Casein hydrolysate
Based on 1 Customer Validation
Casein hydrolysate is an orally effective, multi-functional mixture of bioactive peptides, a hydrolysis product of casein (HY-125865), containing ACE inhibitors and immunomodulatory target regulators, among others. Casein hydrolysate releases short-chain bioactive peptides through enzymatic hydrolysis of casein, increasing cellular glutathione and catalase levels, enhancing ConA-induced IL-2 production, and exhibiting metal ion chelation, free radical scavenging, antibacterial, and mineral absorption-promoting effects. Casein hydrolysate lowers blood pressure by inhibiting angiotensin-converting enzyme (ACE) and reducing bradykinin degradation.
For research use only. We do not sell to patients.
- CAS No.: 65072-00-6
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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IL-2 |
Casein hydrolysate inhibits lipid peroxidation at concentrations of 0.05-0.5 mg/mL, and can chelate Fe2+, scavenging peroxyl radicals and hydroxyl radicals[1].
The IC50 value of casein hydrolysate (0-50% (v/v); 24 h) on Jurkat T cells ranges from 19.5%-66.8% (v/v), and does not affect cell membrane integrity at concentrations ≤1% (v/v)[2].
Casein hydrolysate (0.05% (v/v); 24 h) significantly increased GSH content in Jurkat cells in antioxidant experiments, but did not affect superoxide dismutase (SOD) activity[2].
Casein hydrolysate (0.05% (v/v); 24 h) neither produced genotoxicity nor protected cells from H2O2-induced DNA damage in Caco-2 cell comet assays[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Human Jurkat T cells
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Concentration:0%, 0.05%, 0.10%, 0.25%, 0.50%, 1.3%, 2.5%, 5.0%, 10.0%, 25.0%, 50.0% (v/v)
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Incubation Time:
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Result:Exerted concentration-dependent effects on the viability of Jurkat T cells after 24 hours of treatment. The IC50 values ranged from 19.5% to 66.8% (v/v) (protein-based IC50: 1%-5.1% w/v), with hydrolysates f and g showing the lowest IC50.
Hydrolysates a, c, and d did not induce 50% cell death, and no significant increase in LDH release was observed at concentrations ≤1% (v/v), indicating no impairment of cell membrane integrity.
Casein hydrolysate-derived peptide Leu-Lys-Pro-Asp-Meth (100 μg/kg; i.v.; single dose; immediately after administration) reduces blood pressure by 30 mmHg in spontaneously hypertensive rats (SHR), and its hydrolysis product Leu-Lys-Pro (30 μg/kg; intravenous injection; single dose; immediately after administration) reduces blood pressure by 50 mmHg[1].
Casein phosphopeptides (CPP) from casein hydrolysate inhibits bone resorption, stimulates bone mineralization, and promotes calcium integration into bone tissue in a decalcified bone matrix rat model[1].
Casein hydrolysate-related immunomodulatory peptide-derived IgY prevents bacterial adhesion to the intestinal epithelium and dose-dependently prevents enterotoxigenic E. coli infection in a colostrum-deficient piglet model of enterotoxigenic E. coli infection[1].
Casein hydrolysate stimulates the production of superoxide anions by leukocytes and enhances non-specific immune defense in an Atlantic salmon (Salmo salar) immunostimulation model[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Spontaneously Hypertensive Rats (SHR) hypertension model & Normotensive rats normal blood pressure model[1]
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Dosage:Ile-Pro-Pro/Val-Pro-Pro peptides: ≤5 mg/kg; 10 mg/kg for normotensive rats
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Administration:Single oral administration
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Result:At doses up to 5 mg/kg significantly decreased systolic blood pressure in SHR 6 to 8 hours post-administration.
A dose of 10 mg/kg showed no effect on systolic blood pressure in normotensive rats, indicating dose-dependent and model-specific antihypertensive activity.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 65072-00-6
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Appearance Solid
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Color Off-white to light yellow
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SMILES
[Casein hydrolysate]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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