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  4. Encephalomyelitis Models
  5. AQP4 (201-220)

AQP4 (201-220) is an encephalitogenic epitope of AQP-4. AQP4 (201-220) can induce experimental autoimmune encephalomyelitis (EAE) (characterized by midline lesions in the brain, retinal pathology, and lesions at the grey matter/white matter border zone in the spinal cord). AQP-4 is a target antigen in neuromyelitis optica.

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AQP4 (201-220)

AQP4 (201-220) Chemical Structure

CAS No. : 2104700-59-4

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Description

AQP4 (201-220) is an encephalitogenic epitope of AQP-4. AQP4 (201-220) can induce experimental autoimmune encephalomyelitis (EAE) (characterized by midline lesions in the brain, retinal pathology, and lesions at the grey matter/white matter border zone in the spinal cord). AQP-4 is a target antigen in neuromyelitis optica[1].

In Vivo

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

AQP4 (201-220) can be used to induce experimental autoimmune encephalomyelitis (EAE) models[1][2][3][4].

Induction of experimental autoimmune encephalomyelitis[1][2][3][4]
Background
AQP4 (201-220) breaks through immune tolerance and activates specific T cells. The AQP4 (201-220)-specific T cells directionally migrate to the regions with high AQP4 expression in the central nervous system (CNS), directly kill AQP4+ astrocytes or activate glial cells, thereby triggering autoimmune inflammatory damage.
Specific Modeling Methods
1. Mice: C57BL/6J, Aqp4⁻/⁻ mice
Administration: AQP4 (201-220) 200 μg • s.c. (the base of the mouse's tail) • single dose
Note
(1) AQP4 (201-220) needs to be emulsified with Complete Freund's adjuvant (CFA) (HY-153808) containing 250/500 μg of Mycobacterium tuberculosis H37Ra into a 200 μL emulsion for administration.
(2) At the same time as the administration, pertussis toxin (200 ng per mouse) is injected intravenously, and another dose is given on the second day.
Modeling Indicators
Clinical score: 0 point: No symptoms of any disease; 1 point: Loss of tail tension (tail drooping, no voluntary movement); 2 points: Impaired ability to turn over (unable to voluntarily turn from supine to prone position); 3 points: Paraplegia of both hind limbs (hind limbs unable to support body weight, dragging while walking); 4 points: Quadriplegia (loss of motor function in both forelimbs and hind limbs); 5 points: Moribund state (no voluntary activity, weak breathing). Clinical symptoms start to appear around day 11 after immunization (mostly 1 or 2 points), reach the peak around day 18 (scores are mostly 2-3 points), and enter the recovery period on day 30 (scores decrease); if the clinical score is ≥1 point, it indicates successful modeling.
Histological analysis: Scattered or focal inflammatory lesions appear in the gray-white matter junction area; inflammatory lesions appear in the diencephalic midline area (near the third ventricle) and around the fourth ventricle; inflammatory infiltration appears in the inner layer of the retina, accompanied by loss of retinal ganglion cells.
Cellular analysis: Significant infiltration of CD45+ immune cells and CD4+ T cells in the CNS (brain, spinal cord, retina), and an increase in the number of Iba-1 and GAFP positive cells in the lesion area.
Molecular changes: Decreased expression of AQP4; increased expression of IFN-γ, IL-17, CXCL10, and CCL20.

Correlated Product(s): Aquaporin 4 Antibody (HY-P83459A); Complete Freund's adjuvant (CFA) (HY-153808); Anti-Mouse IFN gamma Antibody (XMG1.2) (HY-P990798)
Opposite Product(s): Anti-Mouse IL-6R Antibody (15A7) (HY-P990211); Anti-Mouse/Rat IL-17A Antibody (17F3) (HY-P990222)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

2151.40

Formula

C97H143N27O27S
CAS No.
Sequence

His-Leu-Phe-Ala-Ile-Asn-Tyr-Thr-Gly-Ala-Ser-Met-Asn-Pro-Ala-Arg-Ser-Phe-Gly-Pro
Sequence Shortening

HLFAINYTGASMNPARSFGP
SMILES

O=C(N[C@@H](CC(C)C)C(N[C@@H](CC1=CC=CC=C1)C(N[C@@H](C)C(N[C@@H]([C@@H](C)CC)C(N[C@@H](CC(N)=O)C(N[C@@H](CC2=CC=C(C=C2)O)C(N[C@@H]([C@H](O)C)C(NCC(N[C@@H](C)C(N[C@@H](CO)C(N[C@@H](CCSC)C(N[C@@H](CC(N)=O)C(N3[C@@H](CCC3)C(N[C@@H](C)C(N[C@@H](CCCNC(N)=N)C(N[C@@H](CO)C(N[C@@H](CC4=CC=CC=C4)C(NCC(N5[C@@H](CCC5)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)=O)[C@H](CC6=CNC=N6)N
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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AQP4 (201-220) Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

AQP4 (201-220)2104700-59-4AquaporinAQPEAEneuromyelitis opticaexperimental autoimmune encephalomyelitisInhibitorinhibitorinhibit

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