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Art. -Nr.: HY-184451

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BSS-Et

BSS-Et is a near-infrared phototheranostic agent that serves as one of the two release units of hypoxia-responsive prodrug BNNC, responsible for generating photodynamic therapy (PDT) and photothermal therapy (PTT) effects at tumor sites. BSS-Et produces reactive oxygen species and singlet oxygen upon irradiation, inducing oxidative stress and DNA damage. When combined with (R)-CR8 (HY-18340), BSS-Et enhances DNA damage and apoptosis in breast cancer cells. BSS-Et can be used in breast cancer-related research.

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CAS. Nr.: 2825003-94-7
Formel: C₃₃H₃₁ClN₄O₃
Molecular Weight:567.08

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

BSS-Et Chemical Structure

CAS. Nr.: 2825003-94-7

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- In Vitro
- In Vivo
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
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Biologische Aktivität

Beschreibung

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In Vitro

BSS-Et (30 μM; 1.5 h) induces reactive oxygen species generation in hypoxic 4T1 mouse breast cancer cells upon 808 nm laser irradiation at 1.5 W·cm⁻² for 5 min^[1].
BSS-Et (30 μM; 2 h) reduces viability of hypoxic 4T1 mouse breast cancer cells when combined with 808 nm laser irradiation at 1.5 W·cm⁻² for 5 min^[1].
BSS-Et (30 μM; 2 h) induces DNA double-strand breaks in hypoxic 4T1 mouse breast cancer cells when combined with 808 nm laser irradiation at 1.5 W·cm⁻² for 5 min^[1].
BSS-Et (30 μM; 2 h) reduces mitochondrial membrane potential and induces apoptosis in hypoxic 4T1 mouse breast cancer cells when combined with 808 nm laser irradiation at 1.5 W·cm⁻² for 5 min^[1].
BSS-Et (30 μM; 2 h) induces mitochondrial permeability transition pore opening and apoptosis in hypoxic 4T1 mouse breast cancer cells when combined with 808 nm laser irradiation at 1.5 W·cm⁻² for 5 min^[1].
BSS-Et (30 μM; 2 h) induces apoptosis in 40.2% of hypoxic 4T1 mouse breast cancer cells when combined with 808 nm laser irradiation at 1.5 W·cm⁻² for 5 min^[1].
BSS-Et (30 μM; 2 h) increases γ-H2AX (1.6-fold) and Cleaved Caspase-3 (1.3-fold) expression in hypoxic 4T1 mouse breast cancer cells when combined with 808 nm laser irradiation at 1.5 W·cm⁻² for 5 min, indicating enhanced DNA damage and apoptosis^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

mouse breast cancer 4T1 cells
Concentration:

30 μM
Incubation Time:

2 h; 808 nm laser at 1.5 W·cm^-2 for 5 min, followed by 22 h culture
Result:

Showed a marked increase in red PI fluorescence (dead cells) and decrease in green Calcein-AM fluorescence (live cells) compared to non-irradiated BSS-Et-treated cells.
Exhibited a less pronounced effect than with BNNC + laser treatment.

Immunofluorescence^[1]

Cell Line:

mouse breast cancer 4T1 cells
Concentration:

30 μM
Incubation Time:

2 h; 808 nm laser at 1.5 W·cm^-2 for 5 min, followed by 22 h culture
Result:

Showed pronounced red γ-H2AX fluorescence, indicating DNA double-strand break formation.
Had fluorescence intensity lower than that observed in irradiated BNNC-treated cells but higher than control cells.

Apoptosis Analysis^[1]

Cell Line:

mouse breast cancer 4T1 cells
Concentration:

30 μM
Incubation Time:

2 h; 808 nm laser at 1.5 W·cm^-2 for 5 min, followed by 22 h culture
Result:

Showed a shift from red JC-1 aggregate fluorescence to green JC-1 monomer fluorescence, indicating decreased mitochondrial membrane potential and initiation of apoptosis.
Exhibited a lower magnitude of change compared to irradiated BNNC-treated cells.\nShowed reduced Calcein-AM fluorescence, indicating increased mPTP opening and apoptosis.
Exhibited a lower magnitude of change compared to irradiated BNNC-treated cells.\nShowed 16.5% early apoptotic cells and 23.7% late apoptotic/necrotic cells, totaling 40.2% apoptotic cells.
Had a lower total apoptotic cell percentage than irradiated BNNC-treated cells.

Western Blot Analysis^[1]

Cell Line:

mouse breast cancer 4T1 cells
Concentration:

30 μM
Incubation Time:

2 h; 808 nm laser at 1.5 W·cm^-2 for 5 min
Result:

Showed a 1.6-fold increase in relative γ-H2AX expression compared to control cells.
Showed a 1.3-fold increase in relative Cleaved Caspase-3 expression compared to control cells.
Had lower expression levels than those observed in irradiated BNNC-treated cells.

In Vivo

BSS-Et (30 μM; i.t.; every other day; 21 days) alone does not slow 4T1 breast tumor growth in BALB/c mice, but when combined with 808 nm laser irradiation, it induces significant tumor growth inhibition^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c (female, 7-8 weeks old, subcutaneous 4T1 breast cancer model)^[1]
Dosage:

30 μM
Administration:

i.t.; every other day; 21 days
Result:

Showed rapid tumor progression similar to saline control when administered alone without laser irradiation.
Markedly inhibited tumor expansion when administered with 808 nm laser irradiation, though less potently than the BNNC + laser group.

Chemical Information

CAS. Nr. 2825003-94-7
Molecular Weight 567.08
Formel C₃₃H₃₁ClN₄O₃
SMILES

CC(O/1)(C)C(/C=C/C(CCC/2)=C(Cl)C2=C\C=C3C(C)(C)C(C=C(C(O)=O)C=C4)=C4N\3CC)=C(C#N)C1=C(C#N)\C#N
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Handling Instructions (2659 KB)

Verweise

[1]. Li S, et al. A Hypoxia-Activated Photomolecular Glue with Synergistic Cyclin K Degradation and Phototherapy for Cancer Treatment. Journal of medicinal chemistry. 2026 Jun 25;69(12):14885-14901. [Content Brief]

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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BSS-Et

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