1. Epigenetics
    PI3K/Akt/mTOR
  2. AMPK
  3. AMPK activator 4

AMPK activator 4 

Cat. No.: HY-131334
Handling Instructions

AMPK activator 4 is a potent AMPK activator without inhibition of mitochondrial complex I. AMPK activator 4 selectively activates AMPK in the muscle tissues. AMPK activator 4 dose-dependently improves glucose tolerance in normal mice, and significantly lowers fasting blood glucose level and ameliorates insulin resistance in db/db diabetic mice. Anti-hyperglycemic effect.

For research use only. We do not sell to patients.

AMPK activator 4 Chemical Structure

AMPK activator 4 Chemical Structure

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Description

AMPK activator 4 is a potent AMPK activator without inhibition of mitochondrial complex I. AMPK activator 4 selectively activates AMPK in the muscle tissues. AMPK activator 4 dose-dependently improves glucose tolerance in normal mice, and significantly lowers fasting blood glucose level and ameliorates insulin resistance in db/db diabetic mice. Anti-hyperglycemic effect[1].

In Vitro

AMPK activator 4 (compound B10) (0-20 μM; 12-24 hours) induces phosphorylation of AMPK and its downstream protein ACC[1].
AMPK activator 4-mediated AMPK phosphorylation requires LKB1[1].
AMPK activator 4 slight decreases the viability of HepG2 cells after 72 hours of treatment at 10-20 μM[1].

Western Blot Analysis[1]

Cell Line: C2C12 myotubes, HepG2 and HuH-7 cells
Concentration: 0-20 μM
Incubation Time: 12-24 hours
Result: Induced dose-dependently phosphorylation of ACC in C2C12 myotube cells, and the phosphorylation of ACC was highly consistent with the phosphorylation of AMPK. Increased the phosphorylation of AMPK and ACC in human hepatocarcinoma HepG2 and HuH-7 cells.
In Vivo

AMPK activator 4 (100 mg/kg; Intragastrical administration; daily for 9 weeks) shows anti-hyperglycemic effect[1].

Animal Model: db/db mice (5-week old)[1]
Dosage: 100 mg/kg
Administration: Intragastrical administration; daily for 9 weeks
Result: Significantly lowered the fasting blood glucose in db/db mice (2-6 weeks). Significantly improved insulin resistance in db/db mice (for 9 weeks). The body weight and the serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) (two markers of liver injury) were not significantly affected.
Molecular Weight

420.89

Formula

C₂₄H₂₁ClN₂O₃

SMILES

CC(C)(OC1=CC=C(Cl)C=C1)C(NC2=CC=C(OCC3=CC=C(C#N)C=C3)C=C2)=O

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Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

AMPK activator 4AMPK activator4AMPK activator-4AMPKAMP-activated protein kinasemitochondrialcomplexImuscletissuesdb/dbdiabeticmiceglucoseInhibitorinhibitorinhibit

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Product Name:
AMPK activator 4
Cat. No.:
HY-131334
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