Pilocarpine nitrate
Based on 44 publication(s) in Google Scholar
Pilocarpine nitrate is a potent M3-type muscarinic acetylcholine receptor (M3 muscarinic receptor) agonist.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté: 99.83%
- CAS No.: 148-72-1
- Formule: C11H17N3O5
- Masse moléculaire:271.27
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Stockage:
4°C, sealed storage, away from moisture
* The compound is unstable in solutions, freshly prepared is recommended.
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Pilocarpine nitrate
More- Immunity. 2022 Aug 9;55(8):1466-1482.e9. [Abstract]
- Adv Sci (Weinh). 2026 Jan 28:e19642. [Abstract]
- J Nanobiotechnology. 2026 Apr 21;24(1):546. [Abstract]
- J Nanobiotechnology. 2025 May 5;23(1):332. [Abstract]
- J Nanobiotechnology. 2025 Mar 20;23(1):228. [Abstract]
- Food Chem. 2022 Nov 30:395:133593. [Abstract]
- Acta Pharmacol Sin. 2024 Dec;45(12):2611-2624. [Abstract]
- Cell Rep. 2025 Feb 23;44(3):115343. [Abstract]
- Cell Rep. 2023 Apr 30;42(5):112456. [Abstract]
- Prog Neurobiol. 2022 Oct:217:102335. [Abstract]
- Ocul Surf. 2024 Oct:34:430-440. [Abstract]
- Cells. 2024 Oct 1;13(19):1635. [Abstract]
- Commun Biol. 2024 Sep 17;7(1):1158. [Abstract]
- Life Sci. 2022 Nov 1;308:120942. [Abstract]
- Int J Mol Sci. 2024 Sep 27;25(19):10432. [Abstract]
- Int J Mol Sci. 2023 Apr 16;24(8):7356. [Abstract]
- Int J Mol Sci. 2023 Apr 12;24(8):7171. [Abstract]
- Front Pharmacol. 2023 Jun 26:14:1191692. [Abstract]
- Cell Mol Neurobiol. 2022 Apr;42(3):777-790. [Abstract]
- Int Immunopharmacol. 2024 Feb 15:128:111485. [Abstract]
- Int Immunopharmacol. 2022 Oct:111:109152. [Abstract]
- Neuropharmacology. 2017 May 1:117:238-248. [Abstract]
- Mol Neurobiol. 2025 Dec 1;63(1):232. [Abstract]
- FASEB J. 2024 Aug 15;38(15):e23878. [Abstract]
- J Neurochem. 2025 Dec;169(12):e70334. [Abstract]
- Neurochem Int. 2024 Mar:174:105677. [Abstract]
- Sci Rep. 2025 Jul 15;15(1):25579. [Abstract]
- Cell Signal. 2026 Sep:145:112579. [Abstract]
- Cell Signal. 2026 Aug:144:112553. [Abstract]
- ACS Pharmacol Transl Sci. 2025 Jun 6;8(7):2048-2060. [Abstract]
- Mol Cell Endocrinol. 2025 Jan 21:599:112464. [Abstract]
- Mol Immunol. 2024 Jun 19:172:68-75. [Abstract]
- Epilepsia Open. 2025 Aug 28. [Abstract]
- Brain Sci. 2023 Feb 3;13(2):262. [Abstract]
- Neuroscience. 2021 Feb 10;455:212-222. [Abstract]
- Exp Eye Res. 2024 Jul 16:110008. [Abstract]
- BMC Immunol. 2024 Feb 12;25(1):16. [Abstract]
- J Mol Neurosci. 2020 Nov;70(11):1858-1870. [Abstract]
- PLoS One. 2026 Mar 20;21(3):e0344387. [Abstract]
- Brain Res. 2023 Jul 1:1810:148382. [Abstract]
- Epilepsy Res. 2018 May:142:45-52. [Abstract]
- Research Square Preprint. 2023 Apr 13.
- Research Square Print. December 16th, 2022.
- Research Square Preprint. 2021 Aug.
Customer Validation & Images
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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IF
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Histological Imaging/Staining
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In Vivo Efficacy Study
Activité biologique
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mAChR3 |
To evaluate the cytotoxicity of Pilocarpine, the morphology and viability of human corneal stromal (HCS) cells are examined by light microscopy and MTT assay, respectively. Morphological observations show that HCS cells exposed to Pilocarpine at a concentration from 0.625 to 20 g/L exhibit dose- and time-dependent proliferation retardation and morphological abnormality such as cellular shrinkage, cytoplasmic vacuolation, detachment from culture matrix, and eventually death, while no obvious difference is observed between those exposed to Pilocarpine below the concentration of 0.625 g/L and controls. Results of MTT assay reveal that the cell viability of HCS cells decrease with time and concentration after exposing to Pilocarpine above the concentration of 0.625 g/L (P<0.01 or 0.05), while that of HCS cells treated with Pilocarpine below the concentration of 0.625 g/L show no significant difference to controls[2]. The partial muscarinic agonist, Pilocarpine, evokes concentration-dependent relaxation with an EC50 of 2.4 mM in isolated segments of rat tail artery that were constricted with Penylephrine (10 to 200 nM)[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 148-72-1
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Appearance Solid
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Masse moléculaire 271.27
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Formule C11H17N3O5
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Color White to off-white
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SMILES
[O-][N+](O)=O.O=C1OC[C@H](CC2=CN=CN2C)[C@@H]1CC
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture
* The compound is unstable in solutions, freshly prepared is recommended.
Publications (44)
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Journal Impact Factor
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Most Recent
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Immunity
Microglia-derived PDGFB promotes neuronal potassium currents to suppress basal sympathetic tonicity and limit hypertension. [Abstract]2022 Aug 9;55(8):1466-1482.e9. PMID: 35863346
Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: Immunity. 2022 Aug 9;55(8):1466-1482.e9. [Abstract]
The effects of tamoxifen treatment and microglial PDGFB ablation on pilocarpine (100 mg/kg, ip, once every 20 minutes)-induced epilepsy were investigated by implanting telemetry probes into PDGFBNull-MG mice and analyzing their heart rate (HR) and heart rate variability (HRV) before and 3 or 5 weeks after tamoxifen treatment.
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Adv Sci (Weinh)
Microglial GPR35 Ameliorates Epileptogenesis and Neuroinflammation via PDGFA Domain 2 Signaling. [Abstract]2026 Jan 28:e19642. PMID: 41604576 -
J Nanobiotechnology
Bioengineered anti-EpCAM-guided exosome nanocarriers delivering Foxp3 agonist butyrate achieve tissue-specific immunomodulation in autoimmune Sjögren's syndrome. [Abstract]2026 Apr 21;24(1):546. PMID: 42015251 -
J Nanobiotechnology
Improving epilepsy management by targeting P2 × 7 receptor with ROS/electric responsive nanomicelles. [Abstract]2025 May 5;23(1):332. PMID: 40325469
Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 May 5;23(1):332. [Abstract]
Seizure scores across different groups of the Pilocarpine (300 mg/kg; i.p.)-induced SE model.
Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 May 5;23(1):332. [Abstract]
Survival rate analysis among groups of the Pilocarpine (300 mg/kg; i.p.)-induced SE model.
Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 May 5;23(1):332. [Abstract]
Immunofluorescence staining of CD86 (pro-inflammatory) and CD206 (anti-inflammatory) expression in microglia within the hippocampus across groups of the Pilocarpine (300 mg/kg; i.p.)-induced SE model.
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J Nanobiotechnology
miRNA let-7f-5p-encapsulated labial gland MSC-derived EVs ameliorate experimental Sjögren's syndrome by suppressing Th17 cells via targeting RORC/IL-17A signaling axis. [Abstract]2025 Mar 20;23(1):228. PMID: 40114173 -
Food Chem
Screening of bioactive flavour compounds targeting muscarinic-3 acetylcholine receptor from Siraitia grosvenorii and evaluation of their synergistic anti-asthmatic activity. [Abstract]2022 Nov 30:395:133593. PMID: 35792488 -
Acta Pharmacol Sin
G protein-coupled receptor kinase 2 as a novel therapeutic target for gland fibrosis of Sjögren's syndrome. [Abstract]2024 Dec;45(12):2611-2624. PMID: 39054339 -
Cell Rep
GPNMB and ATP6V1A interact to mediate microglia phagocytosis of multiple types of pathological particles. [Abstract]2025 Feb 23;44(3):115343. PMID: 39992792 -
Cell Rep
The ribosome-associated protein RACK1 represses Kir4.1 translation in astrocytes and influences neuronal activity. [Abstract]2023 Apr 30;42(5):112456. PMID: 37126448
Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: Cell Rep. 2023 Apr 30;42(5):112456. [Abstract]
Latency before the first seizure of PTZ and Pilocarpine ((300 mg/kg; i.p.)-induced seizure tests in RACK1 fl/fl or RACK1 cKO mice.
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Prog Neurobiol
2022 Oct:217:102335. PMID: 35931355
Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: Prog Neurobiol. 2022 Oct:217:102335. [Abstract]
The percentages of Foxp3+ Tregs among CD4+ T cells significantly increased in the hippocampal parenchyma of Pilocarpine(280-300 mg/kg; i.p.)-induced SE mice compared with those in the control group.
Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: Prog Neurobiol. 2022 Oct:217:102335. [Abstract]
GFAP immunostaining in the CA1, CA3, and DG regions of the hippocampi of vehicle-treated control mice, PC61-treated control mice, vehicle-treated Pilocarpine (280-300 mg/kg; i.p.) mice, and PC61-treated Pilocarpine mice.
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Ocul Surf
2024 Oct:34:430-440. PMID: 39343166 -
Cells
2024 Oct 1;13(19):1635. PMID: 39404399
Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: Cells. 2024 Oct 1;13(19):1635. [Abstract]
Mice were intravenously injected with Evans blue or sulfonyl-NHS-biotin tracer, followed by intraperitoneal injection of scopolamine, and finally Pilo (300 mg/kg, ip, single dose) was injected to induce epileptic seizures, with close monitoring. The results indicated increased blood-brain barrier permeability in this Pilo-induced epilepsy model.
Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: Cells. 2024 Oct 1;13(19):1635. [Abstract]
Mice were intravenously injected with Evans blue or sulfonyl-NHS-biotin tracer, followed by intraperitoneal injection of scopolamine, and finally Pilo (300 mg/kg, ip, single dose) was injected to induce epileptic seizures, with close monitoring. The results indicated increased blood-brain barrier permeability in this Pilo-induced epilepsy model.
Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: Cells. 2024 Oct 1;13(19):1635. [Abstract]
Mice were intravenously injected with Evans blue or sulfonyl-NHS-biotin tracer, followed by intraperitoneal injection of scopolamine, and finally Pilo (300 mg/kg, ip, single dose) was injected to induce epileptic seizures, with close monitoring. The results indicated increased blood-brain barrier permeability in this Pilo-induced epilepsy model.
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Commun Biol
An intranasal cationic liposomal polysaccharide vaccine elicits humoral immune responses against Streptococcus pneumoniae. [Abstract]2024 Sep 17;7(1):1158. PMID: 39284859 -
Life Sci
Soluble epoxide hydrolase inhibitor, t-AUCB, improves salivary gland function by ameliorating endothelial injury. [Abstract]2022 Nov 1;308:120942. PMID: 36096247 -
Int J Mol Sci
The Activation of Muscarinic Acetylcholine Receptors Protects against Neuroinflammation in a Mouse Model through Attenuating Microglial Inflammation. [Abstract]2024 Sep 27;25(19):10432. PMID: 39408758 -
Int J Mol Sci
Structural Insights into M1 Muscarinic Acetylcholine Receptor Signaling Bias between Gαq and β-Arrestin through BRET Assays and Molecular Docking. [Abstract]2023 Apr 16;24(8):7356. PMID: 37108518 -
Int J Mol Sci
Immobilization of M3 Muscarinic Receptor to Rapidly Analyze Drug-Protein Interactions and Bioactive Components in a Natural Plant. [Abstract]2023 Apr 12;24(8):7171. PMID: 37108332 -
Front Pharmacol
HECT, UBA and WWE domain containing 1 represses cholesterol efflux during CD4+ T cell activation in Sjögren's syndrome. [Abstract]2023 Jun 26:14:1191692. PMID: 37435494 -
Cell Mol Neurobiol
Expression Pattern of ALOXE3 in Mouse Brain Suggests Its Relationship with Seizure Susceptibility. [Abstract]2022 Apr;42(3):777-790. PMID: 33058074
Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: Cell Mol Neurobiol. 2022 Apr;42(3):777-790. [Abstract]
Pilocarpine (325 mg/kg, ip, single dose)-induced status epilepticus (SE) reduced the level of ALOXE3 protein in the hippocampus of mice, but did not reduce it in the temporal lobe cortex.
Pilocarpine nitrate purchased from MedChemExpress. Usage Cited in: Cell Mol Neurobiol. 2022 Apr;42(3):777-790. [Abstract]
Pilocarpine (325 mg/kg, ip, single dose)-induced status epilepticus (SE) increases AA levels in the hippocampus of mice.
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Int Immunopharmacol
CFL1 restores the migratory capacity of bone marrow mesenchymal stem cells in primary Sjögren's syndrome by regulating CCR1 expression. [Abstract]2024 Feb 15:128:111485. PMID: 38183912 -
Int Immunopharmacol
Mesenchymal stem cell transplantation alleviates Sjögren's syndrome symptoms by modulating Tim-3 expression. [Abstract]2022 Oct:111:109152. PMID: 36007392 -
Neuropharmacology
A novel GLP-1/GIP dual receptor agonist protects from 6-OHDA lesion in a rat model of Parkinson's disease. [Abstract]2017 May 1:117:238-248. PMID: 28223210 -
Mol Neurobiol
2025 Dec 1;63(1):232. PMID: 41324728 -
FASEB J
Pilocarpine mediated excessive calcium accumulation leads to ciliary muscle cell senescence and apoptosis. [Abstract]2024 Aug 15;38(15):e23878. PMID: 39120551 -
J Neurochem
Beta-Arrestin2-Biased Activation by Pilocarpine Suppresses Microglial Inflammatory Response. [Abstract]2025 Dec;169(12):e70334. PMID: 41457680 -
Neurochem Int
H2S inhibits LiCl/pilocarpine-induced seizures and promotes neuroprotection by regulating TRPV2 expression via the AC3/cAMP/PKA pathway. [Abstract]2024 Mar:174:105677. PMID: 38290616 -
Sci Rep
Inhibiting astrocyte calcium dynamics terminates status epilepticus via wireless septal stimulation. [Abstract]2025 Jul 15;15(1):25579. PMID: 40664874 -
Cell Signal
Multi-omics identifies RNF149 as a key molecular marker mediating neuron-glia interaction in temporal lobe epilepsy. [Abstract]2026 Sep:145:112579. PMID: 42114791 -
Cell Signal
Clec7a promotes hippocampal microglial activation in rats with status epilepticus via inducing the TLR4/MyD88/NF-κB signaling pathway. [Abstract]2026 Aug:144:112553. PMID: 42025892 -
ACS Pharmacol Transl Sci
Critical Residues in the Gβ Subunit Mediate Selective Recruitment of the Gαq Heterotrimer to Activated G Protein-Coupled Receptors. [Abstract]2025 Jun 6;8(7):2048-2060. PMID: 40672669 -
Mol Cell Endocrinol
Vagotomy suppresses food intake by increasing GLP-1 secretion via the M3 AChR-AMPKα pathway in mice. [Abstract]2025 Jan 21:599:112464. PMID: 39848433 -
Mol Immunol
MiR-23b-3p alleviates Sjögren's syndrome by targeting SOX6 and inhibiting the NF-κB signaling. [Abstract]2024 Jun 19:172:68-75. PMID: 38901181 -
Epilepsia Open
2025 Aug 28. PMID: 40874679 -
Brain Sci
Microglia PKM2 Mediates Neuroinflammation and Neuron Loss in Mice Epilepsy through the Astrocyte C3-Neuron C3R Signaling Pathway. [Abstract]2023 Feb 3;13(2):262. PMID: 36831807 -
Neuroscience
Rosiglitazone Prevents Autophagy by Regulating Nrf2-Antioxidant Response Element in a Rat Model of Lithium-pilocarpine-induced Status Epilepticus. [Abstract]2021 Feb 10;455:212-222. PMID: 33197503 -
Exp Eye Res
Long-term high fructose intake reprograms the circadian transcriptome and disrupts homeostasis in mouse extra-orbital lacrimal glands. [Abstract]2024 Jul 16:110008. PMID: 39025460 -
BMC Immunol
Network pharmacology-based strategy to investigate the mechanisms of artemisinin in treating primary Sjögren's syndrome. [Abstract]2024 Feb 12;25(1):16. PMID: 38347480 -
J Mol Neurosci
2020 Nov;70(11):1858-1870. PMID: 32430868 -
PLoS One
Hippocampal transcriptome profiling reveals status epilepticus-induced early changes in gene expression mainly implicating in neuroinflammation and immune responses linked to microglial dysfunction. [Abstract]2026 Mar 20;21(3):e0344387. PMID: 41860856 -
Brain Res
(+)-Borneol exerts neuroprotective effects via suppressing the NF-κB pathway in the pilocarpine-induced epileptogenesis rat model. [Abstract]2023 Jul 1:1810:148382. PMID: 37127175 -
Epilepsy Res
Post-treatment with the GLP-1 analogue liraglutide alleviate chronic inflammation and mitochondrial stress induced by Status epilepticus. [Abstract]2018 May:142:45-52. PMID: 29549796 -
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Solvant et solubilité
DMSO : 250 mg/mL (921.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 100 mg/mL (368.64 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.67 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (7.67 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protocole
Cell viability is determined by MTT assay. Briefly, HCS cells are inoculated into a 96-well culture plate (Nunc) at a density of 1×104 cells/100 µL/well, and are cultured and treated. At a 4h interval, the Pilocarpine (0.625 to 20 g/L)-containing medium is replaced entirely with 100 µL serum-free DMEM/F12 medium containing 1.0 g/L MTT, and the cells are incubated at 37°C in the dark for 4h. After the MTT-containing medium is discarded with caution, 150 µL DMSO is added to dissolve the produced formazan crystals at 37°C in the dark for 15 min, and the absorbance at 490 nm is measured with a Multiskan GO microplate reader[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Rats[1]
Male, 10-week-old Wistar rats are assigned to one of two groups, exercise (EX, n=6) and control (CN, n=6). The EX rats are kept for 40 days in cages with a running wheel (SN-451), allowing them to undertake voluntary exercise, while the CN rats are kept in cages with the running wheel locked. On the 40th day, Pilocarpine-induced saliva is measured as follows. Briefly, the rats are anesthetized, preweighed cotton was placed in their mouths sublingually, and Pilocarpine (0.5 mg/kg) is intraperitoneally injected to induce saliva secretion. Each cotton ball is then changed every 10 min for 1 h. The collected cotton balls are weighed again, and the mass of saliva secreted is calculated by subtracting the initial from the final weight.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Pureté et documentation
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Fiche technique (285 KB)
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SDS (479 KB)
- English - EN (479 KB)
- Français - FR (479 KB)
- Deutsch - DE (479 KB)
- Norwegian - NO (479 KB)
- Español - ES (479 KB)
- Swedish - SV (479 KB)
- Italian - IT (479 KB)
- Korean - KR (479 KB)
- Portuguese - PT (479 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Matsuzaki K, et al. Daily voluntary exercise enhances pilocarpine-induced saliva secretion and aquaporin 1 expression in rat submandibular glands. FEBS Open Bio. 2017 Dec 7;8(1):85-93. [Content Brief]
[2]. Yuan XL, et al. Cytotoxicity of pilocarpine to human corneal stromal cells and its underlying cytotoxic mechanisms. Int J Ophthalmol. 2016 Apr 18;9(4):505-11. [Content Brief]
[3]. Tonta MA, et al. Pilocarpine-induced relaxation of rat tail artery by a non-cholinergic mechanism and in the absence of an intact endothelium. Br J Pharmacol. 1994 Jun;112(2):525-32. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.6864 mL | 18.4318 mL | 36.8636 mL | 92.1591 mL |
| 5 mM | 0.7373 mL | 3.6864 mL | 7.3727 mL | 18.4318 mL | |
| 10 mM | 0.3686 mL | 1.8432 mL | 3.6864 mL | 9.2159 mL | |
| 15 mM | 0.2458 mL | 1.2288 mL | 2.4576 mL | 6.1439 mL | |
| 20 mM | 0.1843 mL | 0.9216 mL | 1.8432 mL | 4.6080 mL | |
| 25 mM | 0.1475 mL | 0.7373 mL | 1.4745 mL | 3.6864 mL | |
| 30 mM | 0.1229 mL | 0.6144 mL | 1.2288 mL | 3.0720 mL | |
| 40 mM | 0.0922 mL | 0.4608 mL | 0.9216 mL | 2.3040 mL | |
| 50 mM | 0.0737 mL | 0.3686 mL | 0.7373 mL | 1.8432 mL | |
| 60 mM | 0.0614 mL | 0.3072 mL | 0.6144 mL | 1.5360 mL | |
| 80 mM | 0.0461 mL | 0.2304 mL | 0.4608 mL | 1.1520 mL | |
| 100 mM | 0.0369 mL | 0.1843 mL | 0.3686 mL | 0.9216 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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