Anti-inflammatory agent 64
Anti-inflammatory agent 64 (compound 4b) inhibits the secretion of IL-6 and TNF-α. Anti-inflammatory agent 64 has antioxidant and anti-inflammatory activity in vitro and in vivo. Anti-inflammatory agent 64 can effectively reduce paw edema .
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- No. CAS: 3016401-76-3
- Fòrmula: C20H21ClN2O3
- Peso molecular:372.85
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Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| RAW264.7 | IC50 |
0.9 μM
Compound: 4b
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Antiinflammatory activity in LPS-induced mouse RAW264.7 cells assessed as inhibition of NO production pretreated for 1 hr followed by LPS addition and measured after 24 hrs by griess reagent based assay
Antiinflammatory activity in LPS-induced mouse RAW264.7 cells assessed as inhibition of NO production pretreated for 1 hr followed by LPS addition and measured after 24 hrs by griess reagent based assay
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[PMID: 37976711] |
| RAW264.7 | IC50 |
1.52 μM
Compound: 4b
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Antiinflammatory activity in LPS-induced mouse RAW264.7 cells assessed as reduction in TNF-alpha level incubated for 24 hrs by ELISA
Antiinflammatory activity in LPS-induced mouse RAW264.7 cells assessed as reduction in TNF-alpha level incubated for 24 hrs by ELISA
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[PMID: 37976711] |
| RAW264.7 | IC50 |
1.73 μM
Compound: 4b
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Antiinflammatory activity in LPS-induced mouse RAW264.7 cells assessed as reduction in IL-6 level incubated for 24 hrs by ELISA
Antiinflammatory activity in LPS-induced mouse RAW264.7 cells assessed as reduction in IL-6 level incubated for 24 hrs by ELISA
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[PMID: 37976711] |
In Vitro
Anti-inflammatory agent 64 (2 μM, 4 h) can significantly protect cells and promote cell proliferation in RAW264.7 cells stimulated by H2O2 (0.4 mM[1].
Anti-inflammatory agent 64 (2 μM, 24 h) inhibits the production of NO (IC50=0.9 μM) in RAW264.7 cells stimulated by LPS (1 μg/mL) and has anti-inflammatory effect[1].
Anti-inflammatory agent 64 (0.125-2 μM, 4-24 h) significantly reduces the protein level of ROS and enhances HO-1 protein level in RAW264.7 cells stimulated by Rot (5 μM) and LPS (1 μM/mL) and has antioxidant effect[1].
Anti-inflammatory agent 64 (0.5-2 μM, 24 h) can restrain IL–6 in dose dependent manner (IC50=1.73 μM) and TNF-α (IC50=1.52 μM)[1].
Anti-inflammatory agent 64 (0.5-2 μM) can inhibit the expresstion of iNOS protein[1].
Anti-inflammatory agent 64 (0.5-2 μM) inhibits the avtivition of NF-κB pathway by inhibiting the degradation and phosphorylation of IκBα in RAW264.7 cells stimulated by LPS (1 μg/mL)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:RAW264.7
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Concentration:2 μM
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Incubation Time:4 h
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Result:Could effectively protect the cells treated with LPS, and the cell survival rate is 85%. Could effectively inhibit inflammatory factors ON and had anti-inflammatory properties.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Cg-induced mouse paw edema model[1]
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Dosage:1 mg/kg, 10 mg/kg, 30 mg/kg
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Administration:Intraperitoneal injection (i.p.) and intragastrica (i.g.), single dose
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Result:Could significantly inhibit plantar edema Reduced the expression level of iNOS. Decreased the level of oxidation marker MAD and increased the activity of antioxidant enzyme SOD. Increased HO-1 expression in a dose-dependent manner.
Chemical Information
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No. CAS 3016401-76-3
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Peso molecular 372.85
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Fòrmula C20H21ClN2O3
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SMILES
O=C(NCCCN1CCC2=C1C=CC(Cl)=C2)/C=C/C3=CC(O)=C(O)C=C3
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-inflammatory agent 64 Related Classifications
HY-155405 Related Classifications
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