Cuprizone
Based on 4 publication(s) in Google Scholar
Cuprizone is a copper chelating agent that forms a deep blue copper ketone complex with copper (II). The copper ketone reaction can be used in colorimetric tests for the presence of trace copper. Cuprizone can be used to induce some schizophrenia-like behavior in mice. Cuprizone acts on copper enzymes, including SOD1, cytochrome oxidase, and DβH, thereby causing oxidative stress and increasing DA levels in certain brain regions such as the medial prefrontal cortex (PFC).
For research use only. We do not sell to patients.
- Purity: 99.61%
- CAS No.: 370-81-0
- Formula: C14H22N4O2
- Molecular Weight:278.36
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Publications Citing Use of MedChemExpress (MCE) Cuprizone
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Customer Validation & Images
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In Vivo Efficacy Study
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Histological Imaging/Staining
Biological Activity
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 370-81-0
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Appearance Solid
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Molecular Weight 278.36
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Formula C14H22N4O2
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Color White to off-white
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SMILES
O=C(C(N/N=C1CCCCC\1)=O)N/N=C2CCCCC/2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (4)
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Journal Impact Factor
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Most Recent
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J Nanobiotechnology
Eriodictyol-cisplatin coated nanomedicine synergistically promote osteosarcoma cells ferroptosis and chemosensitivity. [Abstract]2025 Feb 14;23(1):109. PMID: 39953537 -
Pharmacol Res
Plumbagin ameliorates multiple sclerosis by inducing DDX3X-mediated stress granule assembly in mice. [Abstract]2026 Jul:229:108257. PMID: 42167385 -
Acta Pharmacol Sin
Activation of TRPV1 receptor facilitates myelin repair following demyelination via the regulation of microglial function. [Abstract]2023 Apr;44(4):766-779. PMID: 36229601
Cuprizone purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2023 Apr;44(4):766-779. [Abstract]
Cuprizone (CPZ) (0.3%; p.o.; 5 weeks) increased the expression of TRPV1 protein in the corpus callosum of C57BL/6 mice.
Cuprizone purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2023 Apr;44(4):766-779. [Abstract]
Cuprizone (CPZ) (0.3%; p.o.; 5 weeks) caused significant body weight loss in C57BL/6 and Trpv1-/- mice.
Cuprizone purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2023 Apr;44(4):766-779. [Abstract]
Cuprizone (CPZ) (0.3%; p.o.; 5 weeks) significantly decreased the protein levels of MBP in the corpus callosum of Trpv1-/- mice.
Cuprizone purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2023 Apr;44(4):766-779. [Abstract]
Representative immunofluorescent images of MBP staining in the corpus callosum of Trpv1-/- mice treated with Cuprizone (CPZ) (0.3%; p.o.; 5 weeks).
Cuprizone purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2023 Apr;44(4):766-779. [Abstract]
Cuprizone (CPZ) (0.3%; p.o.; 5 weeks) significantly decreased myelinated axons in C57BL/6 and Trpv1-/- mice.
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Mol Med
CYFIP1 coordinate with RNMT to induce osteosarcoma cuproptosis via AURKAIP1 m7G modification. [Abstract]2025 Feb 21;31(1):74. PMID: 39984834
Solvent & Solubility
DMSO : 6.67 mg/mL (23.96 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.67 mg/mL (2.41 mM); Clear solution
This protocol yields a clear solution of ≥ 0.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (761 KB)
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- Español - ES (761 KB)
- Swedish - SV (761 KB)
- Italian - IT (761 KB)
- Korean - KR (761 KB)
- Portuguese - PT (761 KB)
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Handling Instructions (2659 KB)
References
[1]. Suzuki K, et al. Status spongiosus of CNS and hepatic changes induced by cuprizone (biscyclohexanone oxalyldihydrazone). Am J Pathol. 1969 Feb;54(2):307-25. [Content Brief]
[2]. Sanadgol N, et al. Alpha-lipoic acid mitigates toxic-induced demyelination in the corpus callosum by lessening of oxidative stress and stimulation of polydendrocytes proliferation. Metab Brain Dis. 2018 Feb;33(1):27-37. [Content Brief]
[3]. M Jake Pushie, et al. Synthesis and structural characterization of copper-cuprizone complexes. Dalton Trans. 2022 Jun 29. [Content Brief]
[4]. Haiyun Xu, et al. Behavioral and neurobiological changes in C57BL/6 mouse exposed to cuprizone: effects of antipsychotics. Front Behav Neurosci. 2010 Mar 18;4:8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.5925 mL | 17.9624 mL | 35.9247 mL | 89.8118 mL |
| 5 mM | 0.7185 mL | 3.5925 mL | 7.1849 mL | 17.9624 mL | |
| 10 mM | 0.3592 mL | 1.7962 mL | 3.5925 mL | 8.9812 mL | |
| 15 mM | 0.2395 mL | 1.1975 mL | 2.3950 mL | 5.9875 mL | |
| 20 mM | 0.1796 mL | 0.8981 mL | 1.7962 mL | 4.4906 mL |
Cuprizone Related Classifications
HY-W115718 Related Classifications
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