1. Immunology/Inflammation
  2. Toll-like Receptor (TLR)
  3. Neoseptin 3

Neoseptin 3 

Cat. No.: HY-U00435 Purity: >98.0%
Handling Instructions

Neoseptin 3 is a Toll-like receptor 4/myeloid differentiation factor 2 (mTLR4/MD-2) agonist with an EC50 of 18.5 μM.

For research use only. We do not sell to patients.

Neoseptin 3 Chemical Structure

Neoseptin 3 Chemical Structure

CAS No. : 1622863-21-1

Size Price Stock Quantity
10 mM * 1  mL in DMSO USD 209 In-stock
Estimated Time of Arrival: December 31
5 mg USD 190 In-stock
Estimated Time of Arrival: December 31
10 mg USD 340 In-stock
Estimated Time of Arrival: December 31
25 mg USD 720 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Neoseptin 3 is a Toll-like receptor 4/myeloid differentiation factor 2 (mTLR4/MD-2) agonist with an EC50 of 18.5 μM.

IC50 & Target

EC50: 18.5 μM (mTLR4/MD-2) [1]

In Vitro

Neoseptin 3 is a Toll-like receptor 4/myeloid differentiation factor 2 (mTLR4/MD-2) agonist with an EC50 of 18.5 μM. Neoseptin-3 induces TNFα production by macrophages in a concentration-dependent manner. Neoseptin-3 induces phosphorylation of IκB kinases α (IKKα), IKKβ, p38, c-Jun N-terminal kinase (JNK), and ERK, and degradation of IκBα, consistent with activation of MAPK and canonical NF-κB signaling. TANK-binding kinase 1 (TBK1) and IRF3 phosphorylation also increase in response to Neoseptin-3[1].

Molecular Weight

474.59

Formula

C₂₉H₃₄N₂O₄

CAS No.

1622863-21-1

SMILES

CC(C)(C)OC([[email protected]](CCC1=CC=CC=C1)NC(C2=CC(CCC3=CC=C(O)C=C3)=C(N)C=C2)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (210.71 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1071 mL 10.5354 mL 21.0708 mL
5 mM 0.4214 mL 2.1071 mL 4.2142 mL
10 mM 0.2107 mL 1.0535 mL 2.1071 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (5.27 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (5.27 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (5.27 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Kinase Assay
[1]

HEK293T cells are transfected with an NF-κB-dependent luciferase reporter plasmid and clones with stable expression are selected by culture in DMEM containing puromycin. Cells are cotransfected with constructs for mouse or human TLR4 plus mouse or human MD-2, and 2 d later are stimulated with 50 μM Neoseptin-3 or 1 μg/mL LPS for 6 h. Cells are lysed, and luciferase activity is measured[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay
[1]

Cells are seeded onto 96-well plates at 1×105 cells per well and stimulated with Neoseptin-3 [dissolved in DMSO; final DMSO concentrations (≤0.2%) are kept constant in all experiments] for 4 h. Mouse TNFα, IL-6, or IFN-β, or human TNFα in the supernatants are measured by ELISA kits according to the manufacturer’s instructions. Unless otherwise indicated, mouse cells are from wild-type C57BL/6J mice[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

Neoseptin 3Neoseptin3Neoseptin-3Toll-like Receptor (TLR)Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product name

 

Salutation

Applicant name *

 

Email address *

Phone number *

 

Organization name *

Country or Region *

 

Requested quantity *

Remarks

Bulk Inquiry

Inquiry Information

Product name:
Neoseptin 3
Cat. No.:
HY-U00435
Quantity:
MCE Japan Authorized Agent: