T-00127_HEV1
Based on 4 publication(s) in Google Scholar
T-00127_HEV1 is a phosphatidylinositol 4-kinase III beta (PI4KB) inhibitor with an IC50 of 60 nM.
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- Purity: 99.85%
- CAS No.: 900874-91-1
- 화학식: C22H29N5O3
- 분자량:411.50
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보관:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) T-00127_HEV1
More
Biological Activity
제품 설명
IC50 & Target
[1]|
PI4KB 60 nM (IC50) |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| H1-HeLa | CC50 |
>65 μM
Compound: T-00127-HEV1
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Cytotoxicity against human H1HeLa cells
Cytotoxicity against human H1HeLa cells
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[PMID: 23726345] |
| H1-HeLa | EC50 |
2.5 μM
Compound: 2a, T-00127-HEV1
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Antirhinovirus activity in HRVM infected in H1-HeLa cells assessed as inhibition of virus-induced cytopathic effect after 3 days by Cell-Titer Glo viability assay
Antirhinovirus activity in HRVM infected in H1-HeLa cells assessed as inhibition of virus-induced cytopathic effect after 3 days by Cell-Titer Glo viability assay
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[PMID: 25897704] |
| HeLa | CC50 |
>50 μM
Compound: 2a, T-00127-HEV1
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Cytotoxicity against human HeLa cells
Cytotoxicity against human HeLa cells
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[PMID: 25897704] |
| HeLa | EC50 |
3.38 μM
Compound: 2a, T-00127-HEV1
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Anticoxsackie activity in CVB3 strain Nancy infected in human HeLa cells assessed as inhibition of virus-induced cytopathic effect after 4 hrs by XTT assay
Anticoxsackie activity in CVB3 strain Nancy infected in human HeLa cells assessed as inhibition of virus-induced cytopathic effect after 4 hrs by XTT assay
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[PMID: 25897704] |
| RD | CC50 |
>125 μM
Compound: 3
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Cytotoxicity against human RD cells after 2 by CellTiter-Glo luminescent assay
Cytotoxicity against human RD cells after 2 by CellTiter-Glo luminescent assay
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[PMID: 28122178] |
In Vitro
T-00127_HEV1 shows more potent anti-poliovirus (PV) activity (EC50 of 0.77 μM) than other candidate compounds (EC50 of 1.7 to 4.7 μM). GW5074 and T-00127_HEV1 almost completely inhibit PI4KB kinase activity at 10 μM (3% and 5% of residual activity, respectively), in contrast to AN-12-H5 (108% of activity [no inhibition])[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 900874-91-1
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Appearance Solid
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분자량 411.50
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화학식 C22H29N5O3
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Color White to light yellow
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SMILES
CC1=NC2=C(C3=CC=C(OC)C(OC)=C3)C(C)=NN2C(NCCN4CCOCC4)=C1
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (4)
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Journal Impact Factor
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Most Recent
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Cell Res
Oncogenic RAS induces a distinctive form of non-canonical autophagy mediated by the P38-ULK1-PI4KB axis. [Abstract]2025 Jun;35(6):399-422. PMID: 40055523 -
Sci Adv
Golgi-localized phosphatidylinositol 4-kinase β mediates Rab11a activation and trafficking to promote ciliogenesis. [Abstract]2025 Dec 5;11(49):eadw6910. PMID: 41348894 -
J Cell Physiol
2024 Apr;239(4):e31195. PMID: 38230579 -
용액&용해도
In Vitro:
DMSO : 50 mg/mL (121.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.08 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Kinase Assay[1]
The inhibitory effect of T-00127_HEV1 at a concentration of 10 μM on in vitro cellular protein kinase activities is assessed by kinase profiling with an ATP concentration near the Km for each kinase. Inhibitory effects of GW5074, AN-12-H5, and T-00127_HEV1 at a concentration of 10 μM on in vitro PI kinase activities are assessed by the SelectScreen kinase profiling service with an ATP concentration of 10 μM. For T-00127_HEV1, the 50% inhibitory concentration (IC50) for in vitro PI4KB activity is also measured with an ATP concentration of 10 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Assay[1]
RD cells (1.0×104 cells per well in 100 μL medium) in a 96-well plate are infected with PV1, EV71, or CVB3 at multiplicities of infection (MOI) of 10, 1.0, and 0.1 at 37°C for 1 h in the absence of T-00127_HEV1. The cells are washed three times with 10% FCS-DMEM, followed by the addition of 100 μL of 10% FCS-DMEM containing 10 or 0 μM T-00127_HEV1. Cells are collected at 16 h p.i., and then viral RNA is extracted from the cells using a viral RNA purification kit. The number of copies of the viral genome is quantified using a real-time PCR system[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
순도&문서
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Data Sheet (277 KB)
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SDS (583 KB)
- English - EN (583 KB)
- Français - FR (583 KB)
- Deutsch - DE (583 KB)
- Norwegian - NO (583 KB)
- Español - ES (583 KB)
- Swedish - SV (583 KB)
- Italian - IT (583 KB)
- Korean - KR (583 KB)
- Portuguese - PT (583 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4301 mL | 12.1507 mL | 24.3013 mL | 60.7533 mL |
| 5 mM | 0.4860 mL | 2.4301 mL | 4.8603 mL | 12.1507 mL | |
| 10 mM | 0.2430 mL | 1.2151 mL | 2.4301 mL | 6.0753 mL | |
| 15 mM | 0.1620 mL | 0.8100 mL | 1.6201 mL | 4.0502 mL | |
| 20 mM | 0.1215 mL | 0.6075 mL | 1.2151 mL | 3.0377 mL | |
| 25 mM | 0.0972 mL | 0.4860 mL | 0.9721 mL | 2.4301 mL | |
| 30 mM | 0.0810 mL | 0.4050 mL | 0.8100 mL | 2.0251 mL | |
| 40 mM | 0.0608 mL | 0.3038 mL | 0.6075 mL | 1.5188 mL | |
| 50 mM | 0.0486 mL | 0.2430 mL | 0.4860 mL | 1.2151 mL | |
| 60 mM | 0.0405 mL | 0.2025 mL | 0.4050 mL | 1.0126 mL | |
| 80 mM | 0.0304 mL | 0.1519 mL | 0.3038 mL | 0.7594 mL | |
| 100 mM | 0.0243 mL | 0.1215 mL | 0.2430 mL | 0.6075 mL |
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