Art. -Nr.: HY-N0796

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Procyanidin B2

(Synonyms: Proanthocyanidin B2)

17 Cited Publications

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Based on 17 publication(s) in Google Scholar

Procyanidin B2 is a natural flavonoid, with anti-cancer, antioxidant activities.

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Reinheit: 99.89%
CAS. Nr.: 29106-49-8
Formel: C₃₀H₂₆O₁₂
Molecular Weight:578.52

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Procyanidin B2 Chemical Structure

CAS. Nr.: 29106-49-8

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (17)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll
Reinheit & Dokumentation
Verweise
Help & FAQs
Bioz

Biologische Aktivität

Beschreibung

Procyanidin B2 is a natural flavonoid, with anti-cancer, antioxidant activities.

Cellular Effect

Cell Line	Type	Value	Description	References
A549	ED₅₀	>10 μg/mL Compound: 48	Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human A549 cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]
HCT-8	ED₅₀	>10 μg/mL Compound: 48	Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human HCT8 cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]
HUVEC	IC₅₀	3.8 μM Compound: 44	Inhibition of NADPH oxidase in Homo sapiens (human) HUVEC cells Inhibition of NADPH oxidase in Homo sapiens (human) HUVEC cells	10.1007/s00044-012-0353-y
KB	ED₅₀	>10 μg/mL Compound: 48	Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human KB cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]
MT4	CC₅₀	135.1 μg/mL Compound: 2, procyanidin B2	Cytotoxicity against human MT4 cells by MTT assay Cytotoxicity against human MT4 cells by MTT assay	[PMID: 10425115]
MT4	IC₅₀	17.2 μg/mL Compound: 2, procyanidin B2	Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay Antiviral activity against HIV infected in human MT4 cells assessed as inhibition of virus-induced cytopathic effect by MTT assay	[PMID: 10425115]
TE-671	ED₅₀	>10 μg/mL Compound: 48	Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay Cytotoxicity against human TE671 cells by tetrazolium salt-based colorimetric assay	[PMID: 1431932]

In Vitro

Procyanidin B2 shows antiproliferative activity to MCF-7 cells, with an IC₅₀ of 19.21 μM. However, Procyanidin B2 exhibits no effect on DNA-ladder formation^[1]. Procyanidin B2 (0.1, 1, 2 μM) inhibits the activation of pyrin domain containing 3 (NLRP3) inflammasome in human umbilical vein ECs (HUVECs), and the inhibition is via suppression of AP-1 activity, and such effect can be abolished by overexpression of c-Jun. Procyanidin B2 (2 μM for 12 h) also reduces ROS in HUVECs^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Procyanidin B2 (40, 20, and 10 mg/kg, p.o.) protects against cerebral ischemia-induced infarct volume and brain edema in rats. Procyanidin B2 (40 mg/kg, p.o) also improves functional outcomes, regulates blood-brain barrier (BBB) permeability after cerebral ischemia. Moreover, Procyanidin B2 attenuates cerebral ischemia-induced tight junction degradation, mitochondrial depolarization and intracellular oxidative stress. Procyanidin B2 (40 mg/kg, p.o) increases Nrf2 activation and HO-1, GSTα, and NQO1 protein expression in normal brains in vivo^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 29106-49-8
Appearance Solid
Molecular Weight 578.52
Formel C₃₀H₂₆O₁₂
Color Light yellow to light brown
SMILES

O[C@H]1[C@@H](C2=CC=C(O)C(O)=C2)OC3=CC(O)=CC(O)=C3[C@@H]1C4=C5C(C[C@@H](O)[C@@H](C6=CC=C(O)C(O)=C6)O5)=C(O)C=C4O
Synonyms

Proanthocyanidin B2
Structure Classification
- Flavonoids
- Biflavones
- Phenols
- Polyphenols
Initial Source
- Plants
- other families
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (17)

All Histological Imaging/StainingFlow CytometryWBIHCCell Imaging/Staining

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Journal Impact Factor

Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 May 31:489:144992. PMID: 40466530
Food Chem

Discovery of novel ascorbic acid derivatives and other metabolites in fruit of Rosa roxburghii Tratt through untargeted metabolomics and feature-based molecular networking. [Abstract]

2023 Mar 30;405(Pt A):134807. PMID: 36370576
Cell Death Dis

Mitochondrial PKM2 deacetylation by procyanidin B2-induced SIRT3 upregulation alleviates lung ischemia/reperfusion injury. [Abstract]

2022 Jul 11;13(7):594. PMID: 35821123

Procyanidin B2 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 11;13(7):594. [Abstract]

The indicated dosages of PCB2 (Procyanidin B2) were injected intraperitoneally for three consecutive days before the operation. Representative images of the H&E-stained lung tissue.

Procyanidin B2 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 11;13(7):594. [Abstract]

A549 cells were treated with 20 μM PCB2 (Procyanidin B2) for 24 hours, followed by 6 hours of hypoxia and then 2 hours of reoxygenation to establish a hypoxia/reoxygenation injury model. A representative FACS scatter plot of Annexin V and propidium iodide dual labeling is shown.

Procyanidin B2 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 11;13(7):594. [Abstract]

A549 cells were treated with 20 μM PCB2 (Procyanidin B2) for 24 hours, followed by 6 hours of hypoxia and then 2 hours of reoxygenation to establish a hypoxia/reoxygenation injury model. The expression of Bcl-2 protein in lung tissue was investigated.

Procyanidin B2 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 11;13(7):594. [Abstract]

C57BL/6 mice were intraperitoneally injected with 30 mg/kg PCB2 (Procyanidin B2) for three consecutive days. SIRT3 immunohistochemical staining was performed.

Procyanidin B2 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jul 11;13(7):594. [Abstract]

I/R mice were subjected to 1 hour of ischemia and 2 hours of reperfusion to induce lung I/R injury. Control mice underwent sham surgery. Preoperative intraperitoneal injections of PCB2 (Procyanidin B2: 30 mg/kg) were administered for three consecutive days prior to surgery. Representative images of A549 cells stained with TUNEL; cell nuclei were stained with DAPI.
Phytomedicine

Procyanidins inhibit alphacoronavirus infection by reducing interferon antagonism. [Abstract]

2025 May:140:156549. PMID: 40023969
Food Res Int

Slow starch digestion in the rice cooked with adzuki bean: Contribution of procyanidins and the oxidation products. [Abstract]

2019 May;119:187-195. PMID: 30884648
Antioxidants (Basel)

Procyanidin B2 Reduces Vascular Calcification through Inactivation of ERK1/2-RUNX2 Pathway. [Abstract]

2021 Jun 5;10(6):916. PMID: 34198832
J Ethnopharmacol

Procyanidin B2 attenuates microvascular dysfunction in diabetic retinopathy via inhibition of caspase-1/GSDMD mediated pyroptosis. [Abstract]

2025 Mar 26:344:119528. PMID: 39986358
Food Funct

Grape seed procyanidins suppress the apoptosis and senescence of chondrocytes and ameliorates osteoarthritis via the DPP4-Sirt1 pathway. [Abstract]

2020 Dec 1;11(12):10493-10505. PMID: 33175932
Int J Mol Sci

Procyanidin B2 Alleviates Heat-Induced Oxidative Stress through the Nrf2 Pathway in Bovine Mammary Epithelial Cells. [Abstract]

2022 Jul 14;23(14):7769. PMID: 35887117
Int Immunopharmacol

Procyanidin B2 ameliorates the progression of osteoarthritis: An in vitro and in vivo study. [Abstract]

2022 Dec;113(Pt A):109336. PMID: 36274486
BMC Complement Med Ther

Procyanidin B2 induces apoptosis and autophagy in gastric cancer cells by inhibiting Akt/mTOR signaling pathway. [Abstract]

2021 Feb 24;21(1):76. PMID: 33627124
Andrology

2020 Dec 5.
Int J Food Sci Nutr

Contribution of amylose-procyanidin complexes to slower starch digestion of red-colored rice prepared by cooking with adzuki bean. [Abstract]

2020 Sep;71(6):715-725. PMID: 31986936
Mol Immunol

Preconditioning with procyanidin B2 protects MAC-T cells against heat exposure-induced mitochondrial dysfunction and inflammation. [Abstract]

2022 May 7;147:126-135. PMID: 35537356
Theriogenology

Procyanidin B2 improves the in vitro maturation of porcine oocytes and subsequent embryonic development. [Abstract]

2025 Jun 25:246:117555. PMID: 40580853
Exp Ther Med

Procyanidin B2 suppresses hyperglycemia-induced renal mesangial cell dysfunction by modulating CAV-1-dependent signaling. [Abstract]

2022 Jun 7;24(2):496. PMID: 35837062
Massey University

2023 Aug.

Lösungsmittel & Löslichkeit

In Vitro:

H₂O : 66.67 mg/mL (115.24 mM; Need ultrasonic)

DMSO : ≥ 50 mg/mL (86.43 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.7285 mL

8.6427 mL

17.2855 mL

5 mM

0.3457 mL

1.7285 mL

3.4571 mL

10 mM

0.1729 mL

0.8643 mL

1.7285 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS

Solubility: 50 mg/mL (86.43 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact

MedChemExpress (MCE) .

Protokoll

Cell Assay^[1]

MCF-7 cells are grown in 5 mL of RPMI 1640 medium supplemented with 20% fetal bovine serum, penicillin (100 U/mL) and streptomycin (100 μg/mL). Cells are maintained at 37°C in a humidified atmosphere of 5% CO₂ in air and subcultured every 3 days. Exponentially growing cells are plated at a seeding density of 2×10⁴ cells/mL, in 96-well plates or in dishes. After overnight incubation to allow for attachment, the cells are exposed for 24 or 48 h to various concentrations of Procyanidin B2 (0.5; 1.0; 5.0; 10.0; 25.0 and 50.0 μM) diluted in distilled water. Two millimolar cyclophosphamide (CP) is used as the positive control and solutions are sterilised by filtration^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[3]

To observe the effect of Procyanidin B2 on infarct size or brain edema, 40 rats are randomLy separated into five groups, and each group is administered with vehicle (equivalent dose of 0.9% saline administered via gavage) or 40, 20, or 10 mg/kg of Procyanidin B2, respectively. To elucidate the effect of Procyanidin B2 on blood-brain barrier (BBB) permeability, the rats (n = 6 per group for Evans blue extravasation and n = 4 per group for IgG leakage) are randomLy separated into two groups: vehicle and Procyanidin B2 (40 mg/kg). Procyanidin B2 (40 mg/kg) is administered intragastrically once a day starting at 3 h after middle cerebral artery occlusion (MCAO). For other observations, including immunohistological staining and western blot analysis, rats are randomLy separated into three groups: sham-operated, vehicle, and Procyanidin B2 (n = 4 per group). Procyanidin B2 (40 mg/kg) is administered intragastrically once a day starting at 3 h after MCAO. To elucidate the improvement in neurological function after ischemic stroke in rats, the rats (n = 8 per group) undergo neurobehavioral assays to evaluate the functional outcome after administration of Procyanidin B2 (40 mg/kg) once a day starting at 24 h after MCAO. The neurological deficits are assessed at 1, 3, 7, 11, and 14 days after MCAO^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (287 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Avelar MM, et al. Procyanidin b2 cytotoxicity to mcf-7 human breast adenocarcinoma cells. Indian J Pharm Sci. 2012 Jul;74(4):351-5. [Content Brief]

[2]. Yang H, et al. Procyanidin B2 inhibits NLRP3 inflammasome activation in human vascular endothelial cells. Biochem Pharmacol. 2014 Dec 15;92(4):599-606. [Content Brief]

[3]. Wu S, et al. Procyanidin B2 attenuates neurological deficits and blood-brain barrier disruption in a rat model of cerebral ischemia. Mol Nutr Food Res. 2015 Oct;59(10):1930-41. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / H₂O	1 mM	1.7285 mL	8.6427 mL	17.2855 mL	43.2137 mL
	5 mM	0.3457 mL	1.7285 mL	3.4571 mL	8.6427 mL
	10 mM	0.1729 mL	0.8643 mL	1.7285 mL	4.3214 mL
	15 mM	0.1152 mL	0.5762 mL	1.1524 mL	2.8809 mL
	20 mM	0.0864 mL	0.4321 mL	0.8643 mL	2.1607 mL
	25 mM	0.0691 mL	0.3457 mL	0.6914 mL	1.7285 mL
	30 mM	0.0576 mL	0.2881 mL	0.5762 mL	1.4405 mL
	40 mM	0.0432 mL	0.2161 mL	0.4321 mL	1.0803 mL
	50 mM	0.0346 mL	0.1729 mL	0.3457 mL	0.8643 mL
	60 mM	0.0288 mL	0.1440 mL	0.2881 mL	0.7202 mL
	80 mM	0.0216 mL	0.1080 mL	0.2161 mL	0.5402 mL
H₂O	100 mM	0.0173 mL	0.0864 mL	0.1729 mL	0.4321 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Keywords

Procyanidin B2

17 Cited Publications

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Procyanidin B2

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Publications Citing Use of MedChemExpress (MCE) Procyanidin B2

Biologische Aktivität

Chemical Information

Publications (17)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Procyanidin B2 Related Classifications

Art. -Nr.: HY-N0796