Cat. No.: HY-B1584

Captan

Name: Captan
Brand: MedChemExpress
SKU: HY-B1584
Price: 25.00 USD
Availability: InStock
Rating: 5 (1 reviews)

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Captan is a thiol-reactive fungicide. Captan irreversibly depletes protein and non-protein thiols (including glutathione), reducing enzyme activity and fungal respiration/metabolism. Captan inhibits DNA synthesis and replication fork progression, and induces ROS accumulation, oxidative stress, inflammation, ER stress, mitochondrial dysfunction, autophagy, DNA damage, and apoptosis as well as loss of membrane integrity with necrosis. Captan is used to control fungal diseases of various fruits, vegetables, and ornamental plants.

For research use only. We do not sell to patients.

Purity : 99.61%
CAS No.: 133-06-2
Formula: C₉H₈Cl₃NO₂S
Molecular Weight:300.59

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Captan Chemical Structure

CAS No.: 133-06-2

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

Anti-cancerApoptosisCell Cycle RegulationFungal InfectionOxidative StressOthers

Anti-cancer

Captan (0.2-1 µM; 66-90 h post-transfection) reduces clonogenic survival in HeLa cells when XRCC1 is depleted^[2].
Captan (1-20 µM; 2 h) induces γH2AX and 53BP1 DNA damage responses in HeLa cells, with a γH2AX-positive/53BP1-negative subpopulation^[2].
Captan (20 µM; 2 h) activates replicative-stress markers pCHK1 and FANCD2 monoubiquitylation in HeLa cells^[2].
Captan (10 µM; 2 h) induces 53BP1 foci in HeLa cells, with a higher proportion after ATR depletion^[2].

Apoptosis

Captan (1-30 μM; 3 h) is cytotoxic to rat thymic lymphocytes at 10 and 30 μM, and at 30 μM also induces cell shrinkage and increased side scatter^[4].
Captan (10-30 μM; 1 h; 0.5 h annexin V-FITC/PI) induces annexin V-positive living rat thymic lymphocytes before cell death at 30 μM, consistent with an apoptotic process^[4].

Cell Cycle Regulation

Captan (10 µM; 2 h) induces S-phase γH2AX in AA8 and EM9 CHO cells independently of XRCC1 status^[2].
Captan (10 µM; 2 h; EdU 5 µM for 15 min) impairs EdU incorporation during S phase in AA8 and EM9 CHO cells independently of XRCC1 status^[2].

Fungal Infection

Captan (20-40 μM; 6-72 h) is highly toxic to aerobic Saccharomyces cerevisiae BY4741, with 20 μM causing 92.0% CFU reduction after 6 h^[1].
Captan (20 μM; 6 h) causes loss of membrane integrity in most Saccharomyces cerevisiae BY4741 cells, consistent with necrotic death^[1].

Fungal Infection

Apoptosis

Captan (20 μM) induces apoptosis in a subset of Saccharomyces cerevisiae BY4741 cells, in addition to the predominant necrotic phenotype^[1].

Oxidative Stress

Captan (6 h) depletes both protein and non-protein thiol pools in Saccharomyces cerevisiae BY4741 in a dose-dependent manner^[1].
Captan (1-30 μM; 1.5 h total) lowers cellular nonprotein thiol content in rat thymic lymphocytes at 10 and 30 μM^[4].
Captan (10-30 μM; 1.5 h) increases superoxide anion levels in rat thymic lymphocytes at 10 and 30 μM^[4].
Captan (3-10 μM; 3 h) increases the cytotoxicity of 300 μM H₂O₂ toward rat thymic lymphocytes at 3 and 10 μM^[4].

Oxidative Stress

Fungal Infection

Captan (20 μM; 6 h) toxicity to Saccharomyces cerevisiae BY4741 was protected against by thiol-containing compounds cysteine and glutathione, but not ascorbic acid^[1].
Captan (20 μM) exposure increases intracellular ROS in Saccharomyces cerevisiae BY4741^[1].

Others

Captan (0.2-1 µM; 6-12 days) reduces clonogenic survival more strongly in XRCC1-deficient EM9 CHO cells than in AA8 CHO cells^[2].
Captan (1-10 µM; 24 h) increases micronucleus formation in CHO cells, with greater induction in XRCC1-deficient EM9 cells^[2].
Captan (1 µM; 2 h; 0-60 min recovery) induces Fpg-sensitive base lesions in AA8 and EM9 CHO cells whose repair requires XRCC1^[2].
Captan (1 µM; 2 h) increases alkaline comet DNA damage in human PBMC^[2].
Captan (1-30 μM; 1 h) raises intracellular Ca²⁺ in rat thymic lymphocytes at 10 and 30 μM by increasing membrane Ca²⁺ permeability dependent on external Ca²⁺^[4].
Captan (1-30 μM; 1 h) raises intracellular Zn²⁺ in rat thymic lymphocytes at 10 and 30 μM by increasing intracellular Zn²⁺ release independently of external Zn²⁺^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

HeLa cells transfected with control or XRCC1 siRNA
Concentration:

0.2, 0.5, and 1 µM
Incubation Time:

66-90 h post-transfection
Result:

XRCC1-depleted HeLa cells showed lower clonogenic survival than control siRNA cells; at 1 µM, XRCC1-depleted cells were more sensitive than controls.

Immunofluorescence^[2]

Cell Line:

HeLa cells
Concentration:

1, 10, and 20 µM
Incubation Time:

2 h
Result:

A dose-dependent increase in both γH2AX-positive and 53BP1-positive HeLa cells was observed at 1-20 µM.
A subclass of cells accumulated γH2AX without 53BP1 foci.

Western Blot Analysis^[2]

Cell Line:

HeLa cells
Concentration:

20 µM
Incubation Time:

2 h
Result:

CHK1 phosphorylation at S345 and FANCD2 monoubiquitylation were increased compared with non-treated cells.

Cell Viability Assay^[2]

Cell Line:

HeLa cells transfected with control or ATR siRNA
Concentration:

0.2, 0.5, and 1 µM
Incubation Time:

66-90 h post-transfection
Result:

ATR-depleted HeLa cells showed lower clonogenic survival than control siRNA cells, with reduced viability at 1 µM.

Immunofluorescence^[2]

Cell Line:

HeLa cells transfected with control or ATR siRNA
Concentration:

10 µM
Incubation Time:

2 h
Result:

The proportion of 53BP1-positive cells increased; the proportion was higher after ATR depletion than in control siRNA cells.

In Vivo

Cytokines & ChemokinesEndoplasmic Reticulum StressApoptosisAutophagyOxidative StressMitochondrial Function

Cytokines & Chemokines

Endoplasmic Reticulum Stress

Apoptosis

Autophagy

Oxidative Stress

Mitochondrial Function

Captan (20-100 mg/kg/day; oral gavage; 28 days) exposure impairs ovarian homeostasis and oocyte quality in female Kunming mice, accompanied by inflammation, ER stress, apoptosis, autophagy, oxidative stress, DNA damage, and mitochondrial dysfunction^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Chemical Information

CAS No. 133-06-2
Appearance Solid
Molecular Weight 300.59
Formula C₉H₈Cl₃NO₂S
Color Off-white to light yellow
SMILES

O=C1N(SC(Cl)(Cl)Cl)C(C2CC=CCC12)=O
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (166.34 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.3268 mL

16.6340 mL

33.2679 mL

5 mM

0.6654 mL

3.3268 mL

6.6536 mL

10 mM

0.3327 mL

1.6634 mL

3.3268 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

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Molecular Weight Molecular Weight

Dilution Calculator

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
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g
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (327 KB)
SDS (949 KB)
- English - EN (949 KB)
- Français - FR (949 KB)
- Deutsch - DE (949 KB)
- Norwegian - NO (949 KB)
- Español - ES (949 KB)
- Swedish - SV (949 KB)
- Italian - IT (949 KB)
- Korean - KR (949 KB)
- Portuguese - PT (949 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Scariot FJ, et al. Necrotic and apoptotic cell death induced by Captan on Saccharomyces cerevisiae. World journal of microbiology & biotechnology. 2017 Aug;33(8):159.

[2]. Fernandez-Vidal A, et al. Exposure to the Fungicide Captan Induces DNA Base Alterations and Replicative Stress in Mammalian Cells. Environmental and molecular mutagenesis. 2019 Apr;60(3):286-297.

[3]. He QK, et al. Captan exposure disrupts ovarian homeostasis and affects oocytes quality via mitochondrial dysfunction induced apoptosis. Chemosphere. 2022 Jan;286(Pt 1):131625.

[4]. Inoue T, et al. Captan-induced increase in the concentrations of intracellular Ca2+ and Zn2+ and its correlation with oxidative stress in rat thymic lymphocytes. Environ Toxicol Pharmacol. 2018 Oct;63:78-83.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.3268 mL	16.6340 mL	33.2679 mL	83.1698 mL
	5 mM	0.6654 mL	3.3268 mL	6.6536 mL	16.6340 mL
	10 mM	0.3327 mL	1.6634 mL	3.3268 mL	8.3170 mL
	15 mM	0.2218 mL	1.1089 mL	2.2179 mL	5.5447 mL
	20 mM	0.1663 mL	0.8317 mL	1.6634 mL	4.1585 mL
	25 mM	0.1331 mL	0.6654 mL	1.3307 mL	3.3268 mL
	30 mM	0.1109 mL	0.5545 mL	1.1089 mL	2.7723 mL
	40 mM	0.0832 mL	0.4158 mL	0.8317 mL	2.0792 mL
	50 mM	0.0665 mL	0.3327 mL	0.6654 mL	1.6634 mL
	60 mM	0.0554 mL	0.2772 mL	0.5545 mL	1.3862 mL
	80 mM	0.0416 mL	0.2079 mL	0.4158 mL	1.0396 mL
	100 mM	0.0333 mL	0.1663 mL	0.3327 mL	0.8317 mL

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Captan