Cat. No.: HY-W050026

Copy Link Print Information

Phenylacetylglutamine

(Synonyms: NSC 203800; Phenylacetyl-L-glutamine)

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

Phenylacetylglutamine is a colonic microbial metabolite from amino acid fermentation.

For research use only. We do not sell to patients.

Purity: 98.21%
CAS No.: 28047-15-6
Formula: C₁₃H₁₆N₂O₄
Molecular Weight:264.28

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Phenylacetylglutamine Chemical Structure

CAS No.: 28047-15-6

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Phenylacetylglutamine is a colonic microbial metabolite from amino acid fermentation.

IC₅₀ & Target

Microbial Metabolite

Human Endogenous Metabolite

All Endogenous Metabolite Isoforms :

In Vitro

Phenylacetylglutamine is a colonic microbial metabolite from amino acid fermentation. It results from glutamine conjugation of phenylacetic acid, which is almost exclusively derived from the microbial conversion of phenylalanine^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 28047-15-6
Appearance Solid
Molecular Weight 264.28
Formula C₁₃H₁₆N₂O₄
Color White to off-white
SMILES

O=C([C@H](CCC(N)=O)NC(CC1=CC=CC=C1)=O)O
Synonyms

NSC 203800; Phenylacetyl-L-glutamine
Structure Classification
- Amino acids
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Microorganisms
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (3)

All Cell Proliferation/Viability AssayFlow CytometryIn Vivo Efficacy StudyCell Migration/Invasion Assay

Sort by:

Journal Impact Factor

Cell Metab

Interplay between gut microbial communities and metabolites modulates pan-cancer immunotherapy responses. [Abstract]

2025 Apr 1;37(4):806-823.e6. PMID: 39909032

Phenylacetylglutamine purchased from MedChemExpress. Usage Cited in: Cell Metab. 2025 Apr 1;37(4):806-823.e6. [Abstract]

Phenylacetylglutamine (PAGln, 50 mg/kg/day; i.p.) attenuated the inhibitory effect of anti-PD-1 on tumor growth in C57BL/6 SPF mice.
Respir Res

Phenylacetylglutamine produced from injury lung alveolar epithelial cells promotes the function of BMSCs by regulating NONRATT006276.2/Mapt pathway. [Abstract]

2025 May 24;26(1):196. PMID: 40413470

Phenylacetylglutamine purchased from MedChemExpress. Usage Cited in: Respir Res. 2025 May 24;26(1):196. [Abstract]

BMSCs were treated the metabolites (UA [180, 360, 720 µM], NMDA [5, 50, 100 µM], Phenylacetylglutamine [PAG, 0.32, 3.2, 7.2 µM]) for 48 h respectively, and cell viability was detected by MTT assay. The results showed that Phenylacetylglutamin (PAG, 0.32–7.2 µM; 48 h) was non-toxic to BMSCs.

Phenylacetylglutamine purchased from MedChemExpress. Usage Cited in: Respir Res. 2025 May 24;26(1):196. [Abstract]

Phenylacetylglutamin (PAG, 3.6–7.2 µM; 24 h) significantly promoted the migration ability of BMSCs.

Phenylacetylglutamine purchased from MedChemExpress. Usage Cited in: Respir Res. 2025 May 24;26(1):196. [Abstract]

Phenylacetylglutamin (7.2 µM; 24 h) significantly promoted the migration ability of BMSCs.
Biomolecules

PAGln, an Atrial Fibrillation-Linked Gut Microbial Metabolite, Acts as a Promoter of Atrial Myocyte Injury. [Abstract]

2022 Aug 15;12(8):1120. PMID: 36009014

Phenylacetylglutamine purchased from MedChemExpress. Usage Cited in: Biomolecules. 2022 Aug 15;12(8):1120. [Abstract]

Phenylacetylglutamine (PAGln, 100 μM, 24 h) intervention significantly increased ROS generation (1.44 vs. 0.80 fold change; p = 0.011), and apoptosis (56.25 vs. 49.76 %, p = 0.002) in mouse HL-1 cells.

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (378.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 25 mg/mL (94.60 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.7839 mL

18.9193 mL

37.8387 mL

5 mM

0.7568 mL

3.7839 mL

7.5677 mL

10 mM

0.3784 mL

1.8919 mL

3.7839 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (7.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (7.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (7.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 50 mg/mL (189.19 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Poesen R, et al. Microbiota-Derived Phenylacetylglutamine Associates with Overall Mortality and Cardiovascular Disease in Patients with CKD. J Am Soc Nephrol. 2016 Nov;27(11):3479-3487. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O / DMSO	1 mM	3.7839 mL	18.9193 mL	37.8387 mL	94.5966 mL
	5 mM	0.7568 mL	3.7839 mL	7.5677 mL	18.9193 mL
	10 mM	0.3784 mL	1.8919 mL	3.7839 mL	9.4597 mL
	15 mM	0.2523 mL	1.2613 mL	2.5226 mL	6.3064 mL
	20 mM	0.1892 mL	0.9460 mL	1.8919 mL	4.7298 mL
	25 mM	0.1514 mL	0.7568 mL	1.5135 mL	3.7839 mL
	30 mM	0.1261 mL	0.6306 mL	1.2613 mL	3.1532 mL
	40 mM	0.0946 mL	0.4730 mL	0.9460 mL	2.3649 mL
	50 mM	0.0757 mL	0.3784 mL	0.7568 mL	1.8919 mL
	60 mM	0.0631 mL	0.3153 mL	0.6306 mL	1.5766 mL
	80 mM	0.0473 mL	0.2365 mL	0.4730 mL	1.1825 mL
DMSO	100 mM	0.0378 mL	0.1892 mL	0.3784 mL	0.9460 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Phenylacetylglutamine

3 Cited Publications

100 mg Get Quote

Subscribe to the

MedChemExpress

Newsletter

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Phenylacetylglutamine

Metabolism/Protease Compound Library

Natural Product Library

Human Endogenous Metabolite Compound Library

Peptidomimetic Library

Gut Microbial Metabolite Library

Microbial Metabolite Library

Food-Sourced Compound Library

Human Metabolite Library

Animal-Sourced Natural Product Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

RO5 Drug-like Natural Product Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Endogenous Metabolite Library

Mass Spectrometry Human Metabolite Library

Amino Acid Metabolite Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Phenylacetylglutamine

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Phenylacetylglutamine

Metabolism/Protease Compound Library

Natural Product Library

Human Endogenous Metabolite Compound Library

Peptidomimetic Library

Gut Microbial Metabolite Library

Microbial Metabolite Library

Food-Sourced Compound Library

Human Metabolite Library

Animal-Sourced Natural Product Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

RO5 Drug-like Natural Product Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Endogenous Metabolite Library

Mass Spectrometry Human Metabolite Library

Amino Acid Metabolite Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Phenylacetylglutamine

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (3)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Phenylacetylglutamine Related Classifications

HY-W050026 Related Classifications