Catechin hydrate (Synonyms: (+)-Catechin hydrate; Cianidanol hydrate; Catechuic acid hydrate)

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Catechin hydrate inhibits cyclooxygenase-1 (COX-1) with an IC₅₀ of 1.4 μM.

For research use only. We do not sell to patients.

CAS No. : 88191-48-4

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Other In-stock Forms of Catechin hydrate:

(+)-Catechin hydrate Catechin

Other Forms of Catechin hydrate:

(+)-Catechin hydrate In-stock
Catechin In-stock

20 Publications Citing Use of MCE Catechin hydrate

Cell Proliferation/Viability Assay

In Vivo Efficacy Study

Apoptosis Analysis

Histological Imaging/Staining

Cell Migration/Invasion Assay

IHC

: Catechin hydrate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Dec 26;15(12):932. [Abstract]; Cell viability assay of PC cells treated with Y-39983, Catechin (1-100 μM; 24 h), and avatrombopag, respectively.

: Catechin hydrate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Dec 26;15(12):932. [Abstract]; Catechin (40 mg/kg; ip; twice a week ) in Six-week-old female BALB/c nude mice (PC cells) decreased tumors in all mice.

: Catechin hydrate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Dec 26;15(12):932. [Abstract]; The organs (heart, liver, lungs, and kidneys) obtained from the indicated mice were used for H&E staining. Catechin (40 mg/kg; ip; twice a week ) in Six-week-old female BALB/c nude mice (PC cells).

: Catechin hydrate purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Dec 26;15(12):932. [Abstract]; The tumor slices were subjected to IHC staining for Ki-67 and cleaved caspase 3 levels detection and TUNEL assay. Catechin (40 mg/kg; ip; twice a week ) in Six-week-old female BALB/c nude mice (PC cells).

: Catechin hydrate purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2024 Jul 14:490:117036. [Abstract]; (A-B) CCK-8 test was employed to determine the rate at which various doses of Catechin (0, 80, 160, 240, 320, and 400 μmol/L; 24 h) inhibited the growth of gastric cancer cells. (C & D) CCK-8 test was utilized to measure the capacity of gastric cancer cells treated with various catechin doses to proliferate.

: Catechin hydrate purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2024 Jul 14:490:117036. [Abstract]; Catechin (60, 90, 120 μM; 24 h) increased the apoptosis rate of SGC-7901 cells in a dose-dependent manner.

: Catechin hydrate purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2024 Jul 14:490:117036. [Abstract]; (A-D) Wound-healing assay was used to detect the migration rate of gastric cancer cells at 24, 48, and 72 h after treatment with Catechin (60, 90, 120 μM) in SGC-7901 and MGC-803 cells.

: Catechin hydrate purchased from MedChemExpress. Usage Cited in: Toxicol Appl Pharmacol. 2024 Jul 14:490:117036. [Abstract]; Catechin (60, 90, 120 μM; 24 h) in SGC-7901 cells decreased the expression levels of total AKT, p-AKT, and PI3K. Western blot assay was used to detect the protein expression levels of AKT, p-AKT, PI3K and p-AKT/AKT in the PI3K/Akt pathway.

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Catechin hydrate inhibits cyclooxygenase-1 (COX-1) with an IC₅₀ of 1.4 μM.

IC₅₀ & Target^[1]

COX-1

1.4 μM (IC₅₀)

In Vitro

Catechin exhibits >95% inhibitory activity at 70 μg/mL against cyclooxygenase-1 (COX-1) with an IC₅₀ of 1.4 μM^[1]. A dose-dependent reduction in color is observed after 24 hours of treatment with Catechin, and 54.76% of the cells are dead at the highest concentration of Catechin tested (160 μg/mL) whereas the IC₅₀ of Catechin is achieved at 127.62 μg/mL Catechin. A dose- and time-dependent increase in the induction of apoptosis is observed when MCF-7 cells are treated with Catechin. When compare to the control cells at 24 hours, 40.7 and 41.16% of the cells treated with 150 μg/mL and 300 μg/mL Catechin, respectively, undergo apoptosis. The expression levels of Caspase-3, -8, and -9 and p53 in MCF-7 cells treated with 150 μg/mL Catechin for 24 h increase by 5.81, 1.42, 3.29, and 2.68 fold, respectively, as compare to the levels in untreated control cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Animals treated with Catechin at the lowest tested dose, i.e., 50 mg/kg, p.o. have spent comparatively more time in exploring the novel object in the choice trial, however, the difference is not statistically significant. Catechin prevents the time-induced episodic memory deficits in a dose-dependent manner, the most effective being 200 mg/kg, p.o.. Treatment with Catechin prevents the rise in MPO level compare to DOX alone treatment group (21.98±9.44 and 36.76±4.39% in the hippocampus and the frontal cortex respectively)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

308.28

Formula

C₁₅H₁₆O₇

CAS No.

88191-48-4

SMILES

O[C@@H]1[C@@H](C2=CC(O)=C(O)C=C2)OC3=CC(O)=CC(O)=C3C1.O

Structure Classification

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Waffo-Téguo P, et al. Potential cancer-chemopreventive activities of wine stilbenoids and flavans extracted from grape (Vitis vinifera) cell cultures. Nutr Cancer. 2001;40(2):173-9. [Content Brief]

[2]. Alshatwi AA. Catechin hydrate suppresses MCF-7 proliferation through TP53/Caspase-mediated apoptosis. J Exp Clin Cancer Res. 2010 Dec 17;29:167. [Content Brief]

[3]. Cheruku SP, et al. Catechin ameliorates doxorubicin-induced neuronal cytotoxicity in in vitro and episodic memory deficit in in vivo in Wistar rats. Cytotechnology. 2018 Feb;70(1):245-259. [Content Brief]

Cell Assay ^[2]	The Cell viability assay is performed to assess the toxicity of different concentrations of Catechin on MCF-7 cells. Briefly, MCF-7 cells (2×10⁴ cells/well) are plated in 96-well plates and treated with 0 μg/mL Catechin and 160 μg/mL Catechin for 24 hours. Then, 40 μL of the Cell Titer Blue solution is directly added to the wells and incubated at 37°C for 6 hours. The fluorescence is recorded with a 560 nm/590 nm (excitation/emission) filter set using a microplate fluorescence reader, and the IC₅₀ is calculated. Quadruplet samples are run for each concentration of Catechin in three independent experiments^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[3]	Rats^[3] Twelve weeks old, healthy male rats weighing 200 to 230 g are used in this study. Rats are divided into four experimental groups (n=9 each) for one vehicle and three groups of Catechin (three doses). The doses of Catechin are prepared at 50, 100, 200 mg/kg in 0.25% w/v sodium carboxy methylcellulose (CMC) and administered orally for 7 days prior to and during the experimental trials. Episodic memory, the conscious memory of the past experiences is evaluated in this study^[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Waffo-Téguo P, et al. Potential cancer-chemopreventive activities of wine stilbenoids and flavans extracted from grape (Vitis vinifera) cell cultures. Nutr Cancer. 2001;40(2):173-9. [Content Brief] [2]. Alshatwi AA. Catechin hydrate suppresses MCF-7 proliferation through TP53/Caspase-mediated apoptosis. J Exp Clin Cancer Res. 2010 Dec 17;29:167. [Content Brief] [3]. Cheruku SP, et al. Catechin ameliorates doxorubicin-induced neuronal cytotoxicity in in vitro and episodic memory deficit in in vivo in Wistar rats. Cytotechnology. 2018 Feb;70(1):245-259. [Content Brief]

Catechin hydrate Related Classifications

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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
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The dilution calculator equation

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Catechin88191-48-4(+)-CatechinCianidanolCatechuic acidCOXCyclooxygenaseInhibitorinhibitorinhibit

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