Ser/Thr Protease Substrate

N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide (Ac-IEPD-pNA) is a colorimetric peptide substrate of granzyme B that allows accurate measurement of granzyme B activity.

MeOSuc-Arg-Pro-Tyr-pNA (S-2586) is a biological active peptide. (chymotrypsin substrate)

MeO-Succ-Arg-Pro-Tyr-AMC TFA is a fluorogenic peptide substrate for chymotrypsin-like proteases that detects protease activity. (measured at 460 nm)

D-Leu-Thr-Arg-pNA is a substrate for γ-Tryptase, and can be used to measure the effects of small molecule inhibitors on γ-Tryptase activity.

Suc-AAPA-pNA is a substrate for chymotrypsin A and elastase. Suc-AAPA-pNA can be used for enzyme activity assay.

Boc-Phe-Ser-Arg-AMC is a trypsin peptide substrate containing a Boc protecting group. Boc-Phe-Ser-Arg-AMC can be degraded by trypsin, releasing AMC (HY-D0027) which has fluorescence activity. Boc-Phe-Ser-Arg-AMC can be used to assess the activity of trypsin.

D-Val-Gly-Arg-pNA acetate is a chromogenic substrate of TPA (tissue plasminogen activator). D-Val-Gly-Arg-pNA can be used to detect the amidolytic activity of TPA I and TPA II.

Boc-Glu(OBzl)-Ala-Arg-AMC is a fluorogenic substrate for coagulation factor XIa and trypsin. The cleavage of the amide bond between arginine and the methylcoumarin amide group releases fluorescent 7-Amino-4-methylcoumarin (HY-D0027).

Boc-Gln-Gly-Arg-AMC is a fluorogenic substrate for kallikrein (PKa), coagulation factor XIIa (FXIIa) and coagulation factor XIa (FXIa). Boc-Gln-Gly-Arg-AMC enables fluorescence-based activity assays for PKa, FXIIa and FXIa.

Suc-Phe-Ala-Ala-Phe-pNA (Suc-FAAF-pNA) is a chromogenic polypeptide substrate that can be used for the quantitative activity assay of intracellular subtilisin (ISP) and neutral metalloprotease (NPS) derived from Saccharomonospora canescens. Suc-Phe-Ala-Ala-Phe-pNA is hydrolyzed by the catalytic triad of mature proteases to release the chromophore pNA. Suc-Phe-Ala-Ala-Phe-pNA is applicable to enzymological studies.

Ala-Phe-Pro-pNA TFA is a chromogenic substrate of tripeptidyl peptidase. Ala-Phe-Pro-pNA TFA can be used to test tripeptidyl peptidase activity.

Boc-Phe-Ser-Arg-AMC TFA is a trypsin peptide substrate containing a Boc protecting group. Boc-Phe-Ser-Arg-AMC TFA can be degraded by trypsin, releasing AMC (HY-D0027) which has fluorescence activity. Boc-Phe-Ser-Arg-AMC TFA can be used to assess the activity of trypsin.

D-Val-Gly-Arg-pNA is a chromogenic substrate of TPA (tissue plasminogen activator). D-Val-Gly-Arg-pNA can be used to detect the amidolytic activity of TPA I and TPA II.

Suc-AAPY-pNA is an oligptide compound and protease substrate. Suc-AAPY-pNA undergoes hydrolysis by proteases at the peptide bond between tyrosine and p-nitroaniline, releasing p-nitroaniline with an absorption peak at OD₄₁₀. Suc-AAPY-pNA functions as a substrate in preclinical assays for measuring activity of acidic, neutral, and alkaline proteases.

H-Gly-Glu-pNA is a chromogenic peptide substrate that can be widely used in enzymatic analysis, including trypsin and urokinase-type plasminogen activator (uPA).

(Z-AR)2Rh110 is a Rhodamine derivative. (Z-AR)2Rh110 can be hydrolyzed by trypsin.

Bz-Pro-Phe-Arg-pNA (Bz-PFR-pNA) is a chromogenic peptide substrate for plasma and glandular Kallikrein, cysteine proteinase (Cruzipain) and Trypsin. Bz-Pro-Phe-Arg-pNA can be used in Factor XII assay.

Cbz-Lys-Arg-pNA is a peptide substrate containing pNA as the chromogenic group. Cbz-Lys-Arg-pNA is widely used in enzymatic analysis, including thrombin, plasmin, factor Xa and Kallikrein.

Abz-AFRFSQ-EDDnp is a substrate for human kallikrein 6 (K_cat/K_m = 38667 s^-1 mM^-1).