Art. -Nr.: HY-149006

CK156

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CK156, a chemical probe, is a highly selective death-associated protein kinase (DAPK) inhibitor with IC₅₀s of 182 nM,34 μM, and 39 μM in the DRAK1 NanoBRET assay for DRAK1, CK2a1, and CK2a2, respectively. CK156 can be used for the research of autoimmune and inflammatory diseases.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 99.60%
CAS. Nr.: 2910938-59-7
Formel: C₂₁H₂₅N₅O₃
Molecular Weight:395.45

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

CK156 Chemical Structure

CAS. Nr.: 2910938-59-7

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Cellular Effect

Cell Line	Type	Value	Description	References
D-247MG	EC₅₀	2.75 μM Compound: 34; CK156	Antiproliferative activity against human D-247MG cells assessed as reduction in cell viability after 1 to 7 days by resazurin dye based fluorimetric analysis Antiproliferative activity against human D-247MG cells assessed as reduction in cell viability after 1 to 7 days by resazurin dye based fluorimetric analysis	[PMID: 35608370]
HEK-293T	EC₅₀	>10 μM Compound: 34; CK156	Cytotoxicity against human HEK293T cells assessed as increase in total apoptotic cells incubated for 36 hrs by Annexin V staining based method Cytotoxicity against human HEK293T cells assessed as increase in total apoptotic cells incubated for 36 hrs by Annexin V staining based method	[PMID: 35608370]
LN-18	EC₅₀	11.23 μM Compound: 34; CK156	Antiproliferative activity against human LN-18 cells assessed as reduction in cell viability after 1 to 7 days by resazurin dye based fluorimetric analysis Antiproliferative activity against human LN-18 cells assessed as reduction in cell viability after 1 to 7 days by resazurin dye based fluorimetric analysis	[PMID: 35608370]
LN-229	EC₅₀	9.2 μM Compound: 34; CK156	Antiproliferative activity against human LN-229 cells assessed as reduction in cell viability after 1 to 7 days by resazurin dye based fluorimetric analysis Antiproliferative activity against human LN-229 cells assessed as reduction in cell viability after 1 to 7 days by resazurin dye based fluorimetric analysis	[PMID: 35608370]
T98G	EC₅₀	10.41 μM Compound: 34; CK156	Antiproliferative activity against human T98G cells assessed as reduction in cell viability measured after 1 to 7 days by resazurin dye based fluorimetric analysis Antiproliferative activity against human T98G cells assessed as reduction in cell viability measured after 1 to 7 days by resazurin dye based fluorimetric analysis	[PMID: 35608370]

Chemical Information

CAS. Nr. 2910938-59-7
Appearance Solid
Molecular Weight 395.45
Formel C₂₁H₂₅N₅O₃
Color White to light yellow
SMILES

O=C(C1=CC=C2C=C1OCCOCCNC3=NC4=C2C=NN4C=C3)NC(C)(C)C
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (252.88 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5288 mL

12.6438 mL

25.2876 mL

5 mM

0.5058 mL

2.5288 mL

5.0575 mL

10 mM

0.2529 mL

1.2644 mL

2.5288 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.5 mg/mL (6.32 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (6.32 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: 2.5 mg/mL (6.32 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 2.5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Christian G Kurz, et al. Illuminating the Dark: Highly Selective Inhibition of Serine/Threonine Kinase 17A with Pyrazolo[1,5- a]pyrimidine-Based Macrocycles. J Med Chem. 2022 Jun 9;65(11):7799-7817. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5288 mL	12.6438 mL	25.2876 mL	63.2191 mL
	5 mM	0.5058 mL	2.5288 mL	5.0575 mL	12.6438 mL
	10 mM	0.2529 mL	1.2644 mL	2.5288 mL	6.3219 mL
	15 mM	0.1686 mL	0.8429 mL	1.6858 mL	4.2146 mL
	20 mM	0.1264 mL	0.6322 mL	1.2644 mL	3.1610 mL
	25 mM	0.1012 mL	0.5058 mL	1.0115 mL	2.5288 mL
	30 mM	0.0843 mL	0.4215 mL	0.8429 mL	2.1073 mL
	40 mM	0.0632 mL	0.3161 mL	0.6322 mL	1.5805 mL
	50 mM	0.0506 mL	0.2529 mL	0.5058 mL	1.2644 mL
	60 mM	0.0421 mL	0.2107 mL	0.4215 mL	1.0537 mL
	80 mM	0.0316 mL	0.1580 mL	0.3161 mL	0.7902 mL
	100 mM	0.0253 mL	0.1264 mL	0.2529 mL	0.6322 mL

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CK156

MCE Bioactive Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

CK156 Related Classifications

HY-149006 Related Classifications