EphB1-IN-1
Based on 1 publication(s) in Google Scholar
EphB1-IN-1 (Compound 1) is a potent EphB1 inhibitor with IC50s of 3.0, 15 and 220 nM against EphB1G703C, EphB1T697G and EphB1WT, respectively.
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- Pureté : 99.51%
- CAS No.: 1980036-18-7
- Formule: C16H12Cl2N4O2
- Masse moléculaire:363.20
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Stockage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) EphB1-IN-1
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Activité biologique
Description
IC50 & Target
IC50: 3.0 nM (EphB1G703C), 15 nM (EphB1T697G), 220 nM (EphB1WT)[1]
In Vitro
EphB1-IN-1 (Compound 1) and 3MB-PP1 (HY-102069) cause orthogonal inhibition of two Eph kinases in the same cell[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HEK293 cells
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Concentration:300 nM
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Incubation Time:
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Result:Inhibited EphB1G703C. Abolished the autophosphorylation of EphB3WT with little effect on that of EphB1T697G. 100 nM of 3MB-PP1 abolished the autophosphorylation of EphB1T697G but had little effect on that of EphB3WT.
Chemical Information
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CAS No. 1980036-18-7
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Appearance Solid
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Masse moléculaire 363.20
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Formule C16H12Cl2N4O2
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Color White to off-white
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SMILES
O=C(NC1=CC2=NC=NC(NC3=CC(O)=CC=C3Cl)=C2C=C1)CCl
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvant et solubilité
In Vitro:
DMSO : 150 mg/mL (413.00 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 3.75 mg/mL (10.32 mM); Clear solution
This protocol yields a clear solution of ≥ 3.75 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (37.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (272 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.7533 mL | 13.7665 mL | 27.5330 mL | 68.8326 mL |
| 5 mM | 0.5507 mL | 2.7533 mL | 5.5066 mL | 13.7665 mL | |
| 10 mM | 0.2753 mL | 1.3767 mL | 2.7533 mL | 6.8833 mL | |
| 15 mM | 0.1836 mL | 0.9178 mL | 1.8355 mL | 4.5888 mL | |
| 20 mM | 0.1377 mL | 0.6883 mL | 1.3767 mL | 3.4416 mL | |
| 25 mM | 0.1101 mL | 0.5507 mL | 1.1013 mL | 2.7533 mL | |
| 30 mM | 0.0918 mL | 0.4589 mL | 0.9178 mL | 2.2944 mL | |
| 40 mM | 0.0688 mL | 0.3442 mL | 0.6883 mL | 1.7208 mL | |
| 50 mM | 0.0551 mL | 0.2753 mL | 0.5507 mL | 1.3767 mL | |
| 60 mM | 0.0459 mL | 0.2294 mL | 0.4589 mL | 1.1472 mL | |
| 80 mM | 0.0344 mL | 0.1721 mL | 0.3442 mL | 0.8604 mL | |
| 100 mM | 0.0275 mL | 0.1377 mL | 0.2753 mL | 0.6883 mL |
EphB1-IN-1 Related Classifications
HY-115481 Related Classifications
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