1. TGF-beta/Smad
  2. TGF-β Receptor
  3. BMP signaling agonist sb4

BMP signaling agonist sb4 

Cat. No.: HY-124697 Purity: 99.86%
Handling Instructions

BMP signaling agonist sb4 is a potent benzoxazole bone morphogenetic protein 4 (BMP4) signaling agonist with a EC50 value of 74 nM, activates BMP signaling by stabilizing intracellular p-SMAD-1/5/9. BMP signaling agonist sb4 activates BMP4 target genes (inhibitors of DNA binding, Id1 and Id3) canonical BMP signaling.

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BMP signaling agonist sb4 Chemical Structure

BMP signaling agonist sb4 Chemical Structure

CAS No. : 100874-08-6

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10 mM * 1  mL in DMSO USD 80 In-stock
Estimated Time of Arrival: December 31
10 mg USD 80 In-stock
Estimated Time of Arrival: December 31
50 mg USD 210 In-stock
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100 mg USD 375 In-stock
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Description

BMP signaling agonist sb4 is a potent benzoxazole bone morphogenetic protein 4 (BMP4) signaling agonist with a EC50 value of 74 nM, activates BMP signaling by stabilizing intracellular p-SMAD-1/5/9. BMP signaling agonist sb4 activates BMP4 target genes (inhibitors of DNA binding, Id1 and Id3) canonical BMP signaling[1].

IC50 & Target

IC50: BMP4 signal[1]

In Vitro

BMP signaling agonist sb4 (0.05 μM-1 μM; 24 hours) induces phosphorylated SMAD-1/5/9 in a dose-dependent manner in PRECs cells under serum-starved conitions[1].
BMP signaling agonist sb4 (1 μM; 0-60 mins) enhances the efficacy of signaling at each concentration of rhBMP4 tested. This effect is most pronounced at low concentrations of rhBMP4, whereby sb4 increases BRE-luc expression 2-fold at 0.4 ng/ml of rhBMP4[1].

Western Blot Analysis[1]

Cell Line: Primary mouse kidney epithelial cells (PRECs)
Concentration: 0.05 μM-1 μM
Incubation Time: 24 hours
Result: Incresed p-SMAD-1/5/9 abundance in PRECs.

Western Blot Analysis[1]

Cell Line: BRE-Luc cells
Concentration: 1 μM
Incubation Time: 0min, 5 mins, 15 mins, 30 mins, 45 mins, 60 mins
Result: Acted to stabilize p-SMAD-1/5/9 to enhance the transcriptional response.
Molecular Weight

320.20

Formula

C₁₄H₁₀BrNOS

CAS No.

100874-08-6

SMILES

BrC1=CC=C(CSC2=NC3=CC=CC=C3O2)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 125 mg/mL (390.38 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1230 mL 15.6152 mL 31.2305 mL
5 mM 0.6246 mL 3.1230 mL 6.2461 mL
10 mM 0.3123 mL 1.5615 mL 3.1230 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 6.25 mg/mL (19.52 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Keywords:

BMP signaling agonist sb4BMP signaling agonist sb 4BMP signaling agonist sb-4TGF-β ReceptorTransforming growth factor beta receptorsbmpSMADagonistPRECBRE-luccellInhibitorinhibitorinhibit

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Product Name:
BMP signaling agonist sb4
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HY-124697
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