1. MAPK/ERK Pathway
    Stem Cell/Wnt
  2. Ribosomal S6 Kinase (RSK)
    ERK
  3. Pluripotin

Pluripotin (Synonyms: SC1)

Cat. No.: HY-10579 Purity: 98.86%
Handling Instructions

Pluripotin is a dual inhibitor of ERK1 and RasGAP with KDs of 98 nM and 212 nM, respectively. Pluripotin also inhibits RSK1, RSK2, RSK3, and RSK4 with IC50s of 0.5, 2.5, 3.3, and 10.0 µM, respectively.

For research use only. We do not sell to patients.

Pluripotin Chemical Structure

Pluripotin Chemical Structure

CAS No. : 839707-37-8

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10 mM * 1 mL in DMSO USD 85 In-stock
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100 mg USD 450 In-stock
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Description

Pluripotin is a dual inhibitor of ERK1 and RasGAP with KDs of 98 nM and 212 nM, respectively. Pluripotin also inhibits RSK1, RSK2, RSK3, and RSK4 with IC50s of 0.5, 2.5, 3.3, and 10.0 µM, respectively.

IC50 & Target[1][2]

ERK1

98 nM (Kd)

RasGAP

212 nM (Kd)

RSK1

0.5 μM (IC50)

RSK2

2.5 μM (IC50)

RSK3

3.3 μM (IC50)

RSK4

10 μM (IC50)

In Vitro

Pluripotin (SC-1) inhibits Abl1, p70S6K, PLK2, RSK1, RSK2, RSK3, RSK4 with IC50s of 0.005, 1.4, 2.2, 0.5, 2.5, 3.3, 10.0 µM,respectively. Pluripotin (SC-1) decreases cell growth for 7 colon tumor cell lines. After a five day exposure to 0.1 µM SC-1, the seven colon tumor lines are evaluated for changes in cell number and viability. There is a statistically significant decrease in cell number but >95% viability[2].

Molecular Weight

550.54

Formula

C₂₇H₂₅F₃N₈O₂

CAS No.

839707-37-8

SMILES

CN1C(NC2=NC=C3C(N(C)C(N(C4=CC(NC(C5=CC=CC(C(F)(F)F)=C5)=O)=CC=C4C)C3)=O)=N2)=CC(C)=N1

Shipping

Room temperature in continental US; may vary elsewhere

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 33 mg/mL (59.94 mM)

H2O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8164 mL 9.0820 mL 18.1640 mL
5 mM 0.3633 mL 1.8164 mL 3.6328 mL
10 mM 0.1816 mL 0.9082 mL 1.8164 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (4.54 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (4.54 mM); Suspended solution; Need ultrasonic

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (4.54 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[2]

The seven colon cancer lines (COLO 205, HCC-2998, HCT-15, HCT-116, HT29, KM12, SW-620) are used. In all experiments, each tumor line is cultured in 60 mm2 tissue culture treated dishes at an initial concentration of 62,500/mL (total 4 mL) before addition of 0.1 µM Pluripotin (SC-1) or an equivalent amount of diluent (DMSO) the next day. Five day exposures are conducted. The final concentration utilized for treatment for all tumor lines is determined by evaluating a range of SC-1 concentrations (0.01 to 10 µM) for sphere formation and any cytotoxic effects in the HCT-116 tumor line. Cell viability is routinely evaluated with the trypan blue exclusion test and is always >95% for concentrations at or below 0.1 µM[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 98.86%

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