2. MAPK/ERK Pathway Stem Cell/Wnt
  3. Ribosomal S6 Kinase (RSK) ERK
  4. Pluripotin

Pluripotin  (Synonyms: SC1)

Cat. No.: HY-10579 Purity: 98.86%
COA Handling Instructions

Pluripotin is a dual inhibitor of ERK1 and RasGAP with KDs of 98 nM and 212 nM, respectively. Pluripotin also inhibits RSK1, RSK2, RSK3, and RSK4 with IC50s of 0.5, 2.5, 3.3, and 10.0 µM, respectively.

For research use only. We do not sell to patients.

Pluripotin Chemical Structure

Pluripotin Chemical Structure

CAS No. : 839707-37-8

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 94 In-stock
Solution
10 mM * 1 mL in DMSO USD 94 In-stock
Solid
2 mg USD 55 In-stock
5 mg USD 77 In-stock
10 mg USD 110 In-stock
25 mg USD 198 In-stock
50 mg USD 308 In-stock
100 mg USD 495 In-stock
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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 publication(s) in Google Scholar

Pluripotin Related Antibodies

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ERK ERK1 ERK2 ERK5 ERK8

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Pluripotin is a dual inhibitor of ERK1 and RasGAP with KDs of 98 nM and 212 nM, respectively. Pluripotin also inhibits RSK1, RSK2, RSK3, and RSK4 with IC50s of 0.5, 2.5, 3.3, and 10.0 µM, respectively.

IC50 & Target[1][2]

ERK1

98 nM (Kd)

RasGAP

212 nM (Kd)

RSK1

0.5 μM (IC50)

RSK2

2.5 μM (IC50)

RSK3

3.3 μM (IC50)

RSK4

10 μM (IC50)

In Vitro

Pluripotin (SC-1) inhibits Abl1, p70S6K, PLK2, RSK1, RSK2, RSK3, RSK4 with IC50s of 0.005, 1.4, 2.2, 0.5, 2.5, 3.3, 10.0 µM,respectively. Pluripotin (SC-1) decreases cell growth for 7 colon tumor cell lines. After a five day exposure to 0.1 µM SC-1, the seven colon tumor lines are evaluated for changes in cell number and viability. There is a statistically significant decrease in cell number but >95% viability[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pluripotin Related Antibodies
Molecular Weight

550.54

Appearance

Solid

Formula

C27H25F3N8O2
CAS No.
SMILES

CN1C(NC2=NC=C3C(N(C)C(N(C4=CC(NC(C5=CC=CC(C(F)(F)F)=C5)=O)=CC=C4C)C3)=O)=N2)=CC(C)=N1
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (45.41 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8164 mL 9.0820 mL 18.1640 mL
5 mM 0.3633 mL 1.8164 mL 3.6328 mL
10 mM 0.1816 mL 0.9082 mL 1.8164 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.54 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (4.54 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation
References
Cell Assay
[2]

The seven colon cancer lines (COLO 205, HCC-2998, HCT-15, HCT-116, HT29, KM12, SW-620) are used. In all experiments, each tumor line is cultured in 60 mm2 tissue culture treated dishes at an initial concentration of 62,500/mL (total 4 mL) before addition of 0.1 µM Pluripotin (SC-1) or an equivalent amount of diluent (DMSO) the next day. Five day exposures are conducted. The final concentration utilized for treatment for all tumor lines is determined by evaluating a range of SC-1 concentrations (0.01 to 10 µM) for sphere formation and any cytotoxic effects in the HCT-116 tumor line. Cell viability is routinely evaluated with the trypan blue exclusion test and is always >95% for concentrations at or below 0.1 µM[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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Pluripotin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

Pluripotin839707-37-8SC1SC 1SC-1Ribosomal S6 Kinase (RSK)ERKS6KExtracellular signal regulated kinasesInhibitorinhibitorinhibit

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