Cat. No.: HY-16692

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ISO-1

(Synonyms: MIF Antagonist)

25 Cited Publications

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Based on 25 publication(s) in Google Scholar

ISO-1 is a macrophage migration inhibitory factor (MIF) antagonist with an IC₅₀ of 7 μM.

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Pureté: 99.84%
CAS No.: 478336-92-4
Formule: C₁₂H₁₃NO₄
Masse moléculaire:235.24

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

ISO-1 Chemical Structure

CAS No.: 478336-92-4

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (25)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

ISO-1 is a macrophage migration inhibitory factor (MIF) antagonist with an IC₅₀ of 7 μM.

IC₅₀ & Target

IC50: 7 μM (MIF)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
THP-1	IC₅₀	69 μM Compound: iso-1	Inhibition of dopachrome tautomerase activity of MIF in human THP1 cells Inhibition of dopachrome tautomerase activity of MIF in human THP1 cells	[PMID: 19592246]

In Vitro

ISO-1 (0.1-20 μM; 16 hours) has a slight inhibitory effect on Cox-2 secretion without the addition of recombinant MIF^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

RAW 264.7 macrophage cells
Concentration:

0.1μM，1μM，10μM，20μM
Incubation Time:

16 hours
Result:

Suppressed Cox-2 secretion.

In Vivo

ISO-1 (injected intraperitoneally; 3.5-35 mg/kg; twice daily; 2 weeks) improves the survival rate from lethal endotoxemia and shows the anti-inflammatory effect^[2].
ISO-1 (intraperitoneal injection; 35 mg/kg; twice daily; 3 days) causes a significant reduction in average implant size and decreases Flk1 expression in implants^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

C57Bl/6 MIF^+/+ and MIF^–/– mice^[2]
Dosage:

3.5-35 mg/kg
Administration:

Injected intraperitoneally; 3.5-35 mg/kg; twice a day ; 2 weeks
Result:

Was protective against lethal sepsis.

Animal Model:

Female C57BL/6-Tg(ACTB-EGFP)1Osb/J mice^[3]
Dosage:

35 mg/kg
Administration:

Intraperitoneal injection; 35 mg/kg; twice daily; 3 days
Result:

Reduced average endometriotic implant size.

Chemical Information

CAS No. 478336-92-4
Appearance Solid
Masse moléculaire 235.24
Formule C₁₂H₁₃NO₄
Color White to off-white
SMILES

O=C(OC)CC1ON=C(C(C=C2)=CC=C2O)C1
Synonyms

MIF Antagonist
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (25)

All In Vivo ImagingIn Vivo Efficacy StudyHistological Imaging/StainingWB

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Journal Impact Factor

Immunity

Mural cell-derived chemokines provide a protective niche to safeguard vascular macrophages and limit chronic inflammation. [Abstract]

2023 Oct 10;56(10):2325-2341.e15. PMID: 37652021
Acta Pharm Sin B

NO-releasing double-crosslinked responsive hydrogels accelerate the treatment and repair of ischemic stroke. [Abstract]

2025 Feb;15(2):1112-1125. PMID: 40177574
Adv Sci (Weinh)

Pancancer Fine-Mapping of Mutational Intolerance Identifies CHEK1 as an Immunosuppressive Driver in Lung Adenocarcinoma. [Abstract]

2026 May;13(25):e21265. PMID: 41722056
Nat Cardiovasc Res

2024 Apr 25:1-7.
J Neuroinflammation

New insights into meningitic Escherichia coli infection of brain microvascular endothelial cells from quantitative proteomics analysis. [Abstract]

2018 Oct 19;15(1):291. PMID: 30340642

ISO-1 purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2018 Oct 19;15(1):291. [Abstract]

ISO-1 treatment partially recovers PCN033- or RS218-mediated downregulation of the tight junction proteins ZO-1 and occludin.

ISO-1 purchased from MedChemExpress. Usage Cited in: J Neuroinflammation. 2018 Oct 19;15(1):291. [Abstract]

ISO-1 treatment partially recovers PCN033- or RS218-mediated downregulation of the tight junction proteins ZO-1 and occludin.
J Transl Med

Metformin protects against cyclophosphamide-induced ovarian fibrosis by MIF/CD74-mediated macrophage polarization. [Abstract]

2025 Nov 12;23(1):1273. PMID: 41225611
J Invest Dermatol

Dendritic cells remodel eccrine sweat gland niche metabolism through oxidative phosphorylation during aging. [Abstract]

2026 Mar 24:S0022-202X(26)00998-X. PMID: 41887396
J Invest Dermatol

TRPV3-Activated PARP1/AIFM1/MIF Axis through Oxidative Stress Contributes to Atopic Dermatitis. [Abstract]

2024 May 30:S0022-202X(24)00384-1. PMID: 38823435
Inflamm Res

Target Fibroblast-B cell crosstalk via MIF signaling drives pathogenic B cell differentiation and joint damage in knee osteoarthritis. [Abstract]

2025 Dec 17;75(1):4. PMID: 41407905
Mol Ther Oncolytics

Exosome-mediated transfer of MIF confers temozolomide resistance by regulating TIMP3/PI3K/AKT axis in gliomas. [Abstract]

2021 Aug 19:22:114-128. PMID: 34514093

ISO-1 purchased from MedChemExpress. Usage Cited in: Mol Ther Oncolytics. 2021 Aug 19:22:114-128. [Abstract]

U87TR/luciferase cells were orthotopically implanted into the brains of BALB/c mice. DMSO, ISO-1 (5 mg/kg), LY294002, or ISO+LY294002 were injected (i.p.) into the mice twice per week for 28 days. Representative pseudocolor bioluminescence images of orthotopic tumors were obtained.

ISO-1 purchased from MedChemExpress. Usage Cited in: Mol Ther Oncolytics. 2021 Aug 19:22:114-128. [Abstract]

Kaplan-Meier survival curves of mice with different treatments. U87TR/luciferase cells were orthotopically implanted into the brains of BALB/c mice. DMSO, ISO-1 (5 mg/kg), LY294002, or ISO+LY294002 were injected (i.p.) into the mice twice per week for 28 days.

ISO-1 purchased from MedChemExpress. Usage Cited in: Mol Ther Oncolytics. 2021 Aug 19:22:114-128. [Abstract]

Representative H&E staining for tumor cytostructures. U87TR/luciferase cells were orthotopically implanted into the brains of BALB/c mice. DMSO, ISO-1 (5 mg/kg), LY294002, or ISO+LY294002 were injected (i.p.) into the mice twice per week for 28 days.
Commun Biol

MIF downregulation attenuates neuroinflammation via TLR4/MyD88/TRAF6/NF-κB pathway to protect dopaminergic neurons in Parkinson's disease model. [Abstract]

2025 Nov 18;8(1):1595. PMID: 41254084
Inflammation

MIF Regulates the Th17/Treg Ratio to Exacerbate Kidney Injury in Chronic Kidney Disease. [Abstract]

2025 Dec 23;49(1):15. PMID: 41436686
Int Immunopharmacol

Obacunone inhibits RIPK1/RIPK3/MLKL-mediated necroptosis by suppressing mitochondrial ROS and MIF signaling in intestinal ischemia-reperfusion injury. [Abstract]

2026 Jan 1;168(Pt 2):115901. PMID: 41317539
Int Immunopharmacol

MIF regulates T helper 17 cell differentiation by activating the p38 MAPK signaling pathway to drive the pathogenesis of EAP. [Abstract]

2025 May 28:159:114959. PMID: 40440956
Int Immunopharmacol

MIF aggravates experimental autoimmune prostatitis through activation of the NLRP3 inflammasome via the PI3K/AKT pathway. [Abstract]

2024 Aug 16:141:112891. PMID: 39153310
Int Immunopharmacol

MIF inhibitor ISO-1 alleviates severe acute pancreatitis-associated acute kidney injury by suppressing the NLRP3 inflammasome signaling pathway. [Abstract]

2021 Jul:96:107555. PMID: 33823428
Front Cell Dev Biol

Exosomal MIF Derived From Nasopharyngeal Carcinoma Promotes Metastasis by Repressing Ferroptosis of Macrophages. [Abstract]

2021 Dec 31:9:791187. PMID: 35036405
FASEB J

MIF Promotes Phenotypic Switching of VSMCs via AKT/mTOR-Mediated Autophagy Regulation in Aortic Dissection. [Abstract]

2025 Oct 15;39(19):e71079. PMID: 41001812
Exp Cell Res

MIF inhibition attenuates intervertebral disc degeneration by reducing nucleus pulposus cell apoptosis and inflammation. [Abstract]

2024 May 11:114089. PMID: 38740166
Food Chem Toxicol

The crosstalk between M1 macrophage polarization and energy metabolism disorder contributes to polystyrene nanoplastics-triggered testicular inflammation. [Abstract]

2023 Oct:180:114002. PMID: 37634612
Mol Med Rep

Protective effect of ISO‑1 against advanced glycation end product aggravation of PC12 cell injury induced by Aβ1‑40. [Abstract]

2019 Sep;20(3):2135-2142. PMID: 31322215
PLoS One

Human embryonic stem cells secrete macrophage migration inhibitory factor: A novel finding. [Abstract]

2023 Aug 24;18(8):e0288281. PMID: 37616250
bioRxiv

Characterizing the SASP-Dependent Paracrine Spreading of Senescence Between Human Brain Cell Types. [Abstract]

2026 Feb 12:2026.02.10.705129. PMID: 41726875
bioRxiv

2025 Feb 17.
Research Square Preprint

2023 Dec 27

Solvant et solubilité

In Vitro:

Ethanol : 100 mg/mL (425.10 mM; Need ultrasonic)

DMSO : 50 mg/mL (212.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.2510 mL

21.2549 mL

42.5098 mL

5 mM

0.8502 mL

4.2510 mL

8.5020 mL

10 mM

0.4251 mL

2.1255 mL

4.2510 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (10.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (10.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (10.63 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 0.5% CMC-Na/saline water

Solubility: 10 mg/mL (42.51 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Lubetsky JB, et al. The tautomerase active site of macrophage migration inhibitory factor is a potential target for discovery of novel anti-inflammatory agents. J Biol Chem. 2002 Jul 12;277(28):24976-82. [Content Brief]

[2]. Al-Abed Y, et al. ISO-1 binding to the tautomerase active site of MIF inhibits its pro-inflammatory activity and increases survival in severe sepsis. J Biol Chem. 2005 Nov 4;280(44):36541-4. [Content Brief]

[3]. Nothnick WB, et al. Inhibition of macrophage migration inhibitory factor reduces endometriotic implant size in mice with experimentally induced disease. J Endometr. 2011 Sep 30;3(3):135-142. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO / Ethanol	1 mM	4.2510 mL	21.2549 mL	42.5098 mL	106.2744 mL
	5 mM	0.8502 mL	4.2510 mL	8.5020 mL	21.2549 mL
	10 mM	0.4251 mL	2.1255 mL	4.2510 mL	10.6274 mL
	15 mM	0.2834 mL	1.4170 mL	2.8340 mL	7.0850 mL
	20 mM	0.2125 mL	1.0627 mL	2.1255 mL	5.3137 mL
	25 mM	0.1700 mL	0.8502 mL	1.7004 mL	4.2510 mL
	30 mM	0.1417 mL	0.7085 mL	1.4170 mL	3.5425 mL
	40 mM	0.1063 mL	0.5314 mL	1.0627 mL	2.6569 mL
	50 mM	0.0850 mL	0.4251 mL	0.8502 mL	2.1255 mL
	60 mM	0.0708 mL	0.3542 mL	0.7085 mL	1.7712 mL
	80 mM	0.0531 mL	0.2657 mL	0.5314 mL	1.3284 mL
	100 mM	0.0425 mL	0.2125 mL	0.4251 mL	1.0627 mL

Keywords

ISO-1

25 Cited Publications

100 mg Obtenir un devis

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ISO-1

Immunology/Inflammation Compound Library

Clinical Compound Library

Human Metabolite Library

Macrophage Related Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Clinical Compound Library Plus

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Publications Citing Use of MedChemExpress (MCE) ISO-1

Activité biologique

Chemical Information

Publications (25)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

ISO-1

Immunology/Inflammation Compound Library

Clinical Compound Library

Human Metabolite Library

Macrophage Related Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Clinical Compound Library Plus

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Mass Spectrometry Human Metabolite Library

Publications Citing Use of MedChemExpress (MCE) ISO-1

Activité biologique

Chemical Information

Publications (25)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

ISO-1 Related Classifications

HY-16692 Related Classifications