DCG04
Based on 1 publication(s) in Google Scholar
DCG04 is a biotinylated activity-based probe. DCG04 labels calpain-1 and is applicable for affinity purification and mass spectrometry identification. DCG04 is used in cancer and malaria research.
For research use only. We do not sell to patients.
- Purity : 98.55%
- CAS No.: 314263-42-8
- Formula: C43H66N8O11S
- Molecular Weight:903.10
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) DCG04
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Biological Activity
Description
In Vitro
DCG04 reveals a progressive increase in cathepsin activity during RIP1-Tag2 tumorigenesis, identifying cathepsins B, C, L, and Z as active proteases in tumors[1].
DCG04 profiling of flow-sorted RIP1-Tag2 tumor cells shows that infiltrating innate immune cells are a significant source of cathepsin activity, with distinct activity profiles among endothelial, immune, and tumor cell fractions[1].
DCG-04 (50 μM) is an activity-dependent affinity label that covalently modifies multiple active cysteine proteases in DC2.4 dendritic cell extracts at pH 5.5[2].
DCG-04 affinity labeling enriches a 27-kDa cysteine protease from bovine chromaffin granules that is responsible for PE-processing activity[3].
DCG04 activity labeling confirms that immunodepletion and calpastatin loading abolish calpain-1 activity in resealed erythrocytes, which is restored by calpain-1 reconstitution[8].
DCG04 (4 μM; 4 h) is a component of the biotinylated probe cocktail used for ABPP-MS to display active cysteine proteases in N. benthamiana apoplastic fluid, revealing that bacterial infection reduces the activity of nine cysteine proteases[11].
DCG04 (~48 hours postinfection) labels a single ~80 kD membrane-associated protein in P. falciparum-infected erythrocytes, identified as human calpain-1[8].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 314263-42-8
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Appearance Solid
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Molecular Weight 903.10
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Formula C43H66N8O11S
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Color White to off-white
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SMILES
OC(C=C1)=CC=C1C[C@@H](C(NCCCCCC(N[C@H](C(N)=O)CCCCNC(CCCC[C@H]2[C@]3([H])[C@](NC(N3)=O)([H])CS2)=O)=O)=O)NC([C@H](CC(C)C)NC([C@@H]4[C@H](O4)C(OCC)=O)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
In Vitro:
DMSO : 3.33 mg/mL (3.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Recombinant antibody expression and Protein A/G purification
Recombinant antibody expression produces antibody chains from cloned antibody genes in mammalian cells, commonly HEK293-derived cells, so that secreted IgG or Fc-fusion antibodies can be collected from culture supernatant. Protein A/G purification is affinity chromatography in which immobilized Protein A, Protein G, or Protein A/G binds the antibody Fc region; nonbound culture components are washed away, and bound antibody is recovered by changing buffer conditions, commonly acidic elution followed by neutralization.
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Protocol For Protein Expression And Purification
Recombinant protein expression in Escherichia coli followed by purification of a His-tagged soluble protein by immobilized metal affinity chromatography (IMAC), with optional MBP fusion and TEV tag removal when the construct includes these elements. The biological readout is production of the encoded target protein, detected as an inducible band at the expected molecular mass by SDS-PAGE and quantified by total protein assay or chromatographic absorbance; the purification readout is enrichment of the target protein in elution fractions after selective binding of polyhistidine residues to immobilized Ni2+/metal-chelate resin and elution by imidazole-containing buffer. Expression is driven by an inducible bacterial expression system, commonly T7/lac-based, in which IPTG or lactose/auto-induction activates transcription and translation of the cloned gene; lower induction temperature, lower inducer concentration, induction timing, and solubility-enhancing fusion tags can influence the frac
Purity & Documentation
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Data Sheet (280 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.1073 mL | 5.5365 mL | 11.0730 mL | 27.6824 mL |
DCG04 Related Classifications
HY-101779 Related Classifications
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