analog, synthetic melanotropic peptide, and melanocortin receptor (MCR) agonist. Melanotan I induces skin tanning by mimicking the action of α-MSH on the melanocortin 1 receptor (MC1R) in melanocytes. As an appetite suppressant, Melanotan I impairs the ability of insulin to inhibit endogenous glucose production, suppresses leptin secretion from adipose tissue, and thereby reduces voluntary feed intake in ewes. Melanotan I can be used in research related to UV-induced skin damage, polymorphic light eruption, ruminant feeding, and sexual dysfunction.

For research use only. We do not sell to patients.

Purity: 99.04%
CAS No.: 75921-69-6
Formula: C₇₈H₁₁₁N₂₁O₁₉
Molecular Weight:1646.85

Storage:

Sealed storage, away from moisture.

Powder -80°C, 2 years , -20°C, 1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Melanotan I Chemical Structure

CAS No.: 75921-69-6

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (1)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
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Bioz

Biological Activity

Description

IC₅₀ & Target

MC1R

All Melanocortin Receptor Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	1.5 × 10^-10 M Compound: NDP-alpha-MSH	Displacement of [125I]NDP-alpha-MSH from human recombinant MC4 receptor expressed in CHO cells Displacement of [125I]NDP-alpha-MSH from human recombinant MC4 receptor expressed in CHO cells	[PMID: 26988801]
CHO	IC₅₀	1.5 x 10^-10 M Compound: NDP-alpha-MSH	Displacement of [125I]NDP-a-MSH from human recombinant MC4 receptor expressed in CHO cells measured after 120 mins by scintillation counting method Displacement of [125I]NDP-a-MSH from human recombinant MC4 receptor expressed in CHO cells measured after 120 mins by scintillation counting method	[PMID: 27876250]
HEK293	EC₅₀	0.038 nM Compound: NDP-MSH	Activity in mouse melanocortin-1 receptor stably expressed in HEK293 cells Activity in mouse melanocortin-1 receptor stably expressed in HEK293 cells	[PMID: 12477357]
HEK293	EC₅₀	0.071 nM Compound: NDP-MSH	Activity in mouse melanocortin-5 receptor stably expressed in HEK293 cells Activity in mouse melanocortin-5 receptor stably expressed in HEK293 cells	[PMID: 12477357]
HEK293	EC₅₀	0.098 nM Compound: NDP-MSH	Activity in mouse melanocortin-3 receptor (mMC3R) stably expressed in HEK293 cells Activity in mouse melanocortin-3 receptor (mMC3R) stably expressed in HEK293 cells	[PMID: 12477357]
HEK293	EC₅₀	0.21 nM Compound: NDP-MSH	Activity in mouse melanocortin-4 receptor stably expressed in HEK293 cells Activity in mouse melanocortin-4 receptor stably expressed in HEK293 cells	[PMID: 12477357]
HEK293	EC₅₀	0.012 μM Compound: NDP-MSH	Agonist activity on mouse melanocortin 1 receptor (mMC1R) stably expressed in HEK cells Agonist activity on mouse melanocortin 1 receptor (mMC1R) stably expressed in HEK cells	[PMID: 12781199]
HEK293	EC₅₀	0.075 μM Compound: NDP-MSH	Compound was evaluated for its agonist activity on mouse melanocortin receptor 3 (mMC3R) stably expressed in HEK cells Compound was evaluated for its agonist activity on mouse melanocortin receptor 3 (mMC3R) stably expressed in HEK cells	[PMID: 12781199]
HEK293	EC₅₀	0.07 nM Compound: MT-I	In vitro agonist potency was evaluated in HEK293 cells transfected with human melanocortin receptor (hMC1R) In vitro agonist potency was evaluated in HEK293 cells transfected with human melanocortin receptor (hMC1R)	[PMID: 12873485]
HEK293	EC₅₀	0.36 nM Compound: MT-I	In vitro agonist potency was evaluated in HEK293 cells transfected with human melanocortin receptor (hMC3R) In vitro agonist potency was evaluated in HEK293 cells transfected with human melanocortin receptor (hMC3R)	[PMID: 12873485]
HEK293	EC₅₀	0.51 nM Compound: MT-I	In vitro agonist potency was evaluated in HEK293 cells transfected with human melanocortin receptor (hMC4R) In vitro agonist potency was evaluated in HEK293 cells transfected with human melanocortin receptor (hMC4R)	[PMID: 12873485]
HEK293	EC₅₀	0.023 nM Compound: NDP-MSH	Agonist activity at mouse MC1R expressed in HEK293 cells by beta-galactosidase assay Agonist activity at mouse MC1R expressed in HEK293 cells by beta-galactosidase assay	[PMID: 15615519]
HEK293	EC₅₀	0.062 nM Compound: NDP-MSH	Agonist activity at mouse MC5R expressed in HEK293 cells by beta-galactosidase assay Agonist activity at mouse MC5R expressed in HEK293 cells by beta-galactosidase assay	[PMID: 15615519]
HEK293	EC₅₀	0.085 nM Compound: NDP-MSH	Agonist activity at mouse MC3R expressed in HEK293 cells by beta-galactosidase assay Agonist activity at mouse MC3R expressed in HEK293 cells by beta-galactosidase assay	[PMID: 15615519]
HEK293	EC₅₀	0.13 nM Compound: NDP-MSH	Agonist activity at mouse MC4R expressed in HEK293 cells by beta-galactosidase assay Agonist activity at mouse MC4R expressed in HEK293 cells by beta-galactosidase assay	[PMID: 15615519]
HEK293	EC₅₀	0.2 nM Compound: 15	Effective concentration for intracellular cAMP accumulation in human melanocortin 4 receptor expressing HEK 293 cells; (N = 4) Effective concentration for intracellular cAMP accumulation in human melanocortin 4 receptor expressing HEK 293 cells; (N = 4)	[PMID: 15771429]
HEK293	EC₅₀	0.8 nM Compound: 15	Effective concentration for intracellular cAMP accumulation in human melanocortin 3 receptor expressing HEK 293 cells; (N = 4) Effective concentration for intracellular cAMP accumulation in human melanocortin 3 receptor expressing HEK 293 cells; (N = 4)	[PMID: 15771429]
HEK293	EC₅₀	1 nM Compound: 15	Effective concentration for intracellular cAMP accumulation in human melanocortin 5 receptor expressing HEK 293 cells; (N = 4) Effective concentration for intracellular cAMP accumulation in human melanocortin 5 receptor expressing HEK 293 cells; (N = 4)	[PMID: 15771429]
HEK293	IC₅₀	0.4 nM Compound: 15	Inhibition of [125I]NDP-MSH binding to Melanocortin 4 receptor expressed in HEK293 cells; (N = 4) Inhibition of [125I]NDP-MSH binding to Melanocortin 4 receptor expressed in HEK293 cells; (N = 4)	[PMID: 15771429]
HEK293	IC₅₀	2.2 nM Compound: 15	Inhibition of [125I]NDP-MSH binding to Melanocortin 5 receptor expressed in HEK293 cells; N = 4 Inhibition of [125I]NDP-MSH binding to Melanocortin 5 receptor expressed in HEK293 cells; N = 4	[PMID: 15771429]
HEK293	IC₅₀	3.3 nM Compound: 15	Inhibition of [125I]NDP-MSH binding to Melanocortin 3 receptor expressed in HEK293 cells; (N = 4) Inhibition of [125I]NDP-MSH binding to Melanocortin 3 receptor expressed in HEK293 cells; (N = 4)	[PMID: 15771429]
HEK293	EC₅₀	0.51 nM Compound: NDP-alpha-MSH	Agonist activity at the mutant MC4R F184A expressed in HEK293 cells by cAMP accumulation Agonist activity at the mutant MC4R F184A expressed in HEK293 cells by cAMP accumulation	[PMID: 16451057]
HEK293	EC₅₀	1 nM Compound: NDP-alpha-MSH	Agonist activity at the mutant MC4R Y287F expressed in HEK293 cells by cAMP accumulation Agonist activity at the mutant MC4R Y287F expressed in HEK293 cells by cAMP accumulation	[PMID: 16451057]
HEK293	EC₅₀	1.1 nM Compound: NDP-alpha-MSH	Agonist activity at the mutant MC4R F184L expressed in HEK293 cells by cAMP accumulation Agonist activity at the mutant MC4R F184L expressed in HEK293 cells by cAMP accumulation	[PMID: 16451057]
HEK293	EC₅₀	1.1 nM Compound: NDP-alpha-MSH	Agonist activity at the mutant MC4R I129A expressed in HEK293 cells by cAMP accumulation Agonist activity at the mutant MC4R I129A expressed in HEK293 cells by cAMP accumulation	[PMID: 16451057]
HEK293	EC₅₀	1.2 nM Compound: NDP-alpha-MSH	Agonist activity at the human wild type MC4R expressed in HEK293 cells by cAMP accumulation Agonist activity at the human wild type MC4R expressed in HEK293 cells by cAMP accumulation	[PMID: 16451057]
HEK293	EC₅₀	1.4 nM Compound: NDP-alpha-MSH	Agonist activity at the mutant MC4R I125A expressed in HEK293 cells by cAMP accumulation Agonist activity at the mutant MC4R I125A expressed in HEK293 cells by cAMP accumulation	[PMID: 16451057]
HEK293	EC₅₀	1.5 nM Compound: NDP-alpha-MSH	Agonist activity at the mutant MC4R Y268F expressed in HEK293 cells by cAMP accumulation Agonist activity at the mutant MC4R Y268F expressed in HEK293 cells by cAMP accumulation	[PMID: 16451057]
HEK293	EC₅₀	2 nM Compound: NDP-alpha-MSH	Agonist activity at the mutant MC4R Y268A expressed in HEK293 cells by cAMP accumulation Agonist activity at the mutant MC4R Y268A expressed in HEK293 cells by cAMP accumulation	[PMID: 16451057]
HEK293	EC₅₀	5.1 nM Compound: NDP-alpha-MSH	Agonist activity at the mutant MC4R I291A expressed in HEK293 cells by cAMP accumulation Agonist activity at the mutant MC4R I291A expressed in HEK293 cells by cAMP accumulation	[PMID: 16451057]
HEK293	EC₅₀	9 nM Compound: NDP-alpha-MSH	Agonist activity at the mutant MC4R Y287A expressed in HEK293 cells by cAMP accumulation Agonist activity at the mutant MC4R Y287A expressed in HEK293 cells by cAMP accumulation	[PMID: 16451057]
HEK293	EC₅₀	0.008 nM Compound: NDP-MSH	Agonist activity at mouse MC1R expressed in HEK293 cells Agonist activity at mouse MC1R expressed in HEK293 cells	[PMID: 18220330]
HEK293	EC₅₀	0.063 nM Compound: NDP-MSH	Agonist activity at mouse MC3R expressed in HEK293 cells Agonist activity at mouse MC3R expressed in HEK293 cells	[PMID: 18220330]
HEK293	EC₅₀	0.078 nM Compound: NDP-MSH	Agonist activity at mouse MC5R expressed in HEK293 cells Agonist activity at mouse MC5R expressed in HEK293 cells	[PMID: 18220330]
HEK293	EC₅₀	0.097 nM Compound: NDP-MSH	Agonist activity at mouse MC4R expressed in HEK293 cells Agonist activity at mouse MC4R expressed in HEK293 cells	[PMID: 18220330]
HEK293	EC₅₀	0.088 nM Compound: NDP-MSH	Agonist activity at mouse MC1R expressed in HEK293 cells by CRE/beta-galactosidase reporter gene assay Agonist activity at mouse MC1R expressed in HEK293 cells by CRE/beta-galactosidase reporter gene assay	[PMID: 18800761]
HEK293	EC₅₀	0.12 nM Compound: NDP-MSH	Agonist activity at mouse MC4R expressed in HEK293 cells by CRE/beta-galactosidase reporter gene assay Agonist activity at mouse MC4R expressed in HEK293 cells by CRE/beta-galactosidase reporter gene assay	[PMID: 18800761]
HEK293	EC₅₀	0.26 nM Compound: NDP-MSH	Agonist activity at mouse MC5R expressed in HEK293 cells by CRE/beta-galactosidase reporter gene assay Agonist activity at mouse MC5R expressed in HEK293 cells by CRE/beta-galactosidase reporter gene assay	[PMID: 18800761]
HEK293	EC₅₀	0.33 nM Compound: NDP-MSH	Agonist activity at mouse MC3R expressed in HEK293 cells by CRE/beta-galactosidase reporter gene assay Agonist activity at mouse MC3R expressed in HEK293 cells by CRE/beta-galactosidase reporter gene assay	[PMID: 18800761]
HEK293	EC₅₀	0.018 nM Compound: NDP-MSH	Agonist activity at mouse melanocortin 1 receptor expressed in HEK293 cells after 48 hrs by cAMP based beta-galactosidase reporter gene assay Agonist activity at mouse melanocortin 1 receptor expressed in HEK293 cells after 48 hrs by cAMP based beta-galactosidase reporter gene assay	[PMID: 21306168]
HEK293	EC₅₀	0.03 nM Compound: NDP-MSH	Agonist activity at mouse melanocortin 5 receptor expressed in HEK293 cells after 48 hrs by cAMP based beta-galactosidase reporter gene assay Agonist activity at mouse melanocortin 5 receptor expressed in HEK293 cells after 48 hrs by cAMP based beta-galactosidase reporter gene assay	[PMID: 21306168]
HEK293	EC₅₀	0.14 nM Compound: NDP-MSH	Agonist activity at mouse melanocortin 3 receptor expressed in HEK293 cells after 48 hrs by cAMP based beta-galactosidase reporter gene assay Agonist activity at mouse melanocortin 3 receptor expressed in HEK293 cells after 48 hrs by cAMP based beta-galactosidase reporter gene assay	[PMID: 21306168]
HEK293	EC₅₀	0.2 nM Compound: NDP-MSH	Agonist activity at mouse melanocortin 4 receptor expressed in HEK293 cells after 48 hrs by cAMP based beta-galactosidase reporter gene assay Agonist activity at mouse melanocortin 4 receptor expressed in HEK293 cells after 48 hrs by cAMP based beta-galactosidase reporter gene assay	[PMID: 21306168]
HEK293	EC₅₀	0.05 nM Compound: NDP-MSH	Agonist activity at mouse MC1 receptor expressed in HEK293 cells after 6 hrs by cAMP-based beta-galactosidase reporter gene assay Agonist activity at mouse MC1 receptor expressed in HEK293 cells after 6 hrs by cAMP-based beta-galactosidase reporter gene assay	[PMID: 23432160]
HEK293	EC₅₀	0.2 nM Compound: NDP-MSH	Agonist activity at mouse MC4 receptor expressed in HEK293 cells after 6 hrs by cAMP-based beta-galactosidase reporter gene assay Agonist activity at mouse MC4 receptor expressed in HEK293 cells after 6 hrs by cAMP-based beta-galactosidase reporter gene assay	[PMID: 23432160]
HEK293	EC₅₀	0.23 nM Compound: NDP-MSH	Agonist activity at mouse MC3 receptor expressed in HEK293 cells after 6 hrs by cAMP-based beta-galactosidase reporter gene assay Agonist activity at mouse MC3 receptor expressed in HEK293 cells after 6 hrs by cAMP-based beta-galactosidase reporter gene assay	[PMID: 23432160]
HEK293	EC₅₀	0.28 nM Compound: NDP-MSH	Agonist activity at mouse MC5 receptor expressed in HEK293 cells after 6 hrs by cAMP-based beta-galactosidase reporter gene assay Agonist activity at mouse MC5 receptor expressed in HEK293 cells after 6 hrs by cAMP-based beta-galactosidase reporter gene assay	[PMID: 23432160]
HEK293	EC₅₀	0.088 nM Compound: NDP-MSH	Agonist activity at mouse melanocortin-1 receptor expressed in HEK293 cells co-transfected with CRE/beta-galactosidase reporter gene assessed as stimulatory response after 6 hrs Agonist activity at mouse melanocortin-1 receptor expressed in HEK293 cells co-transfected with CRE/beta-galactosidase reporter gene assessed as stimulatory response after 6 hrs	[PMID: 24517312]
HEK293	EC₅₀	0.12 nM Compound: NDP-MSH	Agonist activity at mouse melanocortin-4 receptor expressed in HEK293 cells co-transfected with CRE/beta-galactosidase reporter gene assessed as stimulatory response after 6 hrs Agonist activity at mouse melanocortin-4 receptor expressed in HEK293 cells co-transfected with CRE/beta-galactosidase reporter gene assessed as stimulatory response after 6 hrs	[PMID: 24517312]
HEK293	EC₅₀	0.26 nM Compound: NDP-MSH	Agonist activity at mouse melanocortin-5 receptor expressed in HEK293 cells co-transfected with CRE/beta-galactosidase reporter gene assessed as stimulatory response after 6 hrs Agonist activity at mouse melanocortin-5 receptor expressed in HEK293 cells co-transfected with CRE/beta-galactosidase reporter gene assessed as stimulatory response after 6 hrs	[PMID: 24517312]
HEK293	EC₅₀	0.33 nM Compound: NDP-MSH	Agonist activity at mouse melanocortin-3 receptor expressed in HEK293 cells co-transfected with CRE/beta-galactosidase reporter gene assessed as stimulatory response after 6 hrs Agonist activity at mouse melanocortin-3 receptor expressed in HEK293 cells co-transfected with CRE/beta-galactosidase reporter gene assessed as stimulatory response after 6 hrs	[PMID: 24517312]
HEK293	IC₅₀	14 nM Compound: NDP-MSH	Displacement of [I125]-NDP-MSH from wild type human melanocortin-4 receptor expressed in HEK293 cells Displacement of [I125]-NDP-MSH from wild type human melanocortin-4 receptor expressed in HEK293 cells	[PMID: 24517312]
HEK293	IC₅₀	9 nM Compound: NDP-MSH	Displacement of [I125]-NDP-MSH from human melanocortin-4 receptor L106P mutant expressed in HEK293 cells Displacement of [I125]-NDP-MSH from human melanocortin-4 receptor L106P mutant expressed in HEK293 cells	[PMID: 24517312]
HEK293	EC₅₀	0.092 nM Compound: NDP-MSH	Activity at mouse MC1R expressed in HEK293 cells assessed as cAMP accumulation by CRE/beta-galactosidase reporter gene assay Activity at mouse MC1R expressed in HEK293 cells assessed as cAMP accumulation by CRE/beta-galactosidase reporter gene assay	[PMID: 25347033]
HEK293	EC₅₀	0.2 nM Compound: NDP-MSH	Activity at mouse MC4R expressed in HEK293 cells assessed as cAMP accumulation by CRE/beta-galactosidase reporter gene assay Activity at mouse MC4R expressed in HEK293 cells assessed as cAMP accumulation by CRE/beta-galactosidase reporter gene assay	[PMID: 25347033]
HEK293	EC₅₀	0.81 nM Compound: NDP-MSH	Activity at mouse MC3R expressed in HEK293 cells assessed as cAMP accumulation by CRE/beta-galactosidase reporter gene assay Activity at mouse MC3R expressed in HEK293 cells assessed as cAMP accumulation by CRE/beta-galactosidase reporter gene assay	[PMID: 25347033]
HEK293	EC₅₀	0.03 nM Compound: NDP-MSH	Agonist activity at mouse MC1R expressed in HEK293 cells assessed as cAMP accumulation incubated for 2 hrs by alphascreen assay Agonist activity at mouse MC1R expressed in HEK293 cells assessed as cAMP accumulation incubated for 2 hrs by alphascreen assay	[PMID: 26959173]
HEK293	EC₅₀	0.24 nM Compound: NDP-MSH	Agonist activity at mouse MC3R expressed in HEK293 cells assessed as cAMP accumulation incubated for 2 hrs by alphascreen assay Agonist activity at mouse MC3R expressed in HEK293 cells assessed as cAMP accumulation incubated for 2 hrs by alphascreen assay	[PMID: 26959173]
HEK293	EC₅₀	0.31 nM Compound: NDP-MSH	Agonist activity at mouse MC5R expressed in HEK293 cells assessed as cAMP accumulation incubated for 2 hrs by alphascreen assay Agonist activity at mouse MC5R expressed in HEK293 cells assessed as cAMP accumulation incubated for 2 hrs by alphascreen assay	[PMID: 26959173]
HEK293	EC₅₀	0.46 nM Compound: NDP-MSH	Agonist activity at mouse MC4R expressed in HEK293 cells assessed as cAMP accumulation incubated for 2 hrs by alphascreen assay Agonist activity at mouse MC4R expressed in HEK293 cells assessed as cAMP accumulation incubated for 2 hrs by alphascreen assay	[PMID: 26959173]
HEK293	IC₅₀	0.31 nM Compound: NDP-MSH	Displacement of 125I-NDP-MSH from mouse MC1R expressed in HEK293 cells incubated for 1 hr by gamma counting analysis Displacement of 125I-NDP-MSH from mouse MC1R expressed in HEK293 cells incubated for 1 hr by gamma counting analysis	[PMID: 26959173]
HEK293	IC₅₀	1.09 nM Compound: NDP-MSH	Displacement of 125I-NDP-MSH from mouse MC4R expressed in HEK293 cells incubated for 1 hr by gamma counting analysis Displacement of 125I-NDP-MSH from mouse MC4R expressed in HEK293 cells incubated for 1 hr by gamma counting analysis	[PMID: 26959173]
HEK293	IC₅₀	4.18 nM Compound: NDP-MSH	Displacement of 125I-NDP-MSH from mouse MC3R expressed in HEK293 cells incubated for 1 hr by gamma counting analysis Displacement of 125I-NDP-MSH from mouse MC3R expressed in HEK293 cells incubated for 1 hr by gamma counting analysis	[PMID: 26959173]
HEK293	EC₅₀	0.4 nM Compound: NDP-MSH	Agonist activity at EYFP-fused human MC4R expressed in HEK293 cells after 16 to 20 hrs by CRE-driven reporter assay Agonist activity at EYFP-fused human MC4R expressed in HEK293 cells after 16 to 20 hrs by CRE-driven reporter assay	[PMID: 28972753]
HEK293	EC₅₀	1.1 nM Compound: NDP-MSH	Agonist activity at EYFP-fused human MC5R expressed in HEK293 cells after 16 to 20 hrs by CRE-driven reporter assay Agonist activity at EYFP-fused human MC5R expressed in HEK293 cells after 16 to 20 hrs by CRE-driven reporter assay	[PMID: 28972753]
HEK293	EC₅₀	6.4 nM Compound: NDP-MSH	Agonist activity at EYFP-fused human MC4R expressed in HEK293 cells assessed as increase in cAMP accumulation after 30 mins by luminescent enzymatic complementation based assay Agonist activity at EYFP-fused human MC4R expressed in HEK293 cells assessed as increase in cAMP accumulation after 30 mins by luminescent enzymatic complementation based assay	[PMID: 28972753]
HEK293	EC₅₀	6.8 nM Compound: NDP-MSH	Agonist activity at EYFP-fused human MC3R expressed in HEK293 cells after 16 to 20 hrs by CRE-driven reporter assay Agonist activity at EYFP-fused human MC3R expressed in HEK293 cells after 16 to 20 hrs by CRE-driven reporter assay	[PMID: 28972753]
HEK293	EC₅₀	0.07 nM Compound: NDP-MSH	Agonist activity at human MC1R expressed in HEK293 cells assessed as increase in cAMP production incubated for 2 hrs by AlphaScreen assay Agonist activity at human MC1R expressed in HEK293 cells assessed as increase in cAMP production incubated for 2 hrs by AlphaScreen assay	[PMID: 29578343]
HEK293	EC₅₀	0.13 nM Compound: NDP-MSH	Agonist activity at human MC4R expressed in HEK293 cells assessed as increase in cAMP production incubated for 2 hrs by AlphaScreen assay Agonist activity at human MC4R expressed in HEK293 cells assessed as increase in cAMP production incubated for 2 hrs by AlphaScreen assay	[PMID: 29578343]
HEK293	EC₅₀	0.3 nM Compound: NDP-MSH	Agonist activity at human MC3R expressed in HEK293 cells assessed as increase in cAMP production incubated for 2 hrs by AlphaScreen assay Agonist activity at human MC3R expressed in HEK293 cells assessed as increase in cAMP production incubated for 2 hrs by AlphaScreen assay	[PMID: 29578343]
HEK293	EC₅₀	0.4 nM Compound: NDP-MSH	Agonist activity at human MC5R expressed in HEK293 cells assessed as increase in cAMP production incubated for 2 hrs by AlphaScreen assay Agonist activity at human MC5R expressed in HEK293 cells assessed as increase in cAMP production incubated for 2 hrs by AlphaScreen assay	[PMID: 29578343]
HEK293	EC₅₀	0.051 nM Compound: NDP-MSH	Full agonist activity at mouse MC1R expressed in HEK293 cells co-expressing CRE/beta-galactosidase reporter gene after 6 hrs by beta-galactosidase cAMP assay Full agonist activity at mouse MC1R expressed in HEK293 cells co-expressing CRE/beta-galactosidase reporter gene after 6 hrs by beta-galactosidase cAMP assay	[PMID: 30741545]
HEK293	EC₅₀	0.279 nM Compound: NDP-MSH	Agonist activity at mouse MC4R expressed in HEK293 cells co-expressing CRE/beta-galactosidase reporter gene after 6 hrs by beta-galactosidase cAMP assay Agonist activity at mouse MC4R expressed in HEK293 cells co-expressing CRE/beta-galactosidase reporter gene after 6 hrs by beta-galactosidase cAMP assay	[PMID: 30741545]
HEK293	EC₅₀	0.414 nM Compound: NDP-MSH	Agonist activity at mouse MC3R expressed in HEK293 cells co-expressing CRE/beta-galactosidase reporter gene after 6 hrs by beta-galactosidase cAMP assay Agonist activity at mouse MC3R expressed in HEK293 cells co-expressing CRE/beta-galactosidase reporter gene after 6 hrs by beta-galactosidase cAMP assay	[PMID: 30741545]
HEK293	EC₅₀	1.98 nM Compound: NDP-MSH	Full agonist activity at mouse MC5R expressed in HEK293 cells co-expressing CRE/beta-galactosidase reporter gene after 6 hrs by beta-galactosidase cAMP assay Full agonist activity at mouse MC5R expressed in HEK293 cells co-expressing CRE/beta-galactosidase reporter gene after 6 hrs by beta-galactosidase cAMP assay	[PMID: 30741545]
HEK293	EC₅₀	0.01 nM Compound: NDP-MSH	Agonist activity at mouse melanocortin receptor 1 expressed in HEK293 cells assessed as stimulation of cAMP accumulation incubated for 2 hrs by cAMP Alpha-screen assay Agonist activity at mouse melanocortin receptor 1 expressed in HEK293 cells assessed as stimulation of cAMP accumulation incubated for 2 hrs by cAMP Alpha-screen assay	[PMID: 31845801]
HEK293	EC₅₀	0.07 nM Compound: NDP-MSH	Agonist activity at mouse melanocortin receptor 3 expressed in HEK293 cells assessed as stimulation of cAMP accumulation incubated for 2 hrs by cAMP Alpha-screen assay Agonist activity at mouse melanocortin receptor 3 expressed in HEK293 cells assessed as stimulation of cAMP accumulation incubated for 2 hrs by cAMP Alpha-screen assay	[PMID: 31845801]
HEK293	EC₅₀	0.13 nM Compound: NDP-MSH	Agonist activity at mouse melanocortin receptor 5 expressed in HEK293 cells assessed as stimulation of cAMP accumulation incubated for 2 hrs by cAMP Alpha-screen assay Agonist activity at mouse melanocortin receptor 5 expressed in HEK293 cells assessed as stimulation of cAMP accumulation incubated for 2 hrs by cAMP Alpha-screen assay	[PMID: 31845801]
HEK293	EC₅₀	0.9 nM Compound: NDP-MSH	Agonist activity at mouse melanocortin receptor 4 expressed in HEK293 cells assessed as stimulation of cAMP accumulation incubated for 2 hrs by cAMP Alpha-screen assay Agonist activity at mouse melanocortin receptor 4 expressed in HEK293 cells assessed as stimulation of cAMP accumulation incubated for 2 hrs by cAMP Alpha-screen assay	[PMID: 31845801]
HEK293	IC₅₀	0.29 nM Compound: NDP-MSH	Displacement of [125I]-NDP-MSH from mouse melanocortin receptor 1 expressed in HEK293 cell mebranes incubated for 1 hr by automatic gamma counting method Displacement of [125I]-NDP-MSH from mouse melanocortin receptor 1 expressed in HEK293 cell mebranes incubated for 1 hr by automatic gamma counting method	[PMID: 31845801]
HEK293	IC₅₀	1.3 nM Compound: NDP-MSH	Displacement of [125I]-NDP-MSH from mouse melanocortin receptor 4 expressed in HEK293 cell mebranes incubated for 1 hr by automatic gamma counting method Displacement of [125I]-NDP-MSH from mouse melanocortin receptor 4 expressed in HEK293 cell mebranes incubated for 1 hr by automatic gamma counting method	[PMID: 31845801]
HEK293	IC₅₀	10 nM Compound: NDP-MSH	Displacement of [125I]-NDP-MSH from human melanocortin receptor 4 expressed in HEK293 cell mebranes incubated for 1 hr by automatic gamma counting method Displacement of [125I]-NDP-MSH from human melanocortin receptor 4 expressed in HEK293 cell mebranes incubated for 1 hr by automatic gamma counting method	[PMID: 31845801]
HEK293	IC₅₀	140 nM Compound: NDP-MSH	Displacement of [125I]-NDP-MSH from mouse melanocortin receptor 5 expressed in HEK293 cell mebranes incubated for 1 hr by automatic gamma counting method Displacement of [125I]-NDP-MSH from mouse melanocortin receptor 5 expressed in HEK293 cell mebranes incubated for 1 hr by automatic gamma counting method	[PMID: 31845801]
HEK293	IC₅₀	8.2 nM Compound: NDP-MSH	Displacement of [125I]-NDP-MSH from mouse melanocortin receptor 3 expressed in HEK293 cell mebranes incubated for 1 hr by automatic gamma counting method Displacement of [125I]-NDP-MSH from mouse melanocortin receptor 3 expressed in HEK293 cell mebranes incubated for 1 hr by automatic gamma counting method	[PMID: 31845801]
HEK293	EC₅₀	0.012 nM Compound: NDP-MSH	Agonist activity at mouse melanocortin receptor 1 expressed in HEK293 cells assessed as stimulation of cAMP accumulation incubated for 2 hrs by cAMP AlphaScreen assay Agonist activity at mouse melanocortin receptor 1 expressed in HEK293 cells assessed as stimulation of cAMP accumulation incubated for 2 hrs by cAMP AlphaScreen assay	[PMID: 32184956]
HEK293	EC₅₀	0.19 nM Compound: NDP-MSH	Agonist activity at mouse melanocortin receptor 3 expressed in HEK293 cells assessed as stimulation of cAMP accumulation incubated for 2 hrs by cAMP Alpha-screen assay Agonist activity at mouse melanocortin receptor 3 expressed in HEK293 cells assessed as stimulation of cAMP accumulation incubated for 2 hrs by cAMP Alpha-screen assay	[PMID: 32184956]
HEK293	EC₅₀	0.3 nM Compound: NDP-MSH	Agonist activity at mouse melanocortin receptor 5 expressed in HEK293 cells assessed as stimulation of cAMP accumulation incubated for 2 hrs by cAMP Alpha-screen assay Agonist activity at mouse melanocortin receptor 5 expressed in HEK293 cells assessed as stimulation of cAMP accumulation incubated for 2 hrs by cAMP Alpha-screen assay	[PMID: 32184956]
HEK293	EC₅₀	1.1 nM Compound: NDP-MSH	Agonist activity at mouse melanocortin receptor 4 expressed in HEK293 cells assessed as stimulation of cAMP accumulation incubated for 2 hrs by cAMP Alpha-screen assay Agonist activity at mouse melanocortin receptor 4 expressed in HEK293 cells assessed as stimulation of cAMP accumulation incubated for 2 hrs by cAMP Alpha-screen assay	[PMID: 32184956]
HEL	EC₅₀	0.12 μM Compound: NDP-MSH	Agonist activity on mouse melanocortin 4 receptor (mMC4R) stably expressed in HEL cells Agonist activity on mouse melanocortin 4 receptor (mMC4R) stably expressed in HEL cells	[PMID: 12781199]
HEL	EC₅₀	0.17 μM Compound: NDP-MSH	Agonist activity on mouse melanocortin 5 receptor (mMC5R) stably expressed in HEL cells Agonist activity on mouse melanocortin 5 receptor (mMC5R) stably expressed in HEL cells	[PMID: 12781199]
MCR	EC₅₀	0.000015 μM Compound: NDP-MSH	Effective concentration was measured against frog skin MCR cells at 10 e-4 10 e-12 M Concentration. Effective concentration was measured against frog skin MCR cells at 10 e-4 10 e-12 M Concentration.	[PMID: 9216831]

In Vitro

Melanotan I (1.0 μM) does not enhance anchorage-independent clonogenic cell growth, a hallmark of malignancy, in freshly isolated human melanoma cell populations, with unaltered or slightly inhibited colony formation at 1.0 μM^[2].
Melanotan I inhibits proliferation of cultured human melanoma cell lines, demonstrating differentiating (melanogenic) activity^[2].
Melanotan I does not alter melanoma invasiveness in the human amniotic basement membrane model in vitro^[2].
Melanotan I stimulates cAMP production and subsequent eumelanin synthesis in cultured human melanocytes with some polymorphic MC1R variants^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Melanotan I (0.03 nmol/h; intracerebroventricular administration; continuous infusion) reduces voluntary feed intake by 50% in adult Suffolk ewes, increases plasma thyroid hormone levels in a feed intake-independent manner, impairs the inhibitory effect of insulin on endogenous glucose production in undernourished ewes, and decreases plasma leptin levels in moderately undernourished ewes^[1].
Melanotan I (10 μM; topical administration) does not induce skin papillomas in vjun transgenic mice, but causes local skin pigmentation^[2].
Melanotan I (4 mg; subcutaneous injection; single sustained-release injection) increases the eumelanin content in guinea pig skin by 2.5-fold, induces persistent pigmentation lasting up to 10 months, and shows no toxicity^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Suffolk ewes (3.5 yr old, body weight 84-96 kg, body condition score 3.3-4.0)^[1]
Dosage:

0.0003 nmol/h; 0.003 nmol/h; 0.03 nmol/h
Administration:

i.c.v.; continuous; 24-82 h
Result:

Reduced voluntary dry matter intake by 50% (from 2,108 g DM/d to ~1,054 g DM/d) and energy intake from 1.8-fold maintenance to 1.1-fold maintenance; the appetite-suppressant effect was first detected 6-24 h after infusion initiation and sustained for 72 h.
Reduced dry matter intake by ~40% (from 2,170 g to 1,293 g) and energy intake from 1.8-fold maintenance to 1.01-fold maintenance; increased plasma triiodothyronine (T3) from 1.27 ng/mL to 1.60 ng/mL and plasma thyroxine (T4) from 5.50 ng/mL to 8.31 ng/mL in an intake-independent manner, with no effect on plasma glucose, insulin, cortisol, or free fatty acids.
Increased plasma T3, tended to reduce plasma leptin, had no effect on basal plasma glucose, fatty acids, insulin, or glucose entry rate, but blunted insulin-dependent suppression of endogenous glucose production during hyperinsulinemic-euglycemic clamps at both low and high insulin infusion rates.
Reduced plasma leptin with no effect on plasma adiponectin.

Chemical Information

CAS No. 75921-69-6
Appearance Solid
Molecular Weight 1646.85
Formula C₇₈H₁₁₁N₂₁O₁₉
Color White to off-white
Synonyms

MT-I; [Nle4,D-Phe7]-α-MSH
Sequence

Ac-Ser-Tyr-Ser-{Nle}-Glu-His-{d-Phe}-Arg-Trp-Gly-Lys-Pro-Val-NH2
Sequence Shortening

Ac-SYS-{Nle}-EH-{d-Phe}-RWGKPV-NH2
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (1)

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Journal Impact Factor

Theranostics

Salidroside can target both P4HB-mediated inflammation and melanogenesis of the skin. [Abstract]

2020 Aug 13;10(24):11110-11126. PMID: 33042273

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (60.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 50 mg/mL (30.36 mM)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

0.6072 mL

3.0361 mL

6.0722 mL

5 mM

0.1214 mL

0.6072 mL

1.2144 mL

10 mM

0.0607 mL

0.3036 mL

0.6072 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (1.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (1.52 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 100 mg/mL (60.72 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Ehrhardt RA, et al. Effects of the central melanocortin system on feed intake, metabolic hormones and insulin action in the sheep. Journal of animal science. 2023 Jan 03;101:skad398. [Content Brief]

[2]. Hadley ME, et al. Melanocortin peptide therapeutics: historical milestones, clinical studies and commercialization. Peptides. 2006 Apr;27(4):921-30. [Content Brief]

[3]. Ückert S, et al. Melanocortin receptor agonists in the treatment of male and female sexual dysfunctions: results from basic research and clinical studies. Expert opinion on investigational drugs. 2014 Nov;23(11):1477-83. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	0.6072 mL	3.0361 mL	6.0722 mL	15.1805 mL
	5 mM	0.1214 mL	0.6072 mL	1.2144 mL	3.0361 mL
	10 mM	0.0607 mL	0.3036 mL	0.6072 mL	1.5180 mL
	15 mM	0.0405 mL	0.2024 mL	0.4048 mL	1.0120 mL
	20 mM	0.0304 mL	0.1518 mL	0.3036 mL	0.7590 mL
	25 mM	0.0243 mL	0.1214 mL	0.2429 mL	0.6072 mL
	30 mM	0.0202 mL	0.1012 mL	0.2024 mL	0.5060 mL
DMSO	40 mM	0.0152 mL	0.0759 mL	0.1518 mL	0.3795 mL
	50 mM	0.0121 mL	0.0607 mL	0.1214 mL	0.3036 mL
	60 mM	0.0101 mL	0.0506 mL	0.1012 mL	0.2530 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Melanotan I

Peptide Library

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

Publications Citing Use of MedChemExpress (MCE) Melanotan I

All Melanocortin Receptor Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Melanotan I

Peptide Library

Drug Repurposing Compound Library Plus

FDA-Approved Drug Library Plus

Publications Citing Use of MedChemExpress (MCE) Melanotan I

All Melanocortin Receptor Isoforms

Biological Activity

Chemical Information

Publications (1)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Melanotan I Related Classifications

HY-N2466 Related Classifications