Art. -Nr.: HY-B0368

Captopril

(Synonyms: SQ 14225)

23 Cited Publications

Kundenbewertung

Based on 23 publication(s) in Google Scholar

Captopril (SQ 14225), antihypertensive agent, is a thiol-containing competitive, orally active angiotensin-converting enzyme (ACE) inhibitor (IC₅₀=0.025 μM) and has been widely used for research of hypertension and congestive heart failure. Captopril is also a New Delhi metallo-β-lactamase-1 (NDM-1) inhibitor with an IC₅₀ of 7.9 μM.

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Reinheit: 99.84%
CAS. Nr.: 62571-86-2
Formel: C₉H₁₅NO₃S
Molecular Weight:217.29

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Captopril Chemical Structure

CAS. Nr.: 62571-86-2

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- Clinical Trial
Chemical Information
Publications (23)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

ACE^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
HeLa	EC₅₀	130 μM Compound: L-A1	Cytotoxicity against human HeLa cells incubated for 72 hrs by alamar blue cell viability assay Cytotoxicity against human HeLa cells incubated for 72 hrs by alamar blue cell viability assay	[PMID: 39013490]
KB 3-1	IC₅₀	15.67 mM Compound: captropril	Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay Cytotoxicity against human KB-3-1 cells incubated for 72 hrs by MTT assay	[PMID: 21657271]
KB-V1	IC₅₀	13.07 mM Compound: captropril	Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay Cytotoxicity against drug-resistant human KBV1 cells expressing P-gp incubated for 72 hrs by MTT assay	[PMID: 21657271]

In Vitro

Captopril (SQ 14225) has been shown to have similar morbidity and mortality benefits to those of diuretics and beta-blockers in hypertensive patients. Captopril has been shown to delay the progression of diabetic nephropathy, and enalapril and lisinopril prevent the development of nephropathy in normoalbuminuric patients with diabetes^[4].
An equimolar ratio of the cis and trans states of Captopril exists in solution and that the enzyme selects only the trans state of the inhibitor that presents architectural and stereoelectronic complementarity with its substrate binding groove^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 62571-86-2
Appearance Solid
Molecular Weight 217.29
Formel C₉H₁₅NO₃S
Color White to yellow
SMILES

O=C(O)[C@H]1N(C([C@H](C)CS)=O)CCC1
Synonyms

SQ 14225
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (23)

All In Vivo Efficacy StudyHistological Imaging/Staining

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Journal Impact Factor

Nat Commun

Reduced hepatic bradykinin degradation accounts for cold-induced BAT thermogenesis and WAT browning in male mice. [Abstract]

2023 May 2;14(1):2523. PMID: 37130842

Captopril purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 May 2;14(1):2523. [Abstract]

Serum BK levels from sWAT from WT male mice treated with 40 mg/kg/day Captopril or 70 mg/kg/day Enalapril by gavage once a day for 21 days. The results showed that Captopril significantly increased serum BK levels.

Captopril purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 May 2;14(1):2523. [Abstract]

Representative immunohistochemistry (IHC) staining of UCP1 and H&E staining of sWAT. Captopril (40 mg/kg; once daily; 21 days; 25°C) induced sWAT brownin.

Captopril purchased from MedChemExpress. Usage Cited in: Nat Commun. 2023 May 2;14(1):2523. [Abstract]

Captopril (40 mg/kg; once daily; 21 days; 25°C) decreased the weight of sWAT in WT male mice.
Allergy

Long-Term Attenuation of Vascular Hyperpermeability in a Hereditary Angioedema Mouse Model by Adenine Base Editing. [Abstract]

2025 Nov 14. PMID: 41236489

Captopril purchased from MedChemExpress. Usage Cited in: Allergy. 2025 Nov 14. [Abstract]

Representative images of feet collected 30 min post-Evans Blue (EB) injection. Mice were assigned to five groups: WT control, Serping1^+/− control, WT + Captopril (2.5 mg/kg; i.v.), Serping1^+/− + Captopril + PBS, and Serping1^+/− + Captopril + AAV-NG-ABE8e. Captopril elicited significant Evans blue leakage.

Captopril purchased from MedChemExpress. Usage Cited in: Allergy. 2025 Nov 14. [Abstract]

Representative intravital confocal images showing real-time FITC-dextran extravasation from intestinal microvasculature. Mice were assigned to: WT control, Serping1^+/− control, WT + Captopril (2.5 mg/kg; i.v.), Serping1^+/− + Captopril + PBS, and Serping1^+/− + Captopril + AAV-NG-ABE8e. By 30 min, Captopril-treated mice demonstrated pronounced FITC-dextran extravasation—both in area and fluorescence intensity—relative to WT and Serping1^+/− controls.
Phytomedicine

Shexiang baoxin pill ameliorates cardiac fibrosis by inhibiting fibroblast to myofibroblast transition through STAT3 phosphorylation suppression. [Abstract]

2025 Sep 15:148:157263. PMID: 40975042
Phytomedicine

Quercitrin improves cardiac remodeling following myocardial infarction by regulating macrophage polarization and metabolic reprogramming. [Abstract]

2024 May:127:155467. PMID: 38447360
Phytomedicine

Ethyl ferulate suppresses post-myocardial infarction myocardial fibrosis by inhibiting transforming growth factor receptor 1. [Abstract]

2023 Dec:121:155118. PMID: 37801895
ACS Environ Au

Machine Learning-Assisted Recognition of Environmental Sulfur-Containing Chemicals in Nontargeted Mass Spectrometry Analysis of Inadequate Mass Resolution. [Abstract]

2025 Aug 5;5(6):573-582. PMID: 41277996
Apoptosis

Targeting PAX8 sensitizes ovarian cancer cells to ferroptosis by inhibiting glutathione synthesis. [Abstract]

2024 Oct;29(9-10):1499-1514. PMID: 38853202
Anal Chem

Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]

2025 Jun 3;97(21):11099-11109. PMID: 40401576
Eur J Med Chem

Development of targeted nanoparticles loaded with antiviral drugs for SARS-CoV-2 inhibition. [Abstract]

2022 Mar 5:231:114121. PMID: 35114539
Cell Rep Methods

RECOVER identifies synergistic drug combinations in vitro through sequential model optimization. [Abstract]

2023 Oct 23;3(10):100599. PMID: 37797618
Eur J Pharmacol

Captopril alleviates radiation-induced pulmonary fibrosis by suppressing PAI-1 expression and cytoskeleton-dependent epithelial-to-mesenchymal transition. [Abstract]

2025 Aug 6:1005:178045. PMID: 40780595
Mar Drugs

Affinity Purification and Molecular Characterization of Angiotensin-Converting Enzyme (ACE)-Inhibitory Peptides from Takifugu flavidus. [Abstract]

2023 Sep 29;21(10):522. PMID: 37888457
Eur J Pharmacol

Montelukast, cysteinyl leukotriene receptor 1 antagonist, inhibits cardiac fibrosis by activating APJ. [Abstract]

2022 May 15;923:174892. PMID: 35358494
Eur J Pharmacol

Captopril alleviates glucocorticoid-induced osteonecrosis of the femoral head by mediating the ACE2/ Ang-(1-7)/Mas receptor cascade. [Abstract]

2022 Apr 15:921:174871. PMID: 35283112
Sci Rep

Effect of captopril on post-infarction remodelling visualized by light sheet microscopy and echocardiography. [Abstract]

2021 Mar 4;11(1):5241. PMID: 33664407
J Cell Mol Med

Pirfenidone alleviates cardiac fibrosis induced by pressure overload via inhibiting TGF-β1/Smad3 signalling pathway. [Abstract]

2022 Aug;26(16):4548-4555. PMID: 35861038
Am J Physiol Heart Circ Physiol

Overexpression of the neuronal human (pro)renin receptor mediates angiotensin II-independent blood pressure regulation in the central nervous system. [Abstract]

2018 Mar 1;314(3):H580-H592. PMID: 29350998
J Orthop Surg Res

Several first-line anti-hypertensives act on fibrosarcoma progression and PD1ab blockade therapy. [Abstract]

2024 Feb 19;19(1):147. PMID: 38373964
Front Oncol

The Angiotensin-Converting Enzyme Inhibitory State Promotes the Transformation of Non-Small Cell Lung Cancer Blood Supply Pattern Toward Vasculogenic Mimicry Formation. [Abstract]

2021 Jun 16;11:663671. PMID: 34221978
Arch Physiol Biochem

Huaiqihuang (HQH) protects podocytes from high glucose-induced apoptosis and inflammation response by regulating PI3K/AKT/mTOR pathway. [Abstract]

2025 Apr;131(2):285-292. PMID: 39329410
Medicine

Mechanism of Huaiqihuang in treatment of diabetic kidney disease based on network pharmacology, molecular docking and in vitro experiment. [Abstract]

2023 Dec 15;102(50):e36177. PMID: 38115276
bioRxiv

2026 Jun 19.
Biomed Pharmacother

Caffeic acid mitigates myocardial fibrosis and improves heart function in post-myocardial infarction by inhibiting transforming growth factor-β receptor 1 signaling pathways. [Abstract]

2024 Jun 20:177:117012. PMID: 38906025

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : ≥ 50 mg/mL (230.11 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : ≥ 50 mg/mL (230.11 mM)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.6021 mL

23.0107 mL

46.0214 mL

5 mM

0.9204 mL

4.6021 mL

9.2043 mL

10 mM

0.4602 mL

2.3011 mL

4.6021 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (11.51 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (11.51 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (11.51 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 32.5 mg/mL (149.57 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Afrin S, et al. Eritadenine from Edible Mushrooms Inhibits Activity of Angiotensin Converting Enzyme in Vitro. J Agric Food Chem. 2016;64(11):2263-2268. [Content Brief]

[2]. Esmaeili S, et al. Captopril/enalapril inhibit promiscuous esterase activity of carbonic anhydrase at micromolar concentrations: An in vitro study. Chem Biol Interact. 2017;265:24-35. [Content Brief]

[3]. Li N, et al. Simplified captopril analogues as NDM-1 inhibitors. Bioorg Med Chem Lett. 2014;24(1):386-389. [Content Brief]

[4]. Tzakos, A.G., et al., The molecular basis for the selection of captopril cis and trans conformations by angiotensin I converting enzyme. Bioorg Med Chem Lett, 2006. 16(19): p. 5084-7. [Content Brief]

[5]. Song, J.C. and C.M. White, Clinical pharmacokinetics and selective pharmacodynamics of new angiotensin converting enzyme inhibitors: an update. Clin Pharmacokinet, 2002. 41(3): p. 207-24. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	4.6021 mL	23.0107 mL	46.0214 mL	115.0536 mL
	5 mM	0.9204 mL	4.6021 mL	9.2043 mL	23.0107 mL
	10 mM	0.4602 mL	2.3011 mL	4.6021 mL	11.5054 mL
	15 mM	0.3068 mL	1.5340 mL	3.0681 mL	7.6702 mL
	20 mM	0.2301 mL	1.1505 mL	2.3011 mL	5.7527 mL
	25 mM	0.1841 mL	0.9204 mL	1.8409 mL	4.6021 mL
	30 mM	0.1534 mL	0.7670 mL	1.5340 mL	3.8351 mL
	40 mM	0.1151 mL	0.5753 mL	1.1505 mL	2.8763 mL
	50 mM	0.0920 mL	0.4602 mL	0.9204 mL	2.3011 mL
	60 mM	0.0767 mL	0.3835 mL	0.7670 mL	1.9176 mL
	80 mM	0.0575 mL	0.2876 mL	0.5753 mL	1.4382 mL
	100 mM	0.0460 mL	0.2301 mL	0.4602 mL	1.1505 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Captopril

23 Cited Publications

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Captopril

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Publications Citing Use of MedChemExpress (MCE) Captopril

Biologische Aktivität

Chemical Information

Publications (23)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Captopril Related Classifications

HY-B0368 Related Classifications