(+)-Catechin hydrate
Based on 21 publication(s) in Google Scholar
(+)-Catechin hydrate inhibits cyclooxygenase-1 (COX-1) with an IC50 of 1.4 μM.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté : 99.59%
- CAS No.: 225937-10-0
- Formule: C15H14O6.xH2O
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Stockage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) (+)-Catechin hydrate
More- Autophagy. 2021 Apr;17(4):872-887. [Abstract]
- Acta Pharm Sin B. 2021 Jan;11(1):143-155. [Abstract]
- Biomaterials. 2021 Aug:275:120952. [Abstract]
- Cell Death Dis. 2024 Dec 26;15(12):932. [Abstract]
- Phytomedicine. 2025 May:140:156549. [Abstract]
- Phytomedicine. 2024 Jul 25:130:155733. [Abstract]
- Food Res Int. 2025 Oct:218:116882. [Abstract]
- Antioxidants (Basel). 2024 Jul 28;13(8):909. [Abstract]
- ACS Omega. 2024 May 17;9(22):23984-23997. [Abstract]
- Plants. 2021 Nov 20;10(11):2525. [Abstract]
- Plant Cell Physiol. 2020 Feb 1;61(2):318-330. [Abstract]
- J Food Meas Charact. 2026 Jun 26.
- Toxicol Appl Pharmacol. 2024 Sep:490:117036. [Abstract]
- Vet Microbiol. 2026 May:316:110992. [Abstract]
- Cancer Rep (Hoboken). 2025 May;8(5):e70169. [Abstract]
- Biochem Biophys Res Commun. 2023 Dec 20:687:149196. [Abstract]
- Biochem Biophys Res Commun. 2018 Sep 3;503(1):297-303. [Abstract]
- Rapid Commun Mass Spectrom. 2025 Dec 15;39(23):e10134. [Abstract]
- Biomed Chromatogr. 2025 Nov;39(11):e70225. [Abstract]
- SSRN. 2023 Jul 14.
- Int J Insect Sci. 2018 Feb 28:10:1179543318758409. [Abstract]
Customer Validation & Images
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Cell Proliferation/Viability Assay
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In Vivo Efficacy Study
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Histological Imaging/Staining
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IHC
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Cell Proliferation/Viability Assay
Activité biologique
Description
IC50 & Target
[1]|
COX-1 1.4 μM (IC50) |
In Vitro
(+)-Catechin exhibits >95% inhibitory activity at 70 μg/mL against cyclooxygenase-1 (COX-1) with an IC50 of 1.4 μM[1]. A dose-dependent reduction in color is observed after 24 hours of treatment with (+)-Catechin, and 54.76% of the cells are dead at the highest concentration of (+)-Catechin tested (160 μg/mL) whereas the IC50 of (+)-Catechin is achieved at 127.62 μg/mL (+)-Catechin. A dose- and time-dependent increase in the induction of apoptosis is observed when MCF-7 cells are treated with (+)-Catechin. When compare to the control cells at 24 hours, 40.7 and 41.16% of the cells treated with 150 μg/mL and 300 μg/mL (+)-Catechin, respectively, undergo apoptosis. The expression levels of Caspase-3, -8, and -9 and p53 in MCF-7 cells treated with 150 μg/mL (+)-Catechin for 24 h increase by 5.81, 1.42, 3.29, and 2.68 fold, respectively, as compare to the levels in untreated control cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Essai clinique
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 225937-10-0
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Appearance Solid
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Formule C15H14O6.xH2O
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Color Light yellow to orange
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SMILES
OC1=CC(O)=C(C[C@H](O)[C@@H](C2=CC=C(O)C(O)=C2)O3)C3=C1.[xH2O]
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (21)
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Journal Impact Factor
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Most Recent
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Autophagy
Sulforaphane Activates a lysosome-dependent transcriptional program to mitigate oxidative stress. [Abstract]2021 Apr;17(4):872-887. PMID: 32138578 -
Acta Pharm Sin B
Chrysin serves as a novel inhibitor of DGK α/FAK interaction to suppress the malignancy of esophageal squamous cell carcinoma (ESCC). [Abstract]2021 Jan;11(1):143-155. PMID: 33532186 -
Biomaterials
Polyphenol-assisted facile assembly of bioactive nanoparticles for targeted therapy of heart diseases. [Abstract]2021 Aug:275:120952. PMID: 34147720 -
Cell Death Dis
TRIM59/RBPJ positive feedback circuit confers gemcitabine resistance in pancreatic cancer by activating the Notch signaling pathway. [Abstract]2024 Dec 26;15(12):932. PMID: 39725730
(+)-Catechin hydrate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Dec 26;15(12):932. [Abstract]
Cell viability assay of PC cells treated with Y-39983, Catechin (1-100 μM; 24 h), and avatrombopag, respectively.
(+)-Catechin hydrate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Dec 26;15(12):932. [Abstract]
Catechin (40 mg/kg; ip; twice a week ) in Six-week-old female BALB/c nude mice (PC cells) decreased tumors in all mice.
(+)-Catechin hydrate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Dec 26;15(12):932. [Abstract]
The organs (heart, liver, lungs, and kidneys) obtained from the indicated mice were used for H&E staining. Catechin (40 mg/kg; ip; twice a week ) in Six-week-old female BALB/c nude mice (PC cells).
(+)-Catechin hydrate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Dec 26;15(12):932. [Abstract]
The tumor slices were subjected to IHC staining for Ki-67 and cleaved caspase 3 levels detection and TUNEL assay. Catechin (40 mg/kg; ip; twice a week ) in Six-week-old female BALB/c nude mice (PC cells).
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Phytomedicine
2025 May:140:156549. PMID: 40023969 -
Phytomedicine
Targeting macrophagic RasGRP1 with catechin hydrate ameliorates sepsis-induced multiorgan dysfunction. [Abstract]2024 Jul 25:130:155733. PMID: 38759314 -
Food Res Int
Effect of tea polyphenol binding to soybean β-conglycinin and glycinin on structure, aggregation, allergenicity and in vitro digestion characteristics. [Abstract]2025 Oct:218:116882. PMID: 40790684 -
Antioxidants (Basel)
Evaluation of In Vitro-Derived Hop Plantlets, cv. Columbus and Magnum, as Potential Source of Bioactive Compounds. [Abstract]2024 Jul 28;13(8):909. PMID: 39199155 -
ACS Omega
Systematic Studies on the Anti-SARS-CoV-2 Mechanisms of Tea Polyphenol-Related Natural Products. [Abstract]2024 May 17;9(22):23984-23997. PMID: 38854515 -
Plants
Metabolites of Prickly Rose: Chemodiversity and Digestive-Enzyme-Inhibiting Potential of Rosa acicularis and the Main Ellagitannin Rugosin D. [Abstract]2021 Nov 20;10(11):2525. PMID: 34834888 -
Plant Cell Physiol
CsMYB3 and CsRuby1 form an 'Activator-and-Repressor' Loop for the Regulation of Anthocyanin Biosynthesis in Citrus. [Abstract]2020 Feb 1;61(2):318-330. PMID: 31642503 -
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Toxicol Appl Pharmacol
Effects of catechin on the malignant biological behavior of gastric cancer cells through the PI3K/Akt signaling pathway. [Abstract]2024 Sep:490:117036. PMID: 39009138
(+)-Catechin hydrate purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2024 Sep:490:117036. [Abstract]
(A-B) CCK-8 test was employed to determine the rate at which various doses of Catechin (0, 80, 160, 240, 320, and 400 μmol/L; 24 h) inhibited the growth of gastric cancer cells. (C & D) CCK-8 test was utilized to measure the capacity of gastric cancer cells treated with various catechin doses to proliferate.
(+)-Catechin hydrate purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2024 Sep:490:117036. [Abstract]
Catechin (60, 90, 120 μM; 24 h) increased the apoptosis rate of SGC-7901 cells in a dose-dependent manner.
(+)-Catechin hydrate purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2024 Sep:490:117036. [Abstract]
(A-D) Wound-healing assay was used to detect the migration rate of gastric cancer cells at 24, 48, and 72 h after treatment with Catechin (60, 90, 120 μM) in SGC-7901 and MGC-803 cells.
(+)-Catechin hydrate purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2024 Sep:490:117036. [Abstract]
Catechin (60, 90, 120 μM; 24 h) in SGC-7901 cells decreased the expression levels of total AKT, p-AKT, and PI3K. Western blot assay was used to detect the protein expression levels of AKT, p-AKT, PI3K and p-AKT/AKT in the PI3K/Akt pathway.
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Vet Microbiol
The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]2026 May:316:110992. PMID: 41865607 -
Cancer Rep (Hoboken)
Unraveling the Anti-Tumor Effects and Molecular Mechanisms of Hairyvein Agrimonia Herb in Gastric Cancer Through Network Pharmacology and Experimental Validation. [Abstract]2025 May;8(5):e70169. PMID: 40391580 -
Biochem Biophys Res Commun
Chinese herb related molecules Catechins, Caudatin and Cucurbitacin-I inhibit the proliferation of glioblastoma by activating KDELR2-mediated endoplasmic reticulum stress. [Abstract]2023 Dec 20:687:149196. PMID: 37939504 -
Biochem Biophys Res Commun
Effects of intracellular iron overload on cell death and identification of potent cell death inhibitors. [Abstract]2018 Sep 3;503(1):297-303. PMID: 29890135 -
Rapid Commun Mass Spectrom
Chemical Profiling of Chixiaodou Danggui San Using Liquid Chromatography-Tandem Mass Spectrometry With Data Mining Strategy. [Abstract]2025 Dec 15;39(23):e10134. PMID: 40907972 -
Biomed Chromatogr
Investigation Into the Chemical Constituents and Cytotoxic Effects on Lung Cancer Cells of the Rhizomes of Polygonum bistorta L. and Polygonum paleaceum Wall. [Abstract]2025 Nov;39(11):e70225. PMID: 40984030 -
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Int J Insect Sci
Do Bioflavonoids in Juniperus virginiana Heartwood Stimulate Oviposition in the Ladybird Coleomegilla maculata?. [Abstract]2018 Feb 28:10:1179543318758409. PMID: 29531477
Solvant et solubilité
In Vitro:
DMSO : 50 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL; Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL; Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocole
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Pureté et documentation
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Fiche technique (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Waffo-Téguo P, et al. Potential cancer-chemopreventive activities of wine stilbenoids and flavans extracted from grape (Vitis vinifera) cell cultures. Nutr Cancer. 2001;40(2):173-9. [Content Brief]
[2]. Alshatwi AA. Catechin hydrate suppresses MCF-7 proliferation through TP53/Caspase-mediated apoptosis. J Exp Clin Cancer Res. 2010 Dec 17;29:167. [Content Brief]
[3]. Cheruku SP, et al. Catechin ameliorates doxorubicin-induced neuronal cytotoxicity in in vitro and episodic memory deficit in in vivo in Wistar rats. Cytotechnology. 2018 Feb;70(1):245-259. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
(+)-Catechin hydrate Related Classifications
HY-N0355 Related Classifications
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