BMP signaling agonist sb4

Name: BMP signaling agonist sb4
Brand: MedChemExpress
SKU: HY-124697
Rating: 4.5 (3 reviews)

Cat. No.: HY-124697 Purity: 99.89%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

BMP signaling agonist sb4 is a potent benzoxazole bone morphogenetic protein 4 (BMP4) signaling agonist with a EC₅₀ value of 74 nM, activates BMP signaling by stabilizing intracellular p-SMAD-1/5/9. BMP signaling agonist sb4 activates BMP4 target genes (inhibitors of DNA binding, Id1 and Id3) canonical BMP signaling.

For research use only. We do not sell to patients.

BMP signaling agonist sb4 Chemical Structure

CAS No. : 100874-08-6

Size	Price	Stock	Quantity

Free Sample (0.1 - 0.5 mg)		Apply Now
Solid + Solvent
10 mM * 1 mL in DMSO ready for reconstitution	USD 55	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	USD 55	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	USD 50	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 70	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 160	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 250	In-stock	Estimated Time of Arrival: December 31
200 mg		Get quote
500 mg		Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

3 Publications Citing Use of MCE BMP signaling agonist sb4

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target

IC50: BMP4 signal^[1]

In Vitro

BMP signaling agonist sb4 (0.05 μM-1 μM; 24 hours) induces? phosphorylated SMAD-1/5/9 in a dose-dependent manner in? PRECs cells under serum-starved conitions^[1].
? BMP signaling agonist sb4 (1 μM; 0-60 mins) enhances the efficacy of signaling at each concentration of rhBMP4 tested. This effect is most pronounced at low concentrations of rhBMP4, whereby sb4 increases BRE-luc expression 2-fold at 0.4 ng/ml of rhBMP4^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:	Primary mouse kidney epithelial cells (PRECs)
Concentration:	0.05 μM-1 μM
Incubation Time:	24 hours
Result:	Increased p-SMAD-1/5/9 abundance in PRECs.

Western Blot Analysis^[1]

Cell Line:	BRE-Luc cells
Concentration:	1 μM
Incubation Time:	0min, 5 mins, 15 mins, 30 mins, 45 mins, 60 mins
Result:	Acted to stabilize p-SMAD-1/5/9 to enhance the transcriptional response.

Molecular Weight

320.20

Formula

C₁₄H₁₀BrNOS

CAS No.

100874-08-6

Appearance

Solid

SMILES

BrC1=CC=C(CSC2=NC3=CC=CC=C3O2)C=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (312.30 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1230 mL	15.6152 mL	31.2305 mL
5 mM	0.6246 mL	3.1230 mL	6.2461 mL
10 mM	0.3123 mL	1.5615 mL	3.1230 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (7.81 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.81 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.89%

Select Batch:

Data Sheet (273 KB) SDS (393 KB)

COA (245 KB) HNMR (244 KB) LCMS (99 KB)

Handling Instructions (2659 KB)

References

[1]. Bradford STJ, et al. High-throughput screens for agonists of bone morphogenetic protein (BMP) signaling identify potent benzoxazole compounds.J Biol Chem. 2019 Mar 1;294(9):3125-3136. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1230 mL	15.6152 mL	31.2305 mL	78.0762 mL
	5 mM	0.6246 mL	3.1230 mL	6.2461 mL	15.6152 mL
	10 mM	0.3123 mL	1.5615 mL	3.1230 mL	7.8076 mL
	15 mM	0.2082 mL	1.0410 mL	2.0820 mL	5.2051 mL
	20 mM	0.1562 mL	0.7808 mL	1.5615 mL	3.9038 mL
	25 mM	0.1249 mL	0.6246 mL	1.2492 mL	3.1230 mL
	30 mM	0.1041 mL	0.5205 mL	1.0410 mL	2.6025 mL
	40 mM	0.0781 mL	0.3904 mL	0.7808 mL	1.9519 mL
	50 mM	0.0625 mL	0.3123 mL	0.6246 mL	1.5615 mL
	60 mM	0.0521 mL	0.2603 mL	0.5205 mL	1.3013 mL
	80 mM	0.0390 mL	0.1952 mL	0.3904 mL	0.9760 mL
	100 mM	0.0312 mL	0.1562 mL	0.3123 mL	0.7808 mL