1. PI3K/Akt/mTOR
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  3. T-00127_HEV1

T-00127_HEV1 is a phosphatidylinositol 4-kinase III beta (PI4KB) inhibitor with an IC50 of 60 nM.

For research use only. We do not sell to patients.

T-00127_HEV1 Chemical Structure

T-00127_HEV1 Chemical Structure

CAS No. : 900874-91-1

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10 mM * 1 mL in DMSO
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Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products

2 Publications Citing Use of MCE T-00127_HEV1

View All PI4K Isoform Specific Products:

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

T-00127_HEV1 is a phosphatidylinositol 4-kinase III beta (PI4KB) inhibitor with an IC50 of 60 nM.

IC50 & Target[1]

PI4KB

60 nM (IC50)

In Vitro

T-00127_HEV1 shows more potent anti-poliovirus (PV) activity (EC50 of 0.77 μM) than other candidate compounds (EC50 of 1.7 to 4.7 μM). GW5074 and T-00127_HEV1 almost completely inhibit PI4KB kinase activity at 10 μM (3% and 5% of residual activity, respectively), in contrast to AN-12-H5 (108% of activity [no inhibition])[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

411.50

Formula

C22H29N5O3

CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

CC1=NC2=C(C3=CC=C(OC)C(OC)=C3)C(C)=NN2C(NCCN4CCOCC4)=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (121.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4301 mL 12.1507 mL 24.3013 mL
5 mM 0.4860 mL 2.4301 mL 4.8603 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.08 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (6.08 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.97%

References
Kinase Assay
[1]

The inhibitory effect of T-00127_HEV1 at a concentration of 10 μM on in vitro cellular protein kinase activities is assessed by kinase profiling with an ATP concentration near the Km for each kinase. Inhibitory effects of GW5074, AN-12-H5, and T-00127_HEV1 at a concentration of 10 μM on in vitro PI kinase activities are assessed by the SelectScreen kinase profiling service with an ATP concentration of 10 μM. For T-00127_HEV1, the 50% inhibitory concentration (IC50) for in vitro PI4KB activity is also measured with an ATP concentration of 10 μM[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay
[1]

RD cells (1.0×104 cells per well in 100 μL medium) in a 96-well plate are infected with PV1, EV71, or CVB3 at multiplicities of infection (MOI) of 10, 1.0, and 0.1 at 37°C for 1 h in the absence of T-00127_HEV1. The cells are washed three times with 10% FCS-DMEM, followed by the addition of 100 μL of 10% FCS-DMEM containing 10 or 0 μM T-00127_HEV1. Cells are collected at 16 h p.i., and then viral RNA is extracted from the cells using a viral RNA purification kit. The number of copies of the viral genome is quantified using a real-time PCR system[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4301 mL 12.1507 mL 24.3013 mL 60.7533 mL
5 mM 0.4860 mL 2.4301 mL 4.8603 mL 12.1507 mL
10 mM 0.2430 mL 1.2151 mL 2.4301 mL 6.0753 mL
15 mM 0.1620 mL 0.8100 mL 1.6201 mL 4.0502 mL
20 mM 0.1215 mL 0.6075 mL 1.2151 mL 3.0377 mL
25 mM 0.0972 mL 0.4860 mL 0.9721 mL 2.4301 mL
30 mM 0.0810 mL 0.4050 mL 0.8100 mL 2.0251 mL
40 mM 0.0608 mL 0.3038 mL 0.6075 mL 1.5188 mL
50 mM 0.0486 mL 0.2430 mL 0.4860 mL 1.2151 mL
60 mM 0.0405 mL 0.2025 mL 0.4050 mL 1.0126 mL
80 mM 0.0304 mL 0.1519 mL 0.3038 mL 0.7594 mL
100 mM 0.0243 mL 0.1215 mL 0.2430 mL 0.6075 mL
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T-00127_HEV1 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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T-00127_HEV1
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