Cat. No.: HY-N0708

Vanillic acid

3 Cited Publications

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Based on 3 publication(s) in Google Scholar

Vanillic acid is a flavoring agent found in edible plants and fruits, also found in Angelica sinensis. Vanillic acid inhibits NF-κB activation. Anti-inflammatory, antibacterial, and chemopreventive effects.

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Pureté: 99.43%
CAS No.: 121-34-6
Formule: C₈H₈O₄
Masse moléculaire:168.15

Stockage:

Store at room temperature 3 years.

In solvent -80°C, 2 years , -20°C, 1 year

Vanillic acid Chemical Structure

CAS No.: 121-34-6

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target^[1]

p65	Microbial Metabolite

Voir tous les produits spécifiques à Isoform NF-κB :

Voir tous les produits spécifiques à Isoform Endogenous Metabolite :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>1000 μM Compound: 1b	Anticancer activity against human A549 cells after 24 hrs by MTT assay Anticancer activity against human A549 cells after 24 hrs by MTT assay	[PMID: 20466556]
A549	IC₅₀	>30 μM Compound: 20	Anticancer activity against human A549 cells by SRB assay Anticancer activity against human A549 cells by SRB assay	[PMID: 21420296]
A549	IC₅₀	>100 μM Compound: 16	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 21696954]
A549	IC₅₀	>100 μg/mL Compound: 5	Antiproliferative activity against human A549 cells assessed as growth inhibition after 48 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 26995526]
B16-F10	IC₅₀	>1000 μM Compound: 1b	Anticancer activity against mouse B16F10 cells after 24 hrs by MTT assay Anticancer activity against mouse B16F10 cells after 24 hrs by MTT assay	[PMID: 20466556]
B16-F10	IC₅₀	9.24 mM Compound: Vanillic acid	Cytotoxicity against mouse B16-F10 cells assessed inhibition of cell growth incubated for 24 hrs by MTT assay Cytotoxicity against mouse B16-F10 cells assessed inhibition of cell growth incubated for 24 hrs by MTT assay	[PMID: 36095944]
Hs 683	IC₅₀	>1000 μM Compound: 1b	Anticancer activity against human Hs683 cells after 24 hrs by MTT assay Anticancer activity against human Hs683 cells after 24 hrs by MTT assay	[PMID: 20466556]
HT-22	EC₅₀	27.8 μM Compound: 36	Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay Neuroprotective activity against glutamate-induced cell death in mouse HT-22 cells assessed as increase in cell viability after 24 hrs by EZ-Cytox assay	[PMID: 32991171]
HT-29	IC₅₀	>100 μg/mL Compound: 5	Antiproliferative activity against human HT-29 cells assessed as growth inhibition after 48 hrs by MTT assay Antiproliferative activity against human HT-29 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 26995526]
HUVEC	IC₅₀	>100 μg/mL Compound: 5	Antiproliferative activity against human HUVEC cells assessed as growth inhibition after 48 hrs by MTT assay Antiproliferative activity against human HUVEC cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 26995526]
LoVo	IC₅₀	>1000 μM Compound: 1b	Anticancer activity against human LoVo cells after 24 hrs by MTT assay Anticancer activity against human LoVo cells after 24 hrs by MTT assay	[PMID: 20466556]
LoVo	IC₅₀	91 μM Compound: 16	Cytotoxicity against human LoVo cells after 72 hrs by MTT assay Cytotoxicity against human LoVo cells after 72 hrs by MTT assay	[PMID: 21696954]
MCF7	IC₅₀	>1000 μM Compound: 1b	Anticancer activity against human MCF7 cells after 24 hrs by MTT assay Anticancer activity against human MCF7 cells after 24 hrs by MTT assay	[PMID: 20466556]
MCF7	IC₅₀	>100 μg/mL Compound: 5	Antiproliferative activity against human MCF7 cells assessed as growth inhibition after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as growth inhibition after 48 hrs by MTT assay	[PMID: 26995526]
OE33	IC₅₀	>1000 μM Compound: 1b	Anticancer activity against human OE33 cells after 24 hrs by MTT assay Anticancer activity against human OE33 cells after 24 hrs by MTT assay	[PMID: 20466556]
PC-3	IC₅₀	>1000 μM Compound: 1b	Anticancer activity against human PC3 cells after 24 hrs by MTT assay Anticancer activity against human PC3 cells after 24 hrs by MTT assay	[PMID: 20466556]
PC-3	IC₅₀	>100 μM Compound: 16	Cytotoxicity against human PC3 cells after 72 hrs by MTT assay Cytotoxicity against human PC3 cells after 72 hrs by MTT assay	[PMID: 21696954]
RAW264.7	IC₅₀	23.2 μM Compound: 9	Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in LPS-induced NO production after 24 hrs by Griess assay Antiinflammatory activity in mouse RAW264.7 cells assessed as decrease in LPS-induced NO production after 24 hrs by Griess assay	[PMID: 25666824]
SK-MEL-2	IC₅₀	>30 μM Compound: 20	Anticancer activity against human SK-MEL-2 cells by SRB assay Anticancer activity against human SK-MEL-2 cells by SRB assay	[PMID: 21420296]
SK-MEL-28	IC₅₀	>100 μM Compound: 16	Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay Cytotoxicity against human SK-MEL-28 cells after 72 hrs by MTT assay	[PMID: 21696954]
SK-OV-3	IC₅₀	>30 μM Compound: 20	Anticancer activity against human SKOV3 cells by SRB assay Anticancer activity against human SKOV3 cells by SRB assay	[PMID: 21420296]
T98G	IC₅₀	>1000 μM Compound: 1b	Anticancer activity against human T98G cells after 24 hrs by MTT assay Anticancer activity against human T98G cells after 24 hrs by MTT assay	[PMID: 20466556]
U-373MG ATCC	IC₅₀	>1000 μM Compound: 1b	Anticancer activity against human U373 cells after 24 hrs by MTT assay Anticancer activity against human U373 cells after 24 hrs by MTT assay	[PMID: 20466556]
U-373MG ATCC	IC₅₀	>100 μM Compound: 16	Cytotoxicity against human U373 cells after 72 hrs by MTT assay Cytotoxicity against human U373 cells after 72 hrs by MTT assay	[PMID: 21696954]
XF498	IC₅₀	>30 μM Compound: 20	Anticancer activity against human XF498 cells by SRB assay Anticancer activity against human XF498 cells by SRB assay	[PMID: 21420296]

In Vitro

Vanillic acid is non-toxic to HT22 cells at all three concentrations (50, 100 and 200?μM); Vanillic acid co-treatment with Aβ_1-42 significantly increases (1.5-, 1.9- and 2-fold respectively) cell viability^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

HT22 cells
Concentration:

50, 100 and 200 μM
Incubation Time:

24 hours
Result:

Increased the viability of HT22 cells after 24 h at three different concentrations (50, 100 and 200 μM).

In Vivo

Vanillic acid (3-30 mg/kg; i.p.; 5 hours) inhibits Carrageenan-induced mechanical hyperalgesia, paw edema, and neutrophil and macrophage recruitment^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Swiss mice (25-30 g)^[1]
Dosage:

3, 10, or 30 mg/kg
Administration:

Intraperitoneal treatment; 5 hours
Result:

Inhibited Carrageenan-induced mechanical hyperalgesia, paw edema, and neutrophil and macrophage recruitment.

Chemical Information

CAS No. 121-34-6
Appearance Solid
Masse moléculaire 168.15
Formule C₈H₈O₄
Color Off-white to light brown
SMILES

O=C(O)C1=CC=C(O)C(OC)=C1
Structure Classification
- Phenols
- Monophenols
- Ketones, Aldehydes, Acids
Initial Source
- Plants
- Umbelliferae
- Microorganisms
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Publications (3)

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Journal Impact Factor

Antioxidants (Basel)

Bioactive Extracts and Constituents from Taraxacum mongolicum: Antioxidant, Anti-Inflammatory, Enzyme-Inhibitory, and Molecular Docking Studies. [Abstract]

2026 May 29;15(6):688. PMID: 42351994
Food Funct

Blackthorn juice from central Italy exhibits selective anticancer activity by inducing apoptosis and autophagy in gastric carcinoma cells. [Abstract]

2025 Oct 27;16(21):8548-8563. PMID: 41085359
Mol Nutr Food Res

Therapeutic Potential of Vanillic Acid in Ulcerative Colitis Through Microbiota and Macrophage Modulation. [Abstract]

2025 Feb;69(4):e202400785. PMID: 39812000

Solvant et solubilité

In Vitro:

DMSO : ≥ 100 mg/mL (594.71 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.9471 mL

29.7357 mL

59.4714 mL

5 mM

1.1894 mL

5.9471 mL

11.8943 mL

10 mM

0.5947 mL

2.9736 mL

5.9471 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (14.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (14.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (14.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (276 KB)
SDS (254 KB)
- English - EN (254 KB)
- Français - FR (254 KB)
- Deutsch - DE (254 KB)
- Norwegian - NO (254 KB)
- Español - ES (254 KB)
- Swedish - SV (254 KB)
- Italian - IT (254 KB)
- Korean - KR (254 KB)
- Portuguese - PT (254 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Calixto-Campos C, et al. Vanillic acid Inhibits Inflammatory Pain by Inhibiting Neutrophil Recruitment, Oxidative Stress, Cytokine Production, and NFκB Activation in Mice. J Nat Prod. 2015 Aug 28;78(8):1799-808. [Content Brief]

[2]. Amin FU, et al. Vanillic acid attenuates Aβ_1-42-induced oxidative stress and cognitive impairment in mice. Sci Rep. 2017 Jan 18;7:40753. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.9471 mL	29.7357 mL	59.4714 mL	148.6785 mL
	5 mM	1.1894 mL	5.9471 mL	11.8943 mL	29.7357 mL
	10 mM	0.5947 mL	2.9736 mL	5.9471 mL	14.8679 mL
	15 mM	0.3965 mL	1.9824 mL	3.9648 mL	9.9119 mL
	20 mM	0.2974 mL	1.4868 mL	2.9736 mL	7.4339 mL
	25 mM	0.2379 mL	1.1894 mL	2.3789 mL	5.9471 mL
	30 mM	0.1982 mL	0.9912 mL	1.9824 mL	4.9560 mL
	40 mM	0.1487 mL	0.7434 mL	1.4868 mL	3.7170 mL
	50 mM	0.1189 mL	0.5947 mL	1.1894 mL	2.9736 mL
	60 mM	0.0991 mL	0.4956 mL	0.9912 mL	2.4780 mL
	80 mM	0.0743 mL	0.3717 mL	0.7434 mL	1.8585 mL
	100 mM	0.0595 mL	0.2974 mL	0.5947 mL	1.4868 mL

Keywords

Vanillic acid

3 Cited Publications

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Vanillic acid

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Publications Citing Use of MedChemExpress (MCE) Vanillic acid

Voir tous les produits spécifiques à Isoform NF-κB

Voir tous les produits spécifiques à Isoform Endogenous Metabolite

Activité biologique

Chemical Information

Publications (3)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table