Art. -Nr.: HY-183716

Mito-TP-2

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Mito-TP-2 is a triptolide (HY-32735) derivative. Mito-TP-2 exhibits concentration-dependent cytotoxicity in cancer cells. Mito-TP-2 is selectively driven and accumulated into the mitochondria of tumor cells by mitochondrial transmembrane potential and exerts specific mitochondrial toxicity. Mito-TP-2 can be used for the research of liver cancer, breast cancer, and non-small cell lung cancer.

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Reinheit: 96.82%
CAS. Nr.: 2489184-70-3
Formel: C₄₄H₄₈BrO₇P
Molecular Weight:799.73

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Mito-TP-2 Chemical Structure

CAS. Nr.: 2489184-70-3

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	10 μM Compound: Mito-TP-2	Cytotoxicity against human A549 cells assessed as inhibition of cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as inhibition of cell viability incubated for 48 hrs by MTT assay	[PMID: 38169372]

In Vitro

Mito-TP-2 (48 h) potently inhibits HepG-2 human hepatocellular carcinoma cells (IC₅₀=0.067 μM) and MCF-7 human breast cancer cells (IC₅₀=0.238 μM), while showing 16-fold reduced toxicity to L-02 human normal hepatocytes (IC₅₀=0.334 μM) compared to Triptolide (HY-32735)^[1].
Mito-TP-2 (48 h) potently inhibits NCI-H1975 cell proliferation with an IC₅₀ of 0.14 μM, while showing minimal activity against BEAS-2B, MCF-7, A549, and NCI-H460 cells^[2].
Mito-TP-2 accumulates to a higher level than Triptolide (HY-32735) in HepG2 cell mitochondria, shows enhanced antitumor selectivity, and exhibits reduced toxicity toward normal cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 2489184-70-3
Appearance Solid
Molecular Weight 799.73
Formel C₄₄H₄₈BrO₇P
Color White to off-white
SMILES

C[C@@]12[C@@]34[C@@]5([C@@](C[C@@]1([H])C6=C(C(OC6)=O)CC2)([H])O5)[C@@H]([C@@]7([C@](O7)([H])[C@]3([H])O4)C(C)C)OC(CCCCC[P+](C8=CC=CC=C8)(C9=CC=CC=C9)C%10=CC=CC=C%10)=O.[Br-]
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (125.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.2504 mL

6.2521 mL

12.5042 mL

5 mM

0.2501 mL

1.2504 mL

2.5008 mL

10 mM

0.1250 mL

0.6252 mL

1.2504 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 5 mg/mL (6.25 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 5 mg/mL (6.25 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 5 mg/mL (6.25 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Fan P, et al. Triptolide derivative and preparation method and application thereof. China, CN111518157A. 2020-08-11.

[2]. Xing W, et al. Selective Antitumor Effect and Lower Toxicity of Mitochondrion-Targeting Derivatization of Triptolide. J Med Chem. 2024;67(2):1093-1114. [Content Brief]

[3]. Zhang X, et al. Pro-drug approaches to overcome poor solubility and toxicity of triptolide. Phytochemistry Reviews. 2025 Aug 06;25:1565-1580.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.2504 mL	6.2521 mL	12.5042 mL	31.2606 mL
	5 mM	0.2501 mL	1.2504 mL	2.5008 mL	6.2521 mL
	10 mM	0.1250 mL	0.6252 mL	1.2504 mL	3.1261 mL
	15 mM	0.0834 mL	0.4168 mL	0.8336 mL	2.0840 mL
	20 mM	0.0625 mL	0.3126 mL	0.6252 mL	1.5630 mL
	25 mM	0.0500 mL	0.2501 mL	0.5002 mL	1.2504 mL
	30 mM	0.0417 mL	0.2084 mL	0.4168 mL	1.0420 mL
	40 mM	0.0313 mL	0.1563 mL	0.3126 mL	0.7815 mL
	50 mM	0.0250 mL	0.1250 mL	0.2501 mL	0.6252 mL
	60 mM	0.0208 mL	0.1042 mL	0.2084 mL	0.5210 mL
	80 mM	0.0156 mL	0.0782 mL	0.1563 mL	0.3908 mL
	100 mM	0.0125 mL	0.0625 mL	0.1250 mL	0.3126 mL

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Mito-TP-2

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Liver Cancer Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Anti-Cancer Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Mito-TP-2

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Liver Cancer Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Anti-Cancer Compound Library

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Mito-TP-2 Related Classifications

HY-183716 Related Classifications