Cat. No.: HY-151361

AMPK-IN-3

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

AMPK-IN-3 (compound 67) is a potent and selective AMPK inhibitor with IC₅₀s of 60.7, 107 and 3820 nM for AMPK (α2), AMPK (α1) and KDR, respectively. AMPK-IN-3 inhibits AMPK does not affect cell viability or cause significant cytotoxicity in K562 cells. AMPK-IN-3 can be used in study of cancer.

For research use only. We do not sell to patients.

Purity: 98.00%
CAS No.: 2417674-27-0
Formula: C₂₅H₃₃N₅O₃
Molecular Weight:451.56

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

AMPK-IN-3 Chemical Structure

CAS No.: 2417674-27-0

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

AMPK (α2)

60.7 nM (IC₅₀)

AMPK (α1)

107 nM (IC₅₀)

KDR

3820 nM (IC₅₀)

All AMPK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
K562	EC₅₀	20.45 μM Compound: 67	Cytotoxicity against human K562 cells assessed as reduction in cell viability incubated for 48 hrs by celltox-green assay Cytotoxicity against human K562 cells assessed as reduction in cell viability incubated for 48 hrs by celltox-green assay	[PMID: 32334266]

In Vitro

AMPK-IN-3 (100 nM) shows inhibition values for AMPK(α2), FLT1, JAK1 JH2-pseudokinase and AMPK(α1) for 64%, 43%, 41% and 29%, respectively^[1].
AMPK-IN-3 (0.195313, 0.78125, 3.125, 12.5, 50 μM; 2 h) decreases the level of p-ACC in K562 cells^[1].
AMPK-IN-3 (1-100 μM; 24, 48, 72 h) shows potent inhibition of cellular AMPK activity but not affect cell viability^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

K562 cells
Concentration:

0.195313, 0.78125, 3.125, 12.5, 50 µM
Incubation Time:

2 h
Result:

Decreased cellular levels of p-ACC(Ser79) in K562 cells.

Cell Viability Assay^[1]

Cell Line:

K562 cells
Concentration:

1-100 µM
Incubation Time:

24, 48, 72 h
Result:

Showed no measurable impact on cell viability in K562 cells cultured under hypoxic conditions for 72 hours.

Chemical Information

CAS No. 2417674-27-0
Appearance Solid
Molecular Weight 451.56
Formula C₂₅H₃₃N₅O₃
Color Yellow to brown
SMILES

O=C(/C(C1=C2)=C\C3=C(C(C(NCCN(CC)CC)=O)=C(N3)C)C)NC1=CC=C2CCC(N)=O
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (6)

All WBCell Imaging/StainingCell Proliferation/Viability Assay

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Journal Impact Factor

Nature

Postprandial lipid metabolism durably enhances T cell immunity. [Abstract]

2026 Jun;654(8120):1065-1075. PMID: 42056521
Cell Death Dis

Combined metformin and simvastatin therapy inhibits SREBP2 maturation and alters energy metabolism in glioma. [Abstract]

2024 Nov 9;15(11):809. PMID: 39521788

AMPK-IN-3 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Nov 9;15(11):809. [Abstract]

Western blot analysis of T98G cells treated with AMPK inhibitor (HY-151361) revealed alterations in GLUT1, GLUT6, and SREBP2 protein levels
iScience

Roquin-1 resolves sepsis-associated acute liver injury by regulating inflammatory profiles via miRNA cargo in extracellular vesicles. [Abstract]

2023 Jul 12;26(8):107295. PMID: 37554446
Sci Rep

DMH1 improves palmitic acid-Induced insulin resistance in cardiomyocytes via PP2A inhibition and AKT/AMPK signaling activation. [Abstract]

2026 Feb 13;16(1):8822. PMID: 41688642
Cell Signal

α-KG alleviates mitochondrial dysfunction and attenuates HPDLSCs senescence in periodontitis through LKB1-AMPK activation. [Abstract]

2026 Jul:143:112442. PMID: 41740638
Wound Repair Regen

Suppression of TRIM72-mediated endoplasmic reticulum stress facilitates FOXM1 promotion of diabetic ulcer healing. [Abstract]

2025 Jan-Feb;33(1):e13247. PMID: 39721954

AMPK-IN-3 purchased from MedChemExpress. Usage Cited in: Wound Repair Regen. 2025 Jan-Feb;33(1):e13247. [Abstract]

Representative transmission electron microscopy (TEM) images showing the ultrastructure of ER in fibroblasts. FOXM1‐transfected fibroblasts were pretreated with AMPK‐IN‐3 (100 nM) for 2 h before exposure to HG.

AMPK-IN-3 purchased from MedChemExpress. Usage Cited in: Wound Repair Regen. 2025 Jan-Feb;33(1):e13247. [Abstract]

AMPK‐IN‐3 (100 nM) for 2 h. Western blotting analysis of AMPKα, p‐AMPKα, and GRP78.

AMPK-IN-3 purchased from MedChemExpress. Usage Cited in: Wound Repair Regen. 2025 Jan-Feb;33(1):e13247. [Abstract]

FOXM1‐transfected fibroblasts were pretreated with AMPK‐IN‐3 (100 nM) for 2 h before exposure to HG. EdU assay measuring cell proliferation.

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (221.45 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.2145 mL

11.0727 mL

22.1455 mL

5 mM

0.4429 mL

2.2145 mL

4.4291 mL

10 mM

0.2215 mL

1.1073 mL

2.2145 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Matheson CJ, et al. Substituted oxindol-3-ylidenes as AMP-activated protein kinase (AMPK) inhibitors. Eur J Med Chem. 2020 Jul 1;197:112316. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.2145 mL	11.0727 mL	22.1455 mL	55.3636 mL
	5 mM	0.4429 mL	2.2145 mL	4.4291 mL	11.0727 mL
	10 mM	0.2215 mL	1.1073 mL	2.2145 mL	5.5364 mL
	15 mM	0.1476 mL	0.7382 mL	1.4764 mL	3.6909 mL
	20 mM	0.1107 mL	0.5536 mL	1.1073 mL	2.7682 mL
	25 mM	0.0886 mL	0.4429 mL	0.8858 mL	2.2145 mL
	30 mM	0.0738 mL	0.3691 mL	0.7382 mL	1.8455 mL
	40 mM	0.0554 mL	0.2768 mL	0.5536 mL	1.3841 mL
	50 mM	0.0443 mL	0.2215 mL	0.4429 mL	1.1073 mL
	60 mM	0.0369 mL	0.1845 mL	0.3691 mL	0.9227 mL
	80 mM	0.0277 mL	0.1384 mL	0.2768 mL	0.6920 mL
	100 mM	0.0221 mL	0.1107 mL	0.2215 mL	0.5536 mL

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AMPK-IN-3

Epigenetics Compound Library

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

PI3K/Akt/mTOR Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Covalent Screening Library

Antioxidant Compound Library

Oxygen Sensing Compound Library

Glycolysis Compound Library

Cytoskeleton Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Lipid Metabolism Compound Library

Glucose Metabolism Compound Library

Cancer Stem Cells Compound Library

Pain-Related Compound Library

Highly Selective Inhibitors Library

Serine/Threonine Kinase Inhibitor Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

Kinase Inhibitor Library Mini

MCE Bioactive Compound Library

Covalent Screening Library Plus

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Lactic Acid Metabolic Compound Library

High-Throughput Bioactive Compound Library

Posttranslational Modification Library

Publications Citing Use of MedChemExpress (MCE) AMPK-IN-3

All AMPK Isoforms

Biological Activity

Chemical Information

Publications (6)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

AMPK-IN-3 Related Classifications

HY-151361 Related Classifications