Art. -Nr.: HY-113279

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7-Dehydrocholesterol

8 Cited Publications

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Based on 8 publication(s) in Google Scholar

7-Dehydrocholesterol is biosynthetic precursor of cholesterol and vitamin D₃.

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Reinheit: 99.97%
CAS. Nr.: 434-16-2
Formel: C₂₇H₄₄O
Molecular Weight:384.64

Speicherung:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

7-Dehydrocholesterol Chemical Structure

CAS. Nr.: 434-16-2

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (8)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

7-Dehydrocholesterol is biosynthetic precursor of cholesterol and vitamin D₃.

IC₅₀ & Target

Human Endogenous Metabolite

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

In Vitro

7-Dehydrocholesterol is a biosynthetic precursor of cholesterol and vitamin D₃. 7-Dehydrocholesterol is present in relatively high concentration in skin where it is converted to pre-vitamin D₃ upon UV irradiation and where it is also exposed to exogenous radical sources and oxygen^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 434-16-2
Appearance Solid
Molecular Weight 384.64
Formel C₂₇H₄₄O
Color White to light yellow
SMILES

CC(C)CCC[C@@H](C)[C@H]1CC[C@@]2([H])C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C
Structure Classification
- Steroids
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (8)

All Cell Imaging/StainingCell Proliferation/Viability AssayWB

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Journal Impact Factor

Adv Sci (Weinh)

Fe-S Protein FDX1 Triggers Tumor-Intrinsic Innate Immunity via Mitochondrial Nucleic Acids Release to Orchestrate Ferroptosis in CCRCC. [Abstract]

2025 Nov 7:e18323. PMID: 41199656
Chem Eng J

2025 Aug 1.
Cell Chem Biol

Transient hydroxycholesterol treatment restrains TCR signaling to promote long-term immunity. [Abstract]

2024 May 16;31(5):920-931.e6. PMID: 38759618

7-Dehydrocholesterol purchased from MedChemExpress. Usage Cited in: Cell Chem Biol. 2024 May 16;31(5):920-931.e6. [Abstract]

Mouse naive CD8+ T cells were transiently treated with 1 or 2 ng/μL 7-Dehydrocholesterol (7a-HC) in A and 2 ng/μL 7a-HC for 20 min.

7-Dehydrocholesterol purchased from MedChemExpress. Usage Cited in: Cell Chem Biol. 2024 May 16;31(5):920-931.e6. [Abstract]

Effects of transient 7-Dehydrocholesterol (7a-HC) treatment on the phosphorylation levels of TCR and downstream molecules in mouse T cells. Mouse naive CD4+ T cells were transiently treated with 2 ng/μL 7a-HC for 20 min and then stimulated with 5 ng/μL α-CD3ε and 5 ng/μL α-CD28.

7-Dehydrocholesterol purchased from MedChemExpress. Usage Cited in: Cell Chem Biol. 2024 May 16;31(5):920-931.e6. [Abstract]

Naïve mouse CD8+ T cells were treated with 10 ng/μL cholesterol, 2 ng/μL 7-Dehydrocholesterol (7a-HC) for 20 min, or without any treatment. The plasma membrane was then labeled with octadecylrhodamine B (R18).
Anal Chim Acta

2026 Jun 16;1418:345855.
Virol Sin

Antiviral activity of vitamin D derivatives against severe fever with thrombocytopenia syndrome virus in vitro and in vivo. [Abstract]

2024 Oct;39(5):802-811. PMID: 39168248
Insect Biochem Mol Biol

Interaction of ML1 and SRB1-12 mediates cholesterol transport in the brown planthopper. [Abstract]

2026 Mar:188:104499. PMID: 41548698
Biochem Biophys Res Commun

7-dehydrocholesterol promotes CCL2-secretion from porcine skeletal muscle satellite cells, enhancing the migration of preadipocytes. [Abstract]

2026 Jan 1:794:153070. PMID: 41344212

7-Dehydrocholesterol purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2026 Jan 1:794:153070. [Abstract]

Cell viability of MuSCs with different concentrations of 7-Dehydrocholesterol (7-DHC).

7-Dehydrocholesterol purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2026 Jan 1:794:153070. [Abstract]

The protein level of CCL2 in MuSCs after pre-treatment of MuSCs with 7-Dehydrocholesterol (7-DHC) (in a co-culture system).
Research Square Preprint

2023 Jun 22.

Lösungsmittel & Löslichkeit

In Vitro:

Ethanol : 12.5 mg/mL (32.50 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5998 mL

12.9992 mL

25.9983 mL

5 mM

0.5200 mL

2.5998 mL

5.1997 mL

10 mM

0.2600 mL

1.2999 mL

2.5998 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 0.83 mg/mL (2.16 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% EtOH 90% Corn Oil

Solubility: ≥ 0.83 mg/mL (2.16 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (8.3 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (272 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Xu L, et al. Oxysterols from free radical chain oxidation of 7-dehydrocholesterol: product and mechanistic studies. J Am Chem Soc. 2010 Feb 24;132(7):2222-32. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
Ethanol	1 mM	2.5998 mL	12.9992 mL	25.9983 mL	64.9958 mL
	5 mM	0.5200 mL	2.5998 mL	5.1997 mL	12.9992 mL
	10 mM	0.2600 mL	1.2999 mL	2.5998 mL	6.4996 mL
	15 mM	0.1733 mL	0.8666 mL	1.7332 mL	4.3331 mL
	20 mM	0.1300 mL	0.6500 mL	1.2999 mL	3.2498 mL
	25 mM	0.1040 mL	0.5200 mL	1.0399 mL	2.5998 mL
	30 mM	0.0867 mL	0.4333 mL	0.8666 mL	2.1665 mL

Keywords

7-Dehydrocholesterol

8 Cited Publications

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7-Dehydrocholesterol

Lipid Compound Library

Traditional Chinese Medicine Active Compound Library

Gut Microbial Metabolite Library

Microbial Metabolite Library

Food-Sourced Compound Library

Human Metabolite Library

Animal-Sourced Natural Product Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Bioactive Compound Library

PBCRBS Traditional Chinese Medicine Compound Library

Vitamin Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Endogenous Metabolite Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Mass Spectrometry Human Metabolite Library

Mouse Metabolite Compound Library

Cosmetic Ingredient Compound Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Lipid Metabolite Compound Library

Natural Product Diversity Scaffold Library

Mass Spectrometry Natural Product Library

Natural Product and Natural Product-Like Compound Library

Metabolism/Protease Compound Library

Natural Product Library

Human Endogenous Metabolite Compound Library

Publications Citing Use of MedChemExpress (MCE) 7-Dehydrocholesterol

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (8)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

7-Dehydrocholesterol

Lipid Compound Library

Traditional Chinese Medicine Active Compound Library

Gut Microbial Metabolite Library

Microbial Metabolite Library

Food-Sourced Compound Library

Human Metabolite Library

Animal-Sourced Natural Product Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Bioactive Compound Library

PBCRBS Traditional Chinese Medicine Compound Library

Vitamin Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Mass Spectrometry Human Endogenous Metabolite Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Mass Spectrometry Human Metabolite Library

Mouse Metabolite Compound Library

Cosmetic Ingredient Compound Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Lipid Metabolite Compound Library

Natural Product Diversity Scaffold Library

Mass Spectrometry Natural Product Library

Natural Product and Natural Product-Like Compound Library

Metabolism/Protease Compound Library

Natural Product Library

Human Endogenous Metabolite Compound Library

Publications Citing Use of MedChemExpress (MCE) 7-Dehydrocholesterol

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (8)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

7-Dehydrocholesterol Related Classifications

HY-113279 Related Classifications