NCI-B16
Based on 1 Customer Validation
NCI-B16 is a small molecule RNA binder obtained through RNA-small molecule interaction screening. NCI-B16 interacts with RNA motifs under competitive conditions. NCI-B16 is used for RNA-small molecule interaction studies.
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- Pureté : 99.25%
- CAS No.: 802835-01-4
- Formule: C27H26N8O4
- Masse moléculaire:526.55
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Stockage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
Description
In Vitro
NCI-B16 remains among 91 unique RNA-binding small molecules after competitive screening with a 1,000-fold excess of bulk tRNA to exclude non-selective RNA motif binders[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 802835-01-4
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Appearance Solid
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Masse moléculaire 526.55
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Formule C27H26N8O4
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Color White to off-white
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SMILES
O=C(C1=CC(NC(NC2=CC=C(C3=NCCN3)C=C2)=O)=CC(NC(NC4=CC=C(C5=NCCN5)C=C4)=O)=C1)O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvant et solubilité
In Vitro:
DMSO : 100 mg/mL (189.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocole
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Protocol for Yeast Two-Hybrid (Y2H) Assay
The yeast two-hybrid assay detects binary protein-protein interactions by separating a transcription factor into a DNA-binding domain fused to a "bait" protein and a transcriptional activation domain fused to a "prey" protein; if bait and prey interact in yeast, the transcription factor is reconstituted and activates reporter genes such as HIS3, ADE2, lacZ, MEL1, or other selectable/readable reporters. The readout is yeast growth on selective medium and/or reporter activity, which reflects proximity-dependent transcriptional activation in the yeast nucleus rather than direct biochemical binding in the original mammalian, tumor, neuronal, macrophage, or organoid context. Because yeast two-hybrid can generate false positives and false negatives, interaction claims should be validated using independent assays such as co-immunoprecipitation, Western blot, immunofluorescence colocalization, BiFC, pull-down, or mammalian two-hybrid assays.
Pureté et documentation
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Fiche technique (276 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8992 mL | 9.4958 mL | 18.9915 mL | 47.4789 mL |
| 5 mM | 0.3798 mL | 1.8992 mL | 3.7983 mL | 9.4958 mL | |
| 10 mM | 0.1899 mL | 0.9496 mL | 1.8992 mL | 4.7479 mL | |
| 15 mM | 0.1266 mL | 0.6331 mL | 1.2661 mL | 3.1653 mL | |
| 20 mM | 0.0950 mL | 0.4748 mL | 0.9496 mL | 2.3739 mL | |
| 25 mM | 0.0760 mL | 0.3798 mL | 0.7597 mL | 1.8992 mL | |
| 30 mM | 0.0633 mL | 0.3165 mL | 0.6331 mL | 1.5826 mL | |
| 40 mM | 0.0475 mL | 0.2374 mL | 0.4748 mL | 1.1870 mL | |
| 50 mM | 0.0380 mL | 0.1899 mL | 0.3798 mL | 0.9496 mL | |
| 60 mM | 0.0317 mL | 0.1583 mL | 0.3165 mL | 0.7913 mL | |
| 80 mM | 0.0237 mL | 0.1187 mL | 0.2374 mL | 0.5935 mL | |
| 100 mM | 0.0190 mL | 0.0950 mL | 0.1899 mL | 0.4748 mL |
NCI-B16 Related Classifications
HY-156215 Related Classifications
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Keywords
- NCI-B16
- 802835-01-4
- DNA/RNA Synthesis
- HCV RNA stem-loop SL I
- antisense oligonucleotide
- HCV RNA stem-loop SL II
- SGR-Neo-RLuc-JFH1-2A replicon cells
- subgenomic replicon cells
- HCV 3' UTR stem-loop II RNA motif
- hepatitis C virus
- RNase H-mediated cleavage
- HCV 3' UTR stem-loop I RNA motif
- HCV RNA replication
- Inhibitor
- inhibitor
- inhibit