Art. -Nr.: HY-16931

SMIFH2

10 Cited Publications

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Based on 10 publication(s) in Google Scholar

SMIFH2 is a formin specific inhibitor. SMIFH2 inhibits actin polymerization by Formins and affects the actin cytoskeleton.

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Reinheit: 99.08%
CAS. Nr.: 340316-62-3
Formel: C₁₅H₉BrN₂O₃S
Molecular Weight:377.21

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

SMIFH2 Chemical Structure

CAS. Nr.: 340316-62-3

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (10)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

SMIFH2 is a formin specific inhibitor. SMIFH2 inhibits actin polymerization by Formins and affects the actin cytoskeleton^[1].

IC₅₀ & Target

Formin^[1]

In Vitro

SMIFH2 (25 uM; 1-16 hours) induces dynamic cytoskeletal remodelling in U2OS cells^[1].
SMIFH2 (25 μM) reduces p300, mDia2 and p53 levels in a proteasome-independent manner^[1].
SMIFH2 reduces expression and activity of p53 through a post-transcriptional, proteasome-independent mechanism that influences remodelling of the cytoskeleton^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

293T, A375, U2OS, and MDA-MB-231 cells
Concentration:

25 μM
Incubation Time:

5 hours for 293T, U2OS, MDA-MB-231 cells; 2.5 hours for A375 cells
Result:

Downregulated mDia2, p53 and p300 protein levels.

Chemical Information

CAS. Nr. 340316-62-3
Appearance Solid
Molecular Weight 377.21
Formel C₁₅H₉BrN₂O₃S
Color Light yellow to green yellow
SMILES

O=C(/C(C(N1C2=CC=CC(Br)=C2)=O)=C\C3=CC=CO3)NC1=S
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (10)

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Journal Impact Factor

Nature

Submicrometre sampling of living cells by macrophages. [Abstract]

2026 Jun;654(8118):495-503. PMID: 42056510
Nat Commun

Promoting implant osseointegration via the osteoblast-selective β-amino acid polymer strategy. [Abstract]

2025 Aug 5;16(1):7190. PMID: 40764473
Adv Sci (Weinh)

ERM Inhibition Confers Ferroptosis Resistance through ROS-Induced NRF2 Signaling. [Abstract]

2026 Mar;13(16):e13310. PMID: 41589654
Int J Biol Macromol

Host factor DIAPH1 regulates pseudorabivirus replication by modulating the dynamics of cytoskeleton. [Abstract]

2025 Jan 20:140112. PMID: 39842589

SMIFH2 purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Jan 20:140112. [Abstract]

SMIFH2 (1-50 μM; 4 h) treatment resulted in a decrease in PRV-GFP proliferation, as indicated by a lower percentage of GFP-positive cells in PK-15 cells.

SMIFH2 purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Jan 20:140112. [Abstract]

SMIFH2 (5-50 μM; 4 h) caused dose-dependent inhibition of PRV replication in PK-15 cells.

SMIFH2 purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Jan 20:140112. [Abstract]

SMIFH2 (1-50 μM; 4 h) inhibited DIAPH1 and diminished the PRV viral progeny production.

SMIFH2 purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Jan 20:140112. [Abstract]

SMIFH2 (50 μM; 4 h) decreased the level of acetylated-α-tubulin in PK-15 cells.

SMIFH2 purchased from MedChemExpress. Usage Cited in: Int J Biol Macromol. 2025 Jan 20:140112. [Abstract]

SMIFH2 (50 μM; 4 h) decreased actin levels and the formation of thick, long actin stress fibers, resulting in more thin and short microfilaments in PK-15 cells.
Theriogenology

The formins inhibitor SMIFH2 inhibits the cytoskeleton dynamics and mitochondrial function during goat oocyte maturation. [Abstract]

2023 Nov:211:40-48. PMID: 37562190
Virology

The actin cytoskeleton is important for pseudorabies virus infection. [Abstract]

2024 Dec:600:110233. PMID: 39255726
Dev Comp Immunol

Development of monoclonal antibodies against IgM of common carp (Cyprinus carpio) and analysis of phagocytosis by mIgM+ B lymphocytes. [Abstract]

2026 May:178:105593. PMID: 41881180
Biomed Pharmacother

Myeloid-specific Exoc5 deficiency develops renal inflammation and hypertension. [Abstract]

2026 Feb:195:119061. PMID: 41604889
bioRxiv

2025 Aug 05.
bioRxiv

2025 April 23.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 125 mg/mL (331.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.6510 mL

13.2552 mL

26.5104 mL

5 mM

0.5302 mL

2.6510 mL

5.3021 mL

10 mM

0.2651 mL

1.3255 mL

2.6510 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.08 mg/mL (5.51 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Tadamoto Isogai, et al. SMIFH2 has effects on Formins and p53 that perturb the cell cytoskeleton. Sci Rep. 2015 Apr 30;5:9802. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.6510 mL	13.2552 mL	26.5104 mL	66.2761 mL
	5 mM	0.5302 mL	2.6510 mL	5.3021 mL	13.2552 mL
	10 mM	0.2651 mL	1.3255 mL	2.6510 mL	6.6276 mL
	15 mM	0.1767 mL	0.8837 mL	1.7674 mL	4.4184 mL
	20 mM	0.1326 mL	0.6628 mL	1.3255 mL	3.3138 mL
	25 mM	0.1060 mL	0.5302 mL	1.0604 mL	2.6510 mL
	30 mM	0.0884 mL	0.4418 mL	0.8837 mL	2.2092 mL
	40 mM	0.0663 mL	0.3314 mL	0.6628 mL	1.6569 mL
	50 mM	0.0530 mL	0.2651 mL	0.5302 mL	1.3255 mL
	60 mM	0.0442 mL	0.2209 mL	0.4418 mL	1.1046 mL
	80 mM	0.0331 mL	0.1657 mL	0.3314 mL	0.8285 mL
	100 mM	0.0265 mL	0.1326 mL	0.2651 mL	0.6628 mL

Keywords

SMIFH2

10 Cited Publications

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SMIFH2

Anti-Cancer Compound Library

Covalent Screening Library

Cytoskeleton Compound Library

Protein-protein Interaction Inhibitor Library

Heterocyclic Compound Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

Covalent Screening Library Plus

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) SMIFH2

Biologische Aktivität

Chemical Information

Publications (10)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

SMIFH2

Anti-Cancer Compound Library

Covalent Screening Library

Cytoskeleton Compound Library

Protein-protein Interaction Inhibitor Library

Heterocyclic Compound Library

Highly Selective Inhibitors Library

Bioactive Compound Library Max

Covalent Screening Library Plus

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) SMIFH2

Biologische Aktivität

Chemical Information

Publications (10)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

SMIFH2 Related Classifications

HY-16931 Related Classifications