5-Iodouridine
Based on 1 publication(s) in Google Scholar
5-Iodouridine is an iodine-containing pyrimidine nucleoside analog. 5-Iodouridine inhibits dihydroorotase with a Ki value of 340 µM. 5-Iodouridine significantly enhances the cell-killing effect of gamma irradiation. 5-Iodouridine can be used in the research of HSV-1 infection and leukemia.
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- Pureté: 99.93%
- CAS No.: 1024-99-3
- Formule: C9H11IN2O6
- Masse moléculaire:370.10
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Stockage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 5-Iodouridine
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Activité biologique
Description
IC50 & Target
Ki: 340 µM (dihydroorotase)[1]
In Vitro
5-Iodouridine inhibits dihydroorotase, with a Ki of 340 µM[1].
5-Iodouridine (3 mM) significantly enhances the killing effect of gamma-rays on Chinese hamster Don cells under acidic pH (5.4) conditions[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1024-99-3
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Appearance Solid
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Masse moléculaire 370.10
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Formule C9H11IN2O6
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Color White to off-white
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SMILES
O[C@H]1[C@@H](O)[C@H](N2C(NC(C(I)=C2)=O)=O)O[C@@H]1CO
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvant et solubilité
In Vitro:
DMSO : 125 mg/mL (337.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.62 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.62 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (279 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instruction de manipulation (2659 KB)
Références
[2]. Kuroda Y, et al. Radiosensitization of cultured mammalian cells by 5-iodouridine. Int J Radiat Biol Relat Stud Phys Chem Med. 1975 Mar;27(3):247-57. [Content Brief]
[3]. Kumar R, et al. Synthesis and antiviral and cytotoxic activity of iodohydrin and iodomethoxy derivatives of 5-vinyl-2'-deoxyuridines, 2'-fluoro-2'-deoxyuridine, and uridine. J Med Chem. 1990 Feb;33(2):717-23. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7020 mL | 13.5099 mL | 27.0197 mL | 67.5493 mL |
| 5 mM | 0.5404 mL | 2.7020 mL | 5.4039 mL | 13.5099 mL | |
| 10 mM | 0.2702 mL | 1.3510 mL | 2.7020 mL | 6.7549 mL | |
| 15 mM | 0.1801 mL | 0.9007 mL | 1.8013 mL | 4.5033 mL | |
| 20 mM | 0.1351 mL | 0.6755 mL | 1.3510 mL | 3.3775 mL | |
| 25 mM | 0.1081 mL | 0.5404 mL | 1.0808 mL | 2.7020 mL | |
| 30 mM | 0.0901 mL | 0.4503 mL | 0.9007 mL | 2.2516 mL | |
| 40 mM | 0.0675 mL | 0.3377 mL | 0.6755 mL | 1.6887 mL | |
| 50 mM | 0.0540 mL | 0.2702 mL | 0.5404 mL | 1.3510 mL | |
| 60 mM | 0.0450 mL | 0.2252 mL | 0.4503 mL | 1.1258 mL | |
| 80 mM | 0.0338 mL | 0.1689 mL | 0.3377 mL | 0.8444 mL | |
| 100 mM | 0.0270 mL | 0.1351 mL | 0.2702 mL | 0.6755 mL |
5-Iodouridine Related Classifications
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