Art. -Nr.: HY-N7591

Millepachine

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Millepachine is a bioactive natural chalcone from Chinese herbal medicine Millettia pachycarpa Benth, exhibits strong antitumor effects against numerous human cancer cells both in vitro and in vivo.

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Reinheit: 98.81%
CAS. Nr.: 1393922-01-4
Formel: C₂₂H₂₂O₄
Molecular Weight:350.41

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Millepachine Chemical Structure

CAS. Nr.: 1393922-01-4

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	3.09 μM Compound: Millepachine	Antiproliferative activity against human A2780 cells assessed as cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human A2780 cells assessed as cell viability incubated for 48 hrs by MTT assay	[PMID: 35282680]
A-375	IC₅₀	3.58 μM Compound: Millepachine	Cytotoxicity against human A375 cells by MTT assay Cytotoxicity against human A375 cells by MTT assay	[PMID: 23425970]
A-375	IC₅₀	3580 nM Compound: 1	Antiproliferative activity against human A375 cells assessed as inhibition of cell proliferation after 48 hrs by MTT assay Antiproliferative activity against human A375 cells assessed as inhibition of cell proliferation after 48 hrs by MTT assay	[PMID: 25208345]
A549	IC₅₀	>10 μM Compound: Millepachine	Cytotoxicity against human A549 cells incubated for 72 hrs by MTT assay Cytotoxicity against human A549 cells incubated for 72 hrs by MTT assay	[PMID: 38452727]
A549	IC₅₀	3.95 μM Compound: Millepachine	Antiproliferative activity against human A549 cells assessed as cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as cell viability incubated for 48 hrs by MTT assay	[PMID: 35282680]
HCT-116	IC₅₀	4.66 μM Compound: Millepachine	Antiproliferative activity against human HCT116 cells after 24 hrs by MTT assay Antiproliferative activity against human HCT116 cells after 24 hrs by MTT assay	[PMID: 22784822]
HCT-8	IC₅₀	5.02 μM Compound: Millepachine	Antiproliferative activity against human HCT-8 cells assessed as cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human HCT-8 cells assessed as cell viability incubated for 48 hrs by MTT assay	[PMID: 35282680]
HeLa	IC₅₀	6.03 μM Compound: Millepachine	Antiproliferative activity against human HeLa cells assessed as cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human HeLa cells assessed as cell viability incubated for 48 hrs by MTT assay	[PMID: 35282680]
HepG2	IC₅₀	1.51 μM Compound: Millepachine	Antiproliferative activity against human HepG2 cells after 24 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 22784822]
HepG2	IC₅₀	1.51 μM Compound: Millepachine	Cytotoxicity against human HepG2 cells by MTT assay Cytotoxicity against human HepG2 cells by MTT assay	[PMID: 23425970]
HepG2	IC₅₀	1.51 μM Compound: Millepachine	Antiproliferative activity against human HepG2 cells after 24 hrs by MTT assay Antiproliferative activity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 24629450]
HepG2	IC₅₀	1.51 μM Compound: 5	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability Cytotoxicity against human HepG2 cells assessed as reduction in cell viability	[PMID: 31539776]
HepG2	IC₅₀	1510 nM Compound: 1	Antiproliferative activity against human HepG2 cells assessed as inhibition of cell proliferation after 48 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as inhibition of cell proliferation after 48 hrs by MTT assay	[PMID: 25208345]
HepG2	IC₅₀	4.54 μM Compound: 1a	Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay Cytotoxicity against human HepG2 cells after 48 hrs by MTT assay	[PMID: 28340411]
HT-29	IC₅₀	3.29 μM Compound: Millepachine	Antiproliferative activity against human HT-29 cells after 24 hrs by MTT assay Antiproliferative activity against human HT-29 cells after 24 hrs by MTT assay	[PMID: 22784822]
K562	IC₅₀	4.56 μM Compound: Millepachine	Antiproliferative activity against human K562 cells after 24 hrs by MTT assay Antiproliferative activity against human K562 cells after 24 hrs by MTT assay	[PMID: 22784822]
K562	IC₅₀	4.56 μM Compound: Millepachine	Cytotoxicity against human K562 cells by MTT assay Cytotoxicity against human K562 cells by MTT assay	[PMID: 23425970]
K562	IC₅₀	4560 nM Compound: 1	Antiproliferative activity against human K562 cells assessed as inhibition of cell proliferation after 48 hrs by MTT assay Antiproliferative activity against human K562 cells assessed as inhibition of cell proliferation after 48 hrs by MTT assay	[PMID: 25208345]
L02	IC₅₀	>20 μM Compound: Millepachine	Cytotoxicity against human LO2 cells by MTT assay Cytotoxicity against human LO2 cells by MTT assay	[PMID: 23425970]
L02	IC₅₀	25.05 μM Compound: 1a	Cytotoxicity against human HL7702 cells after 48 hrs by MTT assay Cytotoxicity against human HL7702 cells after 48 hrs by MTT assay	[PMID: 28340411]
MCF7	IC₅₀	4.37 μM Compound: Millepachine	Cytotoxicity against human MCF7 cells incubated for 72 hrs by MTT assay Cytotoxicity against human MCF7 cells incubated for 72 hrs by MTT assay	[PMID: 38452727]
MCF7	IC₅₀	6.98 μM Compound: Millepachine	Antiproliferative activity against human MCF7 cells assessed as cell viability incubated for 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as cell viability incubated for 48 hrs by MTT assay	[PMID: 35282680]
MDA-MB-231	IC₅₀	2.47 μM Compound: Millepachine	Cytotoxicity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells incubated for 72 hrs by MTT assay	[PMID: 38452727]
MGC-803	IC₅₀	10.98 μM Compound: 1a	Cytotoxicity against human MGC803 cells after 48 hrs by MTT assay Cytotoxicity against human MGC803 cells after 48 hrs by MTT assay	[PMID: 28340411]
NCI-H460	IC₅₀	7.65 μM Compound: 1a	Cytotoxicity against human NCI-H460 cells after 48 hrs by MTT assay Cytotoxicity against human NCI-H460 cells after 48 hrs by MTT assay	[PMID: 28340411]
PC-3	IC₅₀	1.13 μM Compound: Millepachine	Cytotoxicity against human PC-3 cells incubated for 72 hrs by MTT assay Cytotoxicity against human PC-3 cells incubated for 72 hrs by MTT assay	[PMID: 38452727]
SK-OV-3	IC₅₀	2.36 μM Compound: Millepachine	Antiproliferative activity against human SKOV3 cells after 24 hrs by MTT assay Antiproliferative activity against human SKOV3 cells after 24 hrs by MTT assay	[PMID: 22784822]
SK-OV-3	IC₅₀	9.85 μM Compound: 1a	Cytotoxicity against human SKOV3 cells after 48 hrs by MTT assay Cytotoxicity against human SKOV3 cells after 48 hrs by MTT assay	[PMID: 28340411]
SW-620	IC₅₀	0.76 μM Compound: Millepachine	Antiproliferative activity against human SW620 cells after 24 hrs by MTT assay Antiproliferative activity against human SW620 cells after 24 hrs by MTT assay	[PMID: 22784822]
T-24	IC₅₀	11.05 μM Compound: 1a	Cytotoxicity against human T24 cells after 48 hrs by MTT assay Cytotoxicity against human T24 cells after 48 hrs by MTT assay	[PMID: 28340411]
U-251	IC₅₀	3.72 μM Compound: Millepachine	Cytotoxicity against human U-251 cells incubated for 72 hrs by MTT assay Cytotoxicity against human U-251 cells incubated for 72 hrs by MTT assay	[PMID: 38452727]

In Vitro

Millepachine (1.25-20 μM; 48 h) remarkably inhibits the proliferation of cisplatin-resistant A2780CP cells^[1].
Millepachine (2-8 μM; 24 or 48 h) induces G2/M arrest and apoptosis cisplatin-sensitive A2780S and cisplatin-resistant A2780CP cells^[1].
Millepachine (2-8 μM; 24 h) decreases topoisomerase II levels in A2780S and A2780CP cells^[1].
Millepachine (2-8 μM; 24 h) inhibits ATP-binding cassette transporter activity in A2780CP cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

A2780CP cells
Concentration:

0, 1.25, 2.5, 5, 10, 20 μM
Incubation Time:

48 hours
Result:

Inhibited the cells proliferation with an IC₅₀ of 4 μM.

Cell Cycle Analysis^[1]

Cell Line:

A2780S and A2780CP cells
Concentration:

2, 4, 8 μM
Incubation Time:

24 or 48 hours
Result:

Induced significant G2/M arrest both in both cells.
The percentage of cells in the G2/M fraction increased from 15.99% in vehicle cells to 24.93%, 60.67%, and 77.31% at dose of 2, 4, and 8 μM in A2780S cells, respectively.

Apoptosis Analysis^[1]

Cell Line:

A2780S and A2780CP cells
Concentration:

2, 4, 8 μM
Incubation Time:

24 or 48 hours
Result:

The percentage of apoptotic cells increased from 1.49% in vehicle cells to 10.98%, 20.60%, and 39.43% at dose of 2, 4, and 8 μM in A2780S cells, respectively.
The percentage of apoptotic cells increased from 0.87% to 10.97%, 25.28%, and 37.59% in A2780CP cells, respectively.

Western Blot Analysis^[1]

Cell Line:

A2780S and A2780CP cells
Concentration:

0, 2, 4, 8 μM
Incubation Time:

24 hours
Result:

Decreased the levels of topoisomerase II (TOPO II) in both cells.

In Vivo

Millepachine (20 mg/kg; i.v. every two days for 14 day) inhibits tumor growth in mice^[1].
Millepachine (20 mg/kg; i.v. every two days for 14 day) does not induce acquired drug resistance in an excised A2780S xenograft model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Female BALB/c nude mice (6 weeks) are injected A2780S or A2780CP cells^[1]
Dosage:

20 mg/kg
Administration:

I.v. every two days for 2-14 days
Result:

Reduced tumor volume and tumor weight with the inhibitory rate of 73.21% and 65.58% in A2780S (after seven times injection) and A2780CP (after six times injection) xenograft model, respectively.
With low toxicity in vivo.

Chemical Information

CAS. Nr. 1393922-01-4
Appearance Solid
Molecular Weight 350.41
Formel C₂₂H₂₂O₄
Color Light yellow to yellow
SMILES

O=C(C1=C2C(C=CC(C)(C)O2)=C(OC)C=C1)/C=C/C3=CC=C(OC)C=C3
Structure Classification
- Flavonoids
- Chalcones
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (285.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8538 mL

14.2690 mL

28.5380 mL

5 mM

0.5708 mL

2.8538 mL

5.7076 mL

10 mM

0.2854 mL

1.4269 mL

2.8538 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2 mg/mL (5.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2 mg/mL (5.71 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2 mg/mL (5.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Wu W, et, al. Millepachine showed novel antitumor effects in cisplatin-resistant human ovarian cancer through inhibiting drug efflux function of ATP-binding cassette transporters. Phytother Res. 2018 Dec; 32(12):2428-2435. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.8538 mL	14.2690 mL	28.5380 mL	71.3450 mL
	5 mM	0.5708 mL	2.8538 mL	5.7076 mL	14.2690 mL
	10 mM	0.2854 mL	1.4269 mL	2.8538 mL	7.1345 mL
	15 mM	0.1903 mL	0.9513 mL	1.9025 mL	4.7563 mL
	20 mM	0.1427 mL	0.7134 mL	1.4269 mL	3.5672 mL
	25 mM	0.1142 mL	0.5708 mL	1.1415 mL	2.8538 mL
	30 mM	0.0951 mL	0.4756 mL	0.9513 mL	2.3782 mL
	40 mM	0.0713 mL	0.3567 mL	0.7134 mL	1.7836 mL
	50 mM	0.0571 mL	0.2854 mL	0.5708 mL	1.4269 mL
	60 mM	0.0476 mL	0.2378 mL	0.4756 mL	1.1891 mL
	80 mM	0.0357 mL	0.1784 mL	0.3567 mL	0.8918 mL
	100 mM	0.0285 mL	0.1427 mL	0.2854 mL	0.7134 mL

Keywords

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Millepachine

Apoptosis Compound Library

Natural Product Library

Anti-Cancer Compound Library

Flavonoids Library

Anti-Cancer Natural Product Library

Plant-Sourced Natural Product Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

RO5 Drug-like Natural Product Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Natural Product Diversity Scaffold Library

Mass Spectrometry Natural Product Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Millepachine

Apoptosis Compound Library

Natural Product Library

Anti-Cancer Compound Library

Flavonoids Library

Anti-Cancer Natural Product Library

Plant-Sourced Natural Product Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

RO5 Drug-like Natural Product Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Natural Product Diversity Scaffold Library

Mass Spectrometry Natural Product Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Millepachine Related Classifications

HY-N7591 Related Classifications