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Art. -Nr.: HY-174862

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dASK1

dASK1 is a selective and cereblon (CRBN)-based PROTAC degrader of apoptosis signal-regulating kinase 1 (ASK1). dASK1 forms a stable ternary complex with ASK1, facilitating ASK1 rapid and sustained degradation via the ubiquitin-proteasome pathway. dASK1 demonstrates potent ASK1 degradation. dASK1 can be used for the research of steatohepatitis.
(Pink: ASK1 ligand (HY-174860); Blue: Cereblon ligand (HY-10984); Black: linker).

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

CAS. Nr.: 3064086-31-0
Formel: C₃₈H₃₈F₂N₁₀O₈
Molecular Weight:800.77

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (269 KB)

dASK1 Chemical Structure

CAS. Nr.: 3064086-31-0

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

dASK1 is a selective and cereblon (CRBN)-based PROTAC degrader of apoptosis signal-regulating kinase 1 (ASK1). dASK1 forms a stable ternary complex with ASK1, facilitating ASK1 rapid and sustained degradation via the ubiquitin-proteasome pathway. dASK1 demonstrates potent ASK1 degradation. dASK1 can be used for the research of steatohepatitis^[1]. (Pink: ASK1 ligand (HY-174860); Blue: Cereblon ligand (HY-10984); Black: linker).

IC₅₀ & Target^[1]

Cereblon

Alle PROTACs Isoform-spezifische Produkte anzeigen :

In Vitro

dASK1 (Compound 35) (1-5000 nM, 4-12 h) induces degradation of ASK1 in HepG2 and HEK293A cells^[1].

dASK1 (1 nM to 10 μM, 24 h) shows no detectable toxicity in HepG2 cells^[1].

dASK1 (10-200 nM, 8 h) shows proteasome dependence in degrading ASK1 in HepG2 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

HepG2 cells
Concentration:

1, 10, 50, 100, 500, and 1000 nM
Incubation Time:

4, 8, and 12 h
Result:

Demonstrated the highest degradation efficacy, achieving a maximum reduction of 70% in ASK1 protein levels at 100 nM.
Showed the most significant reduction in ASK1 protein levels at 8 h.

Western Blot Analysis^[1]

Cell Line:

HEK293A cells
Concentration:

1, 10, 50, 100, 500, 1000, and 5000 nM
Incubation Time:

8 h
Result:

Demonstrated the highest degradation efficacy at 10 nM.

Cell Cytotoxicity Assay^[1]

Cell Line:

HepG2 cells
Concentration:

1, 10, 50, 100, 500, 1000, and 10000 nM
Incubation Time:

24 h
Result:

Showed no detectable toxicity.

Western Blot Analysis^[1]

Cell Line:

HepG2 cells
Concentration:

10, 50, 100, and 200 nM; 10 and 50 nM (pre-treating with 5 µM of MG132 (HY-13259) for 30 min)
Incubation Time:

8 h
Result:

Degraded ASK1 levels.
MG132 (HY-13259) blocked degradation and led to ASK1 accumulation.

Chemical Information

CAS. Nr. 3064086-31-0
Molecular Weight 800.77
Formel C₃₈H₃₈F₂N₁₀O₈
SMILES

O=C(NCCOCCNC1=CC=CC(C(N2C3C(NC(CC3)=O)=O)=O)=C1C2=O)CCC(NC4=C(F)C=C(F)C(C(NC5=NC(C6=NN=CN6C(C)C)=CC=C5)=O)=C4)=O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (269 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Sarkar HS, et al. Rational design and discovery of potent PROTAC degraders of ASK1: a targeted therapy in MASH. RSC Med Chem. 2025 Jun 6. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

MOQ

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100 mg

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Reinheit & Dokumentation
Verweise

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dASK1

Alle PROTACs Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Reinheit & Dokumentation

Verweise

Calculators

Help & FAQs

dASK1 Related Classifications

HY-174862 Related Classifications