AMPK activator 4
Based on 1 Customer Validation
AMPK activator 4 is a potent AMPK activator without inhibition of mitochondrial complex I. AMPK activator 4 selectively activates AMPK in the muscle tissues. AMPK activator 4 dose-dependently improves glucose tolerance in normal mice, and significantly lowers fasting blood glucose level and ameliorates insulin resistance in db/db diabetic mice. Anti-hyperglycemic effect.
For research use only. We do not sell to patients.
- Purity: 98.64%
- CAS No.: 2493239-46-4
- Formula: C24H21ClN2O3
- Molecular Weight:420.89
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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All AMPK Isoforms
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Biological Activity
AMPK activator 4 (compound B10) (0-20 μM; 12-24 hours) induces phosphorylation of AMPK and its downstream protein ACC[1].
AMPK activator 4-mediated AMPK phosphorylation requires LKB1[1].
AMPK activator 4 slight decreases the viability of HepG2 cells after 72 hours of treatment at 10-20 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:C2C12 myotubes, HepG2 and HuH-7 cells
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Concentration:0-20 μM
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Incubation Time:12-24 hours
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Result:Induced dose-dependently phosphorylation of ACC in C2C12 myotube cells, and the phosphorylation of ACC was highly consistent with the phosphorylation of AMPK. Increased the phosphorylation of AMPK and ACC in human hepatocarcinoma HepG2 and HuH-7 cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:db/db mice (5-week old)[1]
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Dosage:100 mg/kg
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Administration:Intragastrical administration; daily for 9 weeks
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Result:Significantly lowered the fasting blood glucose in db/db mice (2-6 weeks). Significantly improved insulin resistance in db/db mice (for 9 weeks). The body weight and the serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) (two markers of liver injury) were not significantly affected.
Chemical Information
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CAS No. 2493239-46-4
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Appearance Solid
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Molecular Weight 420.89
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Formula C24H21ClN2O3
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Color White to off-white
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SMILES
CC(C)(OC1=CC=C(Cl)C=C1)C(NC2=CC=C(OCC3=CC=C(C#N)C=C3)C=C2)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Solvent & Solubility
DMSO : 250 mg/mL (593.98 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3759 mL | 11.8796 mL | 23.7592 mL | 59.3979 mL |
| 5 mM | 0.4752 mL | 2.3759 mL | 4.7518 mL | 11.8796 mL | |
| 10 mM | 0.2376 mL | 1.1880 mL | 2.3759 mL | 5.9398 mL | |
| 15 mM | 0.1584 mL | 0.7920 mL | 1.5839 mL | 3.9599 mL | |
| 20 mM | 0.1188 mL | 0.5940 mL | 1.1880 mL | 2.9699 mL | |
| 25 mM | 0.0950 mL | 0.4752 mL | 0.9504 mL | 2.3759 mL | |
| 30 mM | 0.0792 mL | 0.3960 mL | 0.7920 mL | 1.9799 mL | |
| 40 mM | 0.0594 mL | 0.2970 mL | 0.5940 mL | 1.4849 mL | |
| 50 mM | 0.0475 mL | 0.2376 mL | 0.4752 mL | 1.1880 mL | |
| 60 mM | 0.0396 mL | 0.1980 mL | 0.3960 mL | 0.9900 mL | |
| 80 mM | 0.0297 mL | 0.1485 mL | 0.2970 mL | 0.7425 mL | |
| 100 mM | 0.0238 mL | 0.1188 mL | 0.2376 mL | 0.5940 mL |
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