1. Epigenetics
    PI3K/Akt/mTOR
  2. AMPK
  3. AMPK activator 4

AMPK activator 4 

Cat. No.: HY-131334 Purity: 99.42%
Handling Instructions

AMPK activator 4 is a potent AMPK activator without inhibition of mitochondrial complex I. AMPK activator 4 selectively activates AMPK in the muscle tissues. AMPK activator 4 dose-dependently improves glucose tolerance in normal mice, and significantly lowers fasting blood glucose level and ameliorates insulin resistance in db/db diabetic mice. Anti-hyperglycemic effect.

For research use only. We do not sell to patients.

AMPK activator 4 Chemical Structure

AMPK activator 4 Chemical Structure

CAS No. : 2493239-46-4

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply Now  
5 mg USD 250 In-stock
Estimated Time of Arrival: December 31
10 mg USD 450 In-stock
Estimated Time of Arrival: December 31
25 mg USD 850 In-stock
Estimated Time of Arrival: December 31
50 mg USD 1350 In-stock
Estimated Time of Arrival: December 31
100 mg USD 1950 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

AMPK activator 4 is a potent AMPK activator without inhibition of mitochondrial complex I. AMPK activator 4 selectively activates AMPK in the muscle tissues. AMPK activator 4 dose-dependently improves glucose tolerance in normal mice, and significantly lowers fasting blood glucose level and ameliorates insulin resistance in db/db diabetic mice. Anti-hyperglycemic effect[1].

In Vitro

AMPK activator 4 (compound B10) (0-20 μM; 12-24 hours) induces phosphorylation of AMPK and its downstream protein ACC[1].
AMPK activator 4-mediated AMPK phosphorylation requires LKB1[1].
AMPK activator 4 slight decreases the viability of HepG2 cells after 72 hours of treatment at 10-20 μM[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: C2C12 myotubes, HepG2 and HuH-7 cells
Concentration: 0-20 μM
Incubation Time: 12-24 hours
Result: Induced dose-dependently phosphorylation of ACC in C2C12 myotube cells, and the phosphorylation of ACC was highly consistent with the phosphorylation of AMPK. Increased the phosphorylation of AMPK and ACC in human hepatocarcinoma HepG2 and HuH-7 cells.
In Vivo

AMPK activator 4 (100 mg/kg; Intragastrical administration; daily for 9 weeks) shows anti-hyperglycemic effect[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: db/db mice (5-week old)[1]
Dosage: 100 mg/kg
Administration: Intragastrical administration; daily for 9 weeks
Result: Significantly lowered the fasting blood glucose in db/db mice (2-6 weeks). Significantly improved insulin resistance in db/db mice (for 9 weeks). The body weight and the serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) (two markers of liver injury) were not significantly affected.
Molecular Weight

420.89

Formula

C₂₄H₂₁ClN₂O₃

CAS No.

2493239-46-4

SMILES

CC(C)(OC1=CC=C(Cl)C=C1)C(NC2=CC=C(OCC3=CC=C(C#N)C=C3)C=C2)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (593.98 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3759 mL 11.8796 mL 23.7592 mL
5 mM 0.4752 mL 2.3759 mL 4.7518 mL
10 mM 0.2376 mL 1.1880 mL 2.3759 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.94 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

AMPK activator 4AMPK activator4AMPK activator-4AMPKAMP-activated protein kinasemitochondrialcomplexImuscletissuesdb/dbdiabeticmiceglucoseInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
AMPK activator 4
Cat. No.:
HY-131334
Quantity:
MCE Japan Authorized Agent: