Paridiprubart (WT IgG1)
Based on 1 Customer Validation
Paridiprubart (WT IgG1) (NI-0101) is a humanised anti-TLR4 monoclonal antibody. Paridiprubart (WT IgG1) inhibits the production of pro-inflammatory cytokines by monocytes from rheumatoid arthritis specimen induced by rheumatoid arthritis synovial fluid (RASF). Paridiprubart (WT IgG1) can be used for the study of rheumatoid arthritis.
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- Pureté : ≥95.0%
- CAS No.: 2641646-59-3
- Masse moléculaire:145.16 kDa
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Stockage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Activité biologique
Description
Isotype
Human IgG1 kappa
Recommend Isotype Controls
Species Reactivity
Human
IC50 & Target
TLR4[1]
In Vitro
Paridiprubart (WT IgG1) (20 μg/mL, pre-treated for 30 minutes) significantly inhibits the production of pro-inflammatory cytokines by monocytes from rheumatoid arthritis specimen induced by rheumatoid arthritis synovial fluid (RASF)[1].
Paridiprubart (WT IgG1) inhibits the production of TNF-α by macrophages stimulated by citrullinated fibrinogen immune complexes (cFb-IC) through an Fc-dependent mechanism[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Monocytes from rheumatoid arthritis specimen
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Concentration:20 μg/mL
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Incubation Time:pre-treated for 30 minutes
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Result:Reduced the production of pro-inflammatory cytokines such as IL-6, TNFα, IL-1β and IL-8.
Essai clinique
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Human IgG1 kappa
Application
ELISA, FACS, Functional assay
Verified Bioactivity
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Flow cytometric analysis of 1X106 Jurkat cells with Paridiprubart (HY-145645, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
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CAS No. 2641646-59-3
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Appearance Liquid
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Masse moléculaire 145.16 kDa
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Color Colorless to light yellow
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SMILES
[Paridiprubart (WT IgG1)]
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Synonyms
NI-0101
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Livraison
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Stockage
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureté et documentation
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Fiche technique (269 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
Références
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)