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AY 9944 

Cat. No.: HY-107420 Purity: 99.88%
Handling Instructions

AY 9944 is a specific cholesterol biosynthesis inhibitor. AY 9944 inhibits the 7-dehydro cholesterol Δ7-reductase (DHCR7) enzyme with an IC50 of 13 nM. AY 9944 causes hypocholesterolemia and accumulation of 7DHC. At high doses, AY 9944 inhibits also in cultured embryos sterol Δ7-Δ8 isomerase, which causes the accumulation of cholest-8-en-3β-ol.

For research use only. We do not sell to patients.

AY 9944 Chemical Structure

AY 9944 Chemical Structure

CAS No. : 366-93-8

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Based on 1 publication(s) in Google Scholar

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Description

AY 9944 is a specific cholesterol biosynthesis inhibitor. AY 9944 inhibits the 7-dehydro cholesterol Δ7-reductase (DHCR7) enzyme with an IC50 of 13 nM. AY 9944 causes hypocholesterolemia and accumulation of 7DHC. At high doses, AY 9944 inhibits also in cultured embryos sterol Δ7-Δ8 isomerase, which causes the accumulation of cholest-8-en-3β-ol[1][2][3].

IC50 & Target

IC50: 13 nM (DHCR7)1

In Vitro

AY 9944 (1 μg/mL; 15 h) affects types of free sterol in keratinocytes[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: Human foreskin keratinocytes
Concentration: 1 μg/mL
Incubation Time: 15 hours
Result: Inhibited the synthesis of cholesterol and desmosterol, but increased 7-dehydrocholesterol and zymosterol synthesis.
In Vivo

AY 9944 (7.5 mg/kg; i.h. every 6 days once from postnatal day 2 to postnatal day 20) reduces brain cholesterol until 400 days after stop treatment[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Long–Evans hooded rats[3].
Dosage: 7.5 mg/kg
Administration: Subcutaneous injection; 7.5 mg/kg every 6 days once; from postnatal day 2 to postnatal day 20
Result: Reduced brain cholesterol and increased 7-dehydrocholesterol in all brain regions of rats till the treatment stopped for 400 days and showed a more severe effect on the change of sterols in plasma and liver of female rats.
Molecular Weight

464.30

Formula

C22H30Cl4N2

CAS No.
SMILES

ClC(C=CC=C1)=C1CNC[[email protected]](CC2)CC[[email protected]@H]2CNCC3=C(C=CC=C3)Cl.[H]Cl.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 5 mg/mL (10.77 mM; Need ultrasonic)

H2O : 5 mg/mL (10.77 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1538 mL 10.7689 mL 21.5378 mL
5 mM 0.4308 mL 2.1538 mL 4.3076 mL
10 mM 0.2154 mL 1.0769 mL 2.1538 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation

Purity: 99.88%

References
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Product Name:
AY 9944
Cat. No.:
HY-107420
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