Antibacterial agent 327 

Antibacterial agent 327 (Compound 6f) is an Antibacterial agent. Antibacterial agent 327 potently inhibits the supercoiling activity of Staphylococcus aureus DNA gyrase with an IC₅₀ of 0.28 μM. It also inhibits the ATPase activities of DNA gyrase and Topoisomerase IV, as well as the decatenation activity of Topoisomerase IV (IC₅₀: 0.43 μM, 0.73 μM, and 2.31 μM, respectively). Antibacterial agent 327 potently inhibits Clostridioides difficile and methicillin-resistant Staphylococcus aureus (HY-121544), with an MIC of 0.78 μg/mL for both. Antibacterial agent 327 inhibits Escherichia coli with an MIC₅₀ of 0.78 μg/mL^[1].

Antibacterial agent 327 potently inhibits Clostridium difficile and Methicillin (HY-121544)-resistant Staphylococcus aureus, with an MIC of 0.78 μg/mL for both^[1].
Antibacterial agent 327 (0.78-100 μg/mL) potently inhibits Escherichia coli, with an MIC₅₀ of 0.78 μg/mL; meanwhile, it shows weak activity against Pseudomonas aeruginosa and Salmonella typhimurium, and limited activity against Klebsiella pneumoniae^[1].
Antibacterial agent 327 (0.28-2.31 μM) potently inhibits the supercoiling activity of Staphylococcus aureus DNA gyrase with an IC₅₀ of 0.28 μM. It also inhibits the ATPase activities of DNA gyrase and topoisomerase IV, as well as the decatenation activity of topoisomerase IV^[1].
Antibacterial agent 327 exhibits an IC₅₀ value of 85.52 μM against WI-38 fibroblasts^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

832.23

C₃₄H₄₂ClN₃O₁₇S

1311163-39-9

CC(O[C@H]([C@H]([C@@H](O[C@@H]1COC(C)=O)NC(N/N=C/C2=CC=C(C=C2)Cl)=S)OC(C)=O)[C@@H]1O[C@@H]3[C@@H]([C@H]([C@@H]([C@H](O3)COC(C)=O)OC(C)=O)OC(C)=O)OC(C)=O)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Van HTK, et al. Maltose-Conjugated Thiosemicarbazones from Substituted Benzaldehydes: Synthesis and Inhibitory Activity against Gram-(+) and Gram-(-) Bacteria. ACS Med Chem Lett. 2026;17(3):670-678. Published 2026 Feb 11.  [Content Brief]