AY 9944
Based on 10 publication(s) in Google Scholar
AY 9944 is a specific cholesterol biosynthesis inhibitor. AY 9944 inhibits the 7-dehydro cholesterol Δ7-reductase (DHCR7) enzyme with an IC50 of 13 nM. AY 9944 causes hypocholesterolemia and accumulation of 7DHC. At high doses, AY 9944 inhibits also in cultured embryos sterol Δ7-Δ8 isomerase, which causes the accumulation of cholest-8-en-3β-ol.
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- Pureté: 99.67%
- CAS No.: 366-93-8
- Formule: C22H30Cl4N2
- Masse moléculaire:464.30
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Stockage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) AY 9944
More- Adv Sci (Weinh). 2026 Jun 19:e76165. [Abstract]
- Cancer Lett. 2024 Mar 1:584:216609. [Abstract]
- EMBO Mol Med. 2025 Dec;17(12):3586-3606. [Abstract]
- Mol Syst Biol. 2025 Nov 13. [Abstract]
- Antiviral Res. 2023 Jan:209:105497. [Abstract]
- Toxicon. 2025 Apr 17:260:108366. [Abstract]
- Biochem Biophys Res Commun. 2025 Nov 29:794:153070. [Abstract]
- Uppsala University. 2025.
- bioRxiv. 2025 March 19.
- Uppsala University. 2024 Jul 12.
Customer Validation & Images
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Cell Proliferation/Viability Assay
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Histological Imaging/Staining
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IF
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IF
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Activité biologique
IC50: 13 nM (DHCR7)1
AY 9944 (1 μg/mL; 15 h) affects types of free sterol in keratinocytes[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human foreskin keratinocytes
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Concentration:1 μg/mL
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Incubation Time:15 hours
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Result:Inhibited the synthesis of cholesterol and desmosterol, but increased 7-dehydrocholesterol and zymosterol synthesis.
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.
AY 9944 (7.5 mg/kg; i.h. every 6 days once from postnatal day 2 to postnatal day 20) reduces brain cholesterol until 400 days after stop treatment[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Long–Evans hooded rats[3].
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Dosage:7.5 mg/kg
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Administration:Subcutaneous injection; 7.5 mg/kg every 6 days once; from postnatal day 2 to postnatal day 20
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Result:Reduced brain cholesterol and increased 7-dehydrocholesterol in all brain regions of rats till the treatment stopped for 400 days and showed a more severe effect on the change of sterols in plasma and liver of female rats.
Chemical Information
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CAS No. 366-93-8
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Appearance Solid
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Masse moléculaire 464.30
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Formule C22H30Cl4N2
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Color White to off-white
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SMILES
ClC(C=CC=C1)=C1CNC[C@H](CC2)CC[C@@H]2CNCC3=C(C=CC=C3)Cl.[H]Cl.[H]Cl
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture)
Publications (10)
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Journal Impact Factor
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Most Recent
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Adv Sci (Weinh)
Targeting the HSPA8-CMA-ATP6V1A Axis Triggers Lysosomal Hyperacidification and Catastrophic Vacuolation in Prostate Cancer. [Abstract]2026 Jun 19:e76165. PMID: 42318659 -
Cancer Lett
DHCR7 promotes lymph node metastasis in cervical cancer through cholesterol reprogramming-mediated activation of the KANK4/PI3K/AKT axis and VEGF-C secretion. [Abstract]2024 Mar 1:584:216609. PMID: 38211648
AY 9944 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2024 Mar 1:584:216609. [Abstract]
AY 9944 (7.5 mg/kg, every other day). Representative images of H&E staining of popliteal lymph nodes indicating tumor cell infiltration. The red arrows indicate the part of tumor cell infiltration.
AY 9944 purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2024 Mar 1:584:216609. [Abstract]
AY 9944 (7.5 mg/kg, every other day). LYVE-1 IF staining showed lymphatic vascular density in footpad tumors of the mock, AY9944, and TAM groups in mouse models.
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EMBO Mol Med
Inhibiting cholesterol synthesis halts rhabdomyosarcoma growth via ER stress and cell cycle arrest. [Abstract]2025 Dec;17(12):3586-3606. PMID: 41249736
AY 9944 purchased from MedChemExpress. Usage Cited in: EMBO Mol Med. 2025 Dec;17(12):3586-3606. [Abstract]
RD cells were treated with different concentrations of the DHCR7 inhibitor (AY9944), and their growth curves were analyzed by live-cell imaging.
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Mol Syst Biol
Spatial proteomics of ovarian cancer precursors delineates early disease changes and drug targets. [Abstract]2025 Nov 13. PMID: 41233595 -
Antiviral Res
Targeting 7-dehydrocholesterol reductase against EV-A71 replication by upregulating interferon response. [Abstract]2023 Jan:209:105497. PMID: 36528172
AY 9944 purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2023 Jan:209:105497. [Abstract]
Pretreatment with AY9944 (15, 30 µM), U18666A, lovastatin and MβCD for 2 h can all significantly reduce the level of EV-A71 VP1 protein in HCT-8 cell.
AY 9944 purchased from MedChemExpress. Usage Cited in: Antiviral Res. 2023 Jan:209:105497. [Abstract]
AY9944 (30 μМ; 2 h) significantly inhibits the expression of EV-A71 VP1 protein in HCT-8 and RD cells.
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Toxicon
The DHCR7 is the key target of lipotoxic liver injury caused by matrine through abnormal activation of the cholesterol synthesis pathway. [Abstract]2025 Apr 17:260:108366. PMID: 40250732 -
Biochem Biophys Res Commun
7-dehydrocholesterol promotes CCL2-secretion from porcine skeletal muscle satellite cells, enhancing the migration of preadipocytes. [Abstract]2025 Nov 29:794:153070. PMID: 41344212 -
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Solvant et solubilité
DMSO : 5 mg/mL (10.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 5 mg/mL (10.77 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (274 KB)
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SDS (480 KB)
- English - EN (480 KB)
- Français - FR (480 KB)
- Deutsch - DE (480 KB)
- Norwegian - NO (480 KB)
- Español - ES (480 KB)
- Swedish - SV (480 KB)
- Italian - IT (480 KB)
- Korean - KR (480 KB)
- Portuguese - PT (480 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Moebius FF, et al. Molecular cloning and expression of the human delta7-sterol reductase. Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1899-902. [Content Brief]
[2]. Nemanic MK, et al. In vitro synthesis of vitamin D-3 by cultured human keratinocytes and fibroblasts: action spectrum and effect of AY-9944. Biochim Biophys Acta. 1985 Sep 6;841(3):267-77. [Content Brief]
[3]. Cortez MA, et al. Brain sterols in the AY-9944 rat model of atypical absence seizures. Epilepsia. 2002 Jan;43(1):3-8. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 2.1538 mL | 10.7689 mL | 21.5378 mL | 53.8445 mL |
| 5 mM | 0.4308 mL | 2.1538 mL | 4.3076 mL | 10.7689 mL | |
| 10 mM | 0.2154 mL | 1.0769 mL | 2.1538 mL | 5.3844 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
AY 9944 Related Classifications
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