NHI-2
Based on 1 Customer Validation
NHI-2 is a lactate dehydrogenase A (LDHA) inhibitor with an IC50 of 14.7 µM. NHI-2 shows selective for LDHA over LDHB (IC50 = 55.8 µM). NHI-2 is an efficient anti-glycolytic agent. NHI-2 enhances apoptosis, induces cell cycle arrest at S and G2 phases. NHI-2 has a broad spectrum anti-proliferative activity in cancer cells. NHI-2 affects extracellular acidification rate and ATP production. NHI-2 suppresses tumor growth in murine B78 melanoma tumor model.
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- Pureté : 99.10%
- CAS No.: 1269802-97-2
- Formule: C17H12F3NO3
- Masse moléculaire:335.28
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Stockage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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GalloflavinLactate Dehydrogenase Inhibitor -
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IsomaltProtein-Stabilizing Excipient/Sweetener -
HY-139319
LDHA-IN-3LDHA Inhibitor
Activité biologique
Description
In Vitro
NHI-2 (50-200 μM, 8 h) inhibits lactate production in HeLa cells[1].
NHI-2 (12-40 μM, 72 h) inhibits proliferation in B78 cells[2].
NHI-2 (0.1-100 μM, 48 h) has an EC50 of 32 μM against B78 cells[2].
NHI-2 (40 μM, 24 h) increases cell apoptosis when combined with either 30 or 60 mM Oxamate (Oxa) (HY-W013032A)[2].
NHI-2 (40 μM, 24 h) induces cell cycle arrest at S/G2 phases[2].
NHI-2 affects extracellular acidification rate (ECAR) (12-26 μM, 80 min) and ATP (18 μM, 24 h) production, and has a synergistic effect with Oxa[2].
NHI-2 acts as NADH competitor, not
only affects glycolysis but also impacts mitochondrial metabolism and the NAD+/NADH
balance[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:B78-D14 melanoma cells
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Concentration:0.1-100 μM
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Incubation Time:48 h
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Result:The EC50 values of 32 μM.
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Cell Line:B78-D14 melanoma cells
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Concentration:12, 18, 26, 40, μM
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Incubation Time:72 h
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Result:Exhibited no effect on B78 cell proliferation at lower concentrations. but decreased cell proliferation at concentrations beginning from 26 μM.
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Cell Line:B78-D14 melanoma cells
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Concentration:40 μM
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Incubation Time:24 h
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Result:Increased cell apoptosis when combined with either 30 or 60 mM Oxa.
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Cell Line:B78-D14 melanoma cells
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Concentration:40 μM
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Incubation Time:24 h
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Result:Led to an increased cell population in the S and G2 phases.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 female mice (6-8 weeks) were intradermally implanted with B78 syngeneic melanoma cells[2]
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Dosage:0.9 mg/kg
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Administration:Intra-tumoral (IT) via percutaneous needle puncture; once daily for 15 days
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Result:Suppressed tumor volume when combined with checkpoint inhibitors (ICIs).
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Flow cytometric DNA-content cell-cycle staining
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Patient-Derived Xenograft (PDX)
- Apoptosis Solutions
- Patient-Derived Orthotopic Xenograft (PDOX)
- Protocol for Cell Cycle
- Subcutaneous Cell-Line-Derived Xenograft
- Orthotopic Cell-Line Xenograft
- Cell Viability Determination by MTT Colorimetric Assay
Chemical Information
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CAS No. 1269802-97-2
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Appearance Solid
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Masse moléculaire 335.28
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Formule C17H12F3NO3
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Color White to off-white
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SMILES
ON1C2=CC(C3=CC=CC=C3)=CC(C(F)(F)F)=C2C=C1C(OC)=O
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvant et solubilité
In Vitro:
DMSO : 25 mg/mL (74.56 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Pureté et documentation
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Fiche technique (279 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9826 mL | 14.9129 mL | 29.8258 mL | 74.5645 mL |
| 5 mM | 0.5965 mL | 2.9826 mL | 5.9652 mL | 14.9129 mL | |
| 10 mM | 0.2983 mL | 1.4913 mL | 2.9826 mL | 7.4565 mL | |
| 15 mM | 0.1988 mL | 0.9942 mL | 1.9884 mL | 4.9710 mL | |
| 20 mM | 0.1491 mL | 0.7456 mL | 1.4913 mL | 3.7282 mL | |
| 25 mM | 0.1193 mL | 0.5965 mL | 1.1930 mL | 2.9826 mL | |
| 30 mM | 0.0994 mL | 0.4971 mL | 0.9942 mL | 2.4855 mL | |
| 40 mM | 0.0746 mL | 0.3728 mL | 0.7456 mL | 1.8641 mL | |
| 50 mM | 0.0597 mL | 0.2983 mL | 0.5965 mL | 1.4913 mL | |
| 60 mM | 0.0497 mL | 0.2485 mL | 0.4971 mL | 1.2427 mL |
NHI-2 Related Classifications
HY-124309 Related Classifications
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