Art. -Nr.: HY-13915

NSC348884

4 Cited Publications

Kundenbewertung

Based on 4 publication(s) in Google Scholar

NSC348884 is a nucleophosmin (NPM) inhibitor, it disrupts oligomer formation and induces apoptosis, inhibits cell proliferation with IC₅₀s of 1.7-4.0 μM in distinct cancer cell lines. NSC348884 can be used for the research of cancer.

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Reinheit: 99.40%
CAS. Nr.: 81624-55-7
Formel: C₃₈H₄₀N₁₀
Molecular Weight:636.79

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

NSC348884 Chemical Structure

CAS. Nr.: 81624-55-7

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

IC50: 4 μM (LNCaP cells), 1.7 μM (Granta cells)^[1]

In Vitro

NSC348884 (0-6 μM; 24 h) binds with NPM and disrupts NPM oligomer formation^[1].
NSC348884 (0.01-100 μM; 4 d) shows cytotoxicity to cancer cells^[1].
NSC348884 (1 μM; 4 d) shows cytotoxicity to mantle cell lymphoma with 0.1 nM doxorubicin^[1].
NSC348884 (1-5 μM; 24 h) induces cell apoptosis^[1].
NSC348884 (0-5 μM; 0-24 h) upregulates the concentration of p53^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

LNCaP and Granta cell lines
Concentration:

0.01-100 μM
Incubation Time:

4 days
Result:

Inhibited LNCaP cells and Granta cell lines with IC₅₀s of 4 and 1.7 μM, respectively.

Apoptosis Analysis^[1]

Cell Line:

LNCaP cell line
Concentration:

1-5 μM
Incubation Time:

24 h
Result:

Induced apoptosis in LNCaP cell line.

Western Blot Analysis^[1]

Cell Line:

LNCaP cell line
Concentration:

0-5 μM
Incubation Time:

0-24 h
Result:

Dose-dependently increased both p53 protein level and phosphorylation on Ser15, and increased the concentration of p21.

Western Blot Analysis^[2]

Cell Line:

SKOV3, ES2 and MOSEC/Luc cell lines
Concentration:

0, 2.5 and 5 μM
Incubation Time:

48 h
Result:

Increased PD-L1 expression in ovarian cancer cells.

In Vivo

NSC348884 (50 μg; i.p. once) reduces tumor growth^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57Bl/6J mice with MOSEC/Luc cells injection^[2]
Dosage:

50 μg
Administration:

Intraperitoneal injection; 50 μg once
Result:

Reduced tumorigenesis, but not completely inhibited.

Chemical Information

CAS. Nr. 81624-55-7
Appearance Solid
Molecular Weight 636.79
Formel C₃₈H₄₀N₁₀
Color Light yellow to yellow
SMILES

CC1=CC=C2N=C(CN(CC3=NC4=CC=C(C)C=C4N3)CCN(CC5=NC6=CC=C(C)C=C6N5)CC7=NC8=CC=C(C)C=C8N7)NC2=C1
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (4)

All WBIFCell Proliferation/Viability AssayRT-PCR

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Journal Impact Factor

J Hematol Oncol

NPM1 inhibits tumoral antigen presentation to promote immune evasion and tumor progression. [Abstract]

2024 Oct 14;17(1):97. PMID: 39402629
Front Microbiol

Nucleolar Phosphoprotein NPM1 Interacts With Porcine Circovirus Type 3 Cap Protein and Facilitates Viral Replication. [Abstract]

2021 May 25:12:679341. PMID: 34113334

NSC348884 purchased from MedChemExpress. Usage Cited in: Front Microbiol. 2021 May 25:12:679341. [Abstract]

NPM1 inhibitor NSC348884 (0.1, 1 μM) and DMSO-treated cells were infected with PCV3 for 48, 72, and 96 h. Western blot was used to examine the expression levels of Cap, NPM1, and β-actin

NSC348884 purchased from MedChemExpress. Usage Cited in: Front Microbiol. 2021 May 25:12:679341. [Abstract]

NPM1 expression in NSC348884 (1 μM)- and DMSO-treated cells analyzed by immunofluorescence.

NSC348884 purchased from MedChemExpress. Usage Cited in: Front Microbiol. 2021 May 25:12:679341. [Abstract]

Viability of PK15 cells treated with NSC348884 (0.1, 1 μM) and DMSO analyzed using a CCK-8 assay.

NSC348884 purchased from MedChemExpress. Usage Cited in: Front Microbiol. 2021 May 25:12:679341. [Abstract]

qPCR was exploited to determine the expression level of Cap gene treated with NSC348884.
Res Sq

2026 Apr 21.
Eur Rev Med Pharmacol Sci

The effect of small molecule inhibitor NSC348884 on nucleophosmin 1-mutated acute myeloid leukemia cells. [Abstract]

2023 Oct;27(19):9145-9151. PMID: 37843328

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : ≥ 34 mg/mL (53.39 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.5704 mL

7.8519 mL

15.7038 mL

5 mM

0.3141 mL

1.5704 mL

3.1408 mL

10 mM

0.1570 mL

0.7852 mL

1.5704 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.5 mg/mL (3.93 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (3.93 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (3.93 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (392 KB)
- English - EN (392 KB)
- Français - FR (392 KB)
- Deutsch - DE (392 KB)
- Norwegian - NO (392 KB)
- Español - ES (392 KB)
- Swedish - SV (392 KB)
- Italian - IT (392 KB)
- Korean - KR (392 KB)
- Portuguese - PT (392 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Qi W, et al. NSC348884, a nucleophosmin inhibitor disrupts oligomer formation and induces apoptosis in human cancercells. Oncogene. 2008 Jul 10;27(30):4210-20. [Content Brief]

[2]. Lin Y, et al. Inhibition of nucleophosmin/B23 sensitizes ovarian cancer cells to antiprogrammed cell death protein-1 ligand therapy. 2022.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.5704 mL	7.8519 mL	15.7038 mL	39.2594 mL
	5 mM	0.3141 mL	1.5704 mL	3.1408 mL	7.8519 mL
	10 mM	0.1570 mL	0.7852 mL	1.5704 mL	3.9259 mL
	15 mM	0.1047 mL	0.5235 mL	1.0469 mL	2.6173 mL
	20 mM	0.0785 mL	0.3926 mL	0.7852 mL	1.9630 mL
	25 mM	0.0628 mL	0.3141 mL	0.6282 mL	1.5704 mL
	30 mM	0.0523 mL	0.2617 mL	0.5235 mL	1.3086 mL
	40 mM	0.0393 mL	0.1963 mL	0.3926 mL	0.9815 mL
	50 mM	0.0314 mL	0.1570 mL	0.3141 mL	0.7852 mL

Keywords

NSC348884

4 Cited Publications

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NSC348884

Apoptosis Compound Library

Anti-Cancer Compound Library

Anti-Blood Cancer Compound Library

Cell Death Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) NSC348884

Biologische Aktivität

Chemical Information

Publications (4)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

NSC348884

Apoptosis Compound Library

Anti-Cancer Compound Library

Anti-Blood Cancer Compound Library

Cell Death Library

Anti-Hematopathy Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) NSC348884

Biologische Aktivität

Chemical Information

Publications (4)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

NSC348884 Related Classifications

HY-13915 Related Classifications