BSJ-04-122
Based on 3 publication(s) in Google Scholar
BSJ-04-122 is a covalent MKK4/7 dual inhibitor. BSJ-04-122 inhibits MKK4 and MKK7 with IC50 values of 4 nM and 181 nM, respectively. BSJ-04-122 can be used for the research of cancer.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté : 99.88%
- CAS No.: 2513289-74-0
- Formule: C15H12ClN5O
- Masse moléculaire:313.74
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Stockage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) BSJ-04-122
More
Activité biologique
Description
IC50 & Target
IC50: 4 nM (MKK4); 181 nM (MKK7)[1]
In Vitro
BSJ-04-122 has inhibitory activity for MKK4 and MKK7 with IC50 values of 4 nM and 181 nM, respectively[1].
BSJ-04-122 (1, 5, 10 μM; 6 h) induces robust reduction of JNK phosphorylation[1].
BSJ-04-122 (0, 1.25, 2.5, 5, 10, 20 μM; 72 h) shows enhanced antiproliferative effects when combination with JNK-IN-8[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:MDA-MB-231 cells
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Concentration:1, 5, 10 μM
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Incubation Time:6 h
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Result:Significantly decreased levels of T183/Y185 pJNK at 5 μM.
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Cell Line:MDA-MB-231 cells
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Concentration:1-100 μM; 0, 1.25, 2.5, 5, 10, 20 μM
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Incubation Time:72 h
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Result:Exhibited antiproliferative effects when combination with JNK-IN-8 in MDA-MB-231 cells.
Chemical Information
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CAS No. 2513289-74-0
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Appearance Solid
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Masse moléculaire 313.74
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Formule C15H12ClN5O
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Color White to off-white
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SMILES
C=CC(NC1=CC=CC=C1NC2=C3C(NC=C3Cl)=NC=N2)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (3)
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Journal Impact Factor
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Most Recent
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Int J Biol Macromol
Rabies virus matrix protein hijacks the TGF-beta activated kinase 1 binding protein 2-p38 mitogen-activated protein kinase pathway to inhibit apoptosis and promote viral replication. [Abstract]2026 Mar:349:150991. PMID: 41713525 -
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Res Sq
JNK mediates serine phosphorylation of STAT3 in response to fatty acids released by lipolysis. [Abstract]2025 Mar 5:rs.3.rs-6150649. PMID: 40092442
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (318.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (15.94 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocole
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Pureté et documentation
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Fiche technique (285 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.1874 mL | 15.9368 mL | 31.8735 mL | 79.6838 mL |
| 5 mM | 0.6375 mL | 3.1874 mL | 6.3747 mL | 15.9368 mL | |
| 10 mM | 0.3187 mL | 1.5937 mL | 3.1874 mL | 7.9684 mL | |
| 15 mM | 0.2125 mL | 1.0625 mL | 2.1249 mL | 5.3123 mL | |
| 20 mM | 0.1594 mL | 0.7968 mL | 1.5937 mL | 3.9842 mL | |
| 25 mM | 0.1275 mL | 0.6375 mL | 1.2749 mL | 3.1874 mL | |
| 30 mM | 0.1062 mL | 0.5312 mL | 1.0625 mL | 2.6561 mL | |
| 40 mM | 0.0797 mL | 0.3984 mL | 0.7968 mL | 1.9921 mL | |
| 50 mM | 0.0637 mL | 0.3187 mL | 0.6375 mL | 1.5937 mL | |
| 60 mM | 0.0531 mL | 0.2656 mL | 0.5312 mL | 1.3281 mL | |
| 80 mM | 0.0398 mL | 0.1992 mL | 0.3984 mL | 0.9960 mL | |
| 100 mM | 0.0319 mL | 0.1594 mL | 0.3187 mL | 0.7968 mL |
BSJ-04-122 Related Classifications
HY-152185 Related Classifications
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