1. Immunology/Inflammation
  2. Toll-like Receptor (TLR)

Neoseptin 3 

Cat. No.: HY-U00435
Handling Instructions

Neoseptin 3 is a Toll-like receptor 4/myeloid differentiation factor 2 (mTLR4/MD-2) agonist with an EC50 of 18.5 μM.

For research use only. We do not sell to patients.

Neoseptin 3 Chemical Structure

Neoseptin 3 Chemical Structure

CAS No. : 1622863-21-1

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25 mg USD 360 Get quote

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  • Biological Activity

  • Protocol

  • Technical Information

  • References

Description

Neoseptin 3 is a Toll-like receptor 4/myeloid differentiation factor 2 (mTLR4/MD-2) agonist with an EC50 of 18.5 μM.

IC50 & Target

EC50: 18.5 μM (mTLR4/MD-2) [1]

In Vitro

Neoseptin 3 is a Toll-like receptor 4/myeloid differentiation factor 2 (mTLR4/MD-2) agonist with an EC50 of 18.5 μM. Neoseptin-3 induces TNFα production by macrophages in a concentration-dependent manner. Neoseptin-3 induces phosphorylation of IκB kinases α (IKKα), IKKβ, p38, c-Jun N-terminal kinase (JNK), and ERK, and degradation of IκBα, consistent with activation of MAPK and canonical NF-κB signaling. TANK-binding kinase 1 (TBK1) and IRF3 phosphorylation also increase in response to Neoseptin-3[1].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 2.1071 mL 10.5354 mL 21.0708 mL
5 mM 0.4214 mL 2.1071 mL 4.2142 mL
10 mM 0.2107 mL 1.0535 mL 2.1071 mL
Please refer to the solubility information to select the appropriate solvent.
Kinase Assay
[1]

HEK293T cells are transfected with an NF-κB-dependent luciferase reporter plasmid and clones with stable expression are selected by culture in DMEM containing puromycin. Cells are cotransfected with constructs for mouse or human TLR4 plus mouse or human MD-2, and 2 d later are stimulated with 50 μM Neoseptin-3 or 1 μg/mL LPS for 6 h. Cells are lysed, and luciferase activity is measured[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay
[1]

Cells are seeded onto 96-well plates at 1×105 cells per well and stimulated with Neoseptin-3 [dissolved in DMSO; final DMSO concentrations (≤0.2%) are kept constant in all experiments] for 4 h. Mouse TNFα, IL-6, or IFN-β, or human TNFα in the supernatants are measured by ELISA kits according to the manufacturer’s instructions. Unless otherwise indicated, mouse cells are from wild-type C57BL/6J mice[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

474.59

Formula

C₂₉H₃₄N₂O₄

CAS No.

1622863-21-1

SMILES

CC(C)(C)OC([[email protected]](CCC1=CC=CC=C1)NC(C2=CC(CCC3=CC=C(O)C=C3)=C(N)C=C2)=O)=O

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Neoseptin 3
Cat. No.:
HY-U00435
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