BMP signaling agonist sb4
Based on 8 publication(s) in Google Scholar
BMP signaling agonist sb4 is a potent benzoxazole bone morphogenetic protein 4 (BMP4) signaling agonist with a EC50 value of 74 nM, activates BMP signaling by stabilizing intracellular p-SMAD-1/5/9. BMP signaling agonist sb4 activates BMP4 target genes (inhibitors of DNA binding,?Id1?and?Id3) canonical BMP signaling.
For research use only. We do not sell to patients.
- Purity: 99.98%
- CAS No.: 100874-08-6
- Formula: C14H10BrNOS
- Molecular Weight:320.20
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) BMP signaling agonist sb4
More- Nat Immunol. 2023 Feb;24(2):280-294. [Abstract]
- Nat Commun. 2025 Jul 1;16(1):5470. [Abstract]
- Cell Death Discov. 2024 Nov 14;10(1):468. [Abstract]
- Stem Cell Res Ther. 2023 Oct 26;14(1):305. [Abstract]
- ACS Pharmacol Transl Sci. 2024 Jun 24;7(7):1951-1970. [Abstract]
- FEBS Open Bio. 2025 Sep 10. [Abstract]
- Stem Cells Dev. 2025 Jun;34(11-12):240-248. [Abstract]
- Sci Total Environ. 2022 Dec 1:850:158040. [Abstract]
Customer Validation & Images
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In Vivo Efficacy Study
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RT-PCR
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Cell Proliferation/Viability Assay
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WB
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Apoptosis Analysis
Biological Activity
IC50: BMP4 signal[1]
BMP signaling agonist sb4 (0.05 μM-1 μM; 24 hours) induces phosphorylated SMAD-1/5/9 in a dose-dependent manner in PRECs cells under serum-starved conitions[1]. BMP signaling agonist sb4 (1 μM; 0-60 mins) enhances the efficacy of signaling at each concentration of rhBMP4 tested. This effect is most pronounced at low concentrations of rhBMP4, whereby sb4 increases BRE-luc expression 2-fold at 0.4 ng/ml of rhBMP4[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Primary mouse kidney epithelial cells (PRECs)
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Concentration:0.05 μM-1 μM
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Incubation Time:24 hours
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Result:Increased p-SMAD-1/5/9 abundance in PRECs.
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Cell Line:BRE-Luc cells
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Concentration:1 μM
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Incubation Time:0min, 5 mins, 15 mins, 30 mins, 45 mins, 60 mins
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Result:Acted to stabilize p-SMAD-1/5/9 to enhance the transcriptional response.
Chemical Information
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CAS No. 100874-08-6
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Appearance Solid
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Molecular Weight 320.20
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Formula C14H10BrNOS
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Color White to light yellow
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SMILES
BrC1=CC=C(CSC2=NC3=CC=CC=C3O2)C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (8)
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Journal Impact Factor
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Most Recent
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Nat Immunol
Rebalancing TGFβ1/BMP signals in exhausted T cells unlocks responsiveness to immune checkpoint blockade therapy. [Abstract]2023 Feb;24(2):280-294. PMID: 36543960 -
Nat Commun
Single-cell transcriptomics reveals BMP4-BMPR2 signaling promotes radiation resistance in hematopoietic stem cells following injury. [Abstract]2025 Jul 1;16(1):5470. PMID: 40595466
BMP signaling agonist sb4 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 1;16(1):5470. [Abstract]
Percentage survival of mice after receiving 8.0 Gy of IR and different dosages of SB4 (1, 2.5, 5 mg/kg, i.p.).
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Cell Death Discov
Identification of apelin/APJ signaling dysregulation in a human iPSC-derived granulosa cell model of Turner syndrome. [Abstract]2024 Nov 14;10(1):468. PMID: 39543104
BMP signaling agonist sb4 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2024 Nov 14;10(1):468. [Abstract]
mRNA expression levels of key genes in stem cell pluripotency (differentiation day 0), mesoderm (differentiation day 2), and intermediate mesoderm cells (differentiation day 4–6) by using qRT-PCR analysis under different combinations of SB4 (1, 10 μM) compound and/or BMP4.
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Stem Cell Res Ther
Regulation of self-renewal and senescence in primitive mesenchymal stem cells by Wnt and TGFβ signaling. [Abstract]2023 Oct 26;14(1):305. PMID: 37880755 -
ACS Pharmacol Transl Sci
Bone Morphogenetic Protein Signaling Agonist SB4 (BMPSB4) Inhibits Corticotroph Pituitary Neuroendocrine Tumors by Activation of Autophagy via a BMP4/SMADs-Dependent Pathway. [Abstract]2024 Jun 24;7(7):1951-1970. PMID: 39022361
BMP signaling agonist sb4 purchased from MedChemExpress. Usage Cited in: ACS Pharmacol Transl Sci. 2024 Jun 24;7(7):1951-1970. [Abstract]
Inhibitory effect of BMPSB4 (0, 10, 20, 40, 80 μM,24-96 h) on AtT20/D16v-F2 cells showed dose-dependent and time-dependent effects. Cell viability was detected via the CCK-8 assay.
BMP signaling agonist sb4 purchased from MedChemExpress. Usage Cited in: ACS Pharmacol Transl Sci. 2024 Jun 24;7(7):1951-1970. [Abstract]
Western blot assay showed a concentration-dependent phosphorylation of SMAD1/5/9 by BMPSB4 (40 μM).
BMP signaling agonist sb4 purchased from MedChemExpress. Usage Cited in: ACS Pharmacol Transl Sci. 2024 Jun 24;7(7):1951-1970. [Abstract]
BMPSB4 (40, 80 μM) induced apoptosis of AtT20/D16v-F2 cells and was not affected by NOGGIN.
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FEBS Open Bio
Establishment and optimization of the two-step induction system for generating primordial germ cell-like cells from chicken embryonic stem cells. [Abstract]2025 Sep 10. PMID: 40926636 -
Stem Cells Dev
Compressive Force Inhibits Osteogenic Differentiation of Dental Follicle Stem Cells Through Biglycan/Bone Morphogenetic Protein2/Smad1 Signaling Pathway. [Abstract]2025 Jun;34(11-12):240-248. PMID: 40395190 -
Sci Total Environ
Toxicity mechanisms regulating bone differentiation and development defects following abnormal expressions of miR-30c targeted by triclosan in zebrafish. [Abstract]2022 Dec 1:850:158040. PMID: 35973548
Solvent & Solubility
DMSO : 100 mg/mL (312.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.81 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (7.81 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.1230 mL | 15.6152 mL | 31.2305 mL | 78.0762 mL |
| 5 mM | 0.6246 mL | 3.1230 mL | 6.2461 mL | 15.6152 mL | |
| 10 mM | 0.3123 mL | 1.5615 mL | 3.1230 mL | 7.8076 mL | |
| 15 mM | 0.2082 mL | 1.0410 mL | 2.0820 mL | 5.2051 mL | |
| 20 mM | 0.1562 mL | 0.7808 mL | 1.5615 mL | 3.9038 mL | |
| 25 mM | 0.1249 mL | 0.6246 mL | 1.2492 mL | 3.1230 mL | |
| 30 mM | 0.1041 mL | 0.5205 mL | 1.0410 mL | 2.6025 mL | |
| 40 mM | 0.0781 mL | 0.3904 mL | 0.7808 mL | 1.9519 mL | |
| 50 mM | 0.0625 mL | 0.3123 mL | 0.6246 mL | 1.5615 mL | |
| 60 mM | 0.0521 mL | 0.2603 mL | 0.5205 mL | 1.3013 mL | |
| 80 mM | 0.0390 mL | 0.1952 mL | 0.3904 mL | 0.9760 mL | |
| 100 mM | 0.0312 mL | 0.1562 mL | 0.3123 mL | 0.7808 mL |
BMP signaling agonist sb4 Related Classifications
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