Art. -Nr.: HY-N0840

Bruceantin

(Synonyms: (-)-Bruceantin; NCI165563; NSC165563)

2 Cited Publications

Kundenbewertung

Based on 2 publication(s) in Google Scholar

Bruceantin ((-)-Bruceantin) is a quassinoid found in B. javanica. Bruceantin activates caspase signaling pathway, causes the mitochondrial dysfunction, inhibits cell proliferation, induces cell differentiation and apoptosis. Bruceantin exhibits anti-leukemia and antiprotozoal activities.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

Reinheit: 99.67%
CAS. Nr.: 41451-75-6
Formel: C₂₈H₃₆O₁₁
Molecular Weight:548.58

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Bruceantin Chemical Structure

CAS. Nr.: 41451-75-6

Größe

Preis

Verfügbarkeit

Menge

Amount: USD 0.00

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Alle Caspase Isoform-spezifische Produkte anzeigen :

Alle Parasite Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
BV-173	IC₅₀	<15 ng/mL Compound: Bruceantin	Cytotoxicity against human BV173 cells by trypan blue exclusion method Cytotoxicity against human BV173 cells by trypan blue exclusion method	[PMID: 14987068]
Daudi	IC₅₀	<15 ng/mL Compound: Bruceantin	Cytotoxicity against human Daudi cells by trypan blue exclusion method Cytotoxicity against human Daudi cells by trypan blue exclusion method	[PMID: 14987068]
HeLa	IC₅₀	0.029 μM Compound: 9; BRC	Antiproliferative activity against human HeLa cells Antiproliferative activity against human HeLa cells	[PMID: 33289552]
HeLa	IC₅₀	0.051 μM Compound: 7	Inhibition of protein synthesis in human HeLa cells assessed as [35S]methionine incorporation into protein treated for 45 mins before [35S]methionine addition by Whatman paper analysis Inhibition of protein synthesis in human HeLa cells assessed as [35S]methionine incorporation into protein treated for 45 mins before [35S]methionine addition by Whatman paper analysis	[PMID: 19199792]
HL-60	EC₅₀	0.02 μM Compound: 2	Induction of cell differentiation in human HL60 cells assessed as superoxide anion production after 4 days by NBT reduction assay Induction of cell differentiation in human HL60 cells assessed as superoxide anion production after 4 days by NBT reduction assay	[PMID: 11754601]
HL-60	IC₅₀	0.04 μM Compound: 2	Antiproliferative activity against human HL60 cells assessed as inhibition of [3H]thymidine incorporation after 4 days by liquid scintillation counter Antiproliferative activity against human HL60 cells assessed as inhibition of [3H]thymidine incorporation after 4 days by liquid scintillation counter	[PMID: 11754601]
HL-60	IC₅₀	0.04 μM Compound: 2	Cytotoxicity against human HL60 cells assessed as loss of membrane integrity after 4 days by trypan blue exclusion assay Cytotoxicity against human HL60 cells assessed as loss of membrane integrity after 4 days by trypan blue exclusion assay	[PMID: 11754601]
HL-60	IC₅₀	12.2 nM Compound: Bruceantin	Induction of apoptosis in human HL60 cells after 24 hrs by DAPI staining Induction of apoptosis in human HL60 cells after 24 hrs by DAPI staining	[PMID: 14987068]
HL-60	IC₅₀	20 ng/mL Compound: Bruceantin	Cytotoxicity against human HL60 cells by trypan blue exclusion method Cytotoxicity against human HL60 cells by trypan blue exclusion method	[PMID: 14987068]
HT-29	IC₅₀	0.022 μM Compound: 9; BRC	Antiproliferative activity against human HT-29 cells Antiproliferative activity against human HT-29 cells	[PMID: 33289552]
Kasumi 1	IC₅₀	20 ng/mL Compound: Bruceantin	Cytotoxicity against human Kasumi-1 cells by trypan blue exclusion method Cytotoxicity against human Kasumi-1 cells by trypan blue exclusion method	[PMID: 14987068]
KB	ED₅₀	17 μg/mL Compound: Bruceantine	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 7130986]
LNCaP	ED₅₀	11 nM Compound: 6	Cytotoxicity against hormone-dependent human LNCAP cells after 2 days by sulforhodamine B assay Cytotoxicity against hormone-dependent human LNCAP cells after 2 days by sulforhodamine B assay	[PMID: 19026551]
Lu1	ED₅₀	12 nM Compound: 6	Cytotoxicity against human Lu1 cells after 2 days by sulforhodamine B assay Cytotoxicity against human Lu1 cells after 2 days by sulforhodamine B assay	[PMID: 19026551]
MCF7	ED₅₀	0.27 nM Compound: 6	Cytotoxicity against human MCF7 cells after 2 days by sulforhodamine B assay in presence of 43 uM (-)-hydnocarpin Cytotoxicity against human MCF7 cells after 2 days by sulforhodamine B assay in presence of 43 uM (-)-hydnocarpin	[PMID: 19026551]
MCF7	ED₅₀	2 nM Compound: 6	Cytotoxicity against human MCF7 cells after 2 days by sulforhodamine B assay Cytotoxicity against human MCF7 cells after 2 days by sulforhodamine B assay	[PMID: 19026551]
NB-4	IC₅₀	<15 ng/mL Compound: Bruceantin	Cytotoxicity against human NB4 cells by trypan blue exclusion method Cytotoxicity against human NB4 cells by trypan blue exclusion method	[PMID: 14987068]
NCI-H929	IC₅₀	115 nM Compound: Bruceantin	Induction of apoptosis in human NCI-H929 cells after 24 hrs by DAPI staining Induction of apoptosis in human NCI-H929 cells after 24 hrs by DAPI staining	[PMID: 14987068]
REH	IC₅₀	20 ng/mL Compound: Bruceantin	Cytotoxicity against human REH cells by trypan blue exclusion method Cytotoxicity against human REH cells by trypan blue exclusion method	[PMID: 14987068]
RPMI-8226	IC₅₀	12.8 nM Compound: Bruceantin	Induction of apoptosis in human RPMI8226 cells after 24 hrs by DAPI staining Induction of apoptosis in human RPMI8226 cells after 24 hrs by DAPI staining	[PMID: 14987068]
RS4-11	IC₅₀	<15 ng/mL Compound: Bruceantin	Cytotoxicity against human RS4:11 cells by trypan blue exclusion method Cytotoxicity against human RS4:11 cells by trypan blue exclusion method	[PMID: 14987068]
SU-DHL-6	IC₅₀	<15 ng/mL Compound: Bruceantin	Cytotoxicity against human DHL6 cells by trypan blue exclusion method Cytotoxicity against human DHL6 cells by trypan blue exclusion method	[PMID: 14987068]
U-266	IC₅₀	49 nM Compound: Bruceantin	Induction of apoptosis in human U266 cells after 24 hrs by DAPI staining Induction of apoptosis in human U266 cells after 24 hrs by DAPI staining	[PMID: 14987068]
U-937	IC₅₀	<15 ng/mL Compound: Bruceantin	Cytotoxicity against human U937 cells by trypan blue exclusion method Cytotoxicity against human U937 cells by trypan blue exclusion method	[PMID: 14987068]

In Vitro

Bruceantin (2.5-40 ng/mL, 24 h) downregulates the expression of c-Myc in cell RPMI 8226, induces a decrease in mitochondrial membrane potential, leads to the release of cytochrome c, activates caspase-3/7, and induces apoptosis in cells RPMI 8226, U266 and H929 with IC₅₀s of 13, 49 and 115 nM, respectively^[1].
Bruceantin (0.1-1 μg/mL, 4-5 days) exhibits anti-amoebic activity, inhibits Entamoeba histolytica with an IC₅₀ of 0.018 μg/mL^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

RPMI 8226, U266 and H929
Concentration:

2.5-40 ng/mL
Incubation Time:

24 h
Result:

Induced apoptosis.

Western Blot Analysis^[1]

Cell Line:

RPMI 8226
Concentration:

2.5-40 ng/mL
Incubation Time:

24 h
Result:

Inhibited the expression of c-Myc in RPMI 8226.

In Vivo

Bruceantin (1.25-12 mg/kg, ip, every 3 days for 40 days) exhibits antitumor effect in mouse RPMI 8226 xenograft models^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Mouse RPMI 8226 xenograft models^[1]
Dosage:

1.25-12 mg/kg
Administration:

ip, every 3 days for 40 days
Result:

Inhibited tumor growth.

Chemical Information

CAS. Nr. 41451-75-6
Appearance Solid
Molecular Weight 548.58
Formel C₂₈H₃₆O₁₁
Color White to off-white
SMILES

O=C([C@@]1([C@H]([C@H](O)[C@]2([H])[C@]3(C4)C)O)[C@@]([C@H](C5=O)OC(/C=C(C)/C(C)C)=O)([H])[C@@]2([C@@](O5)([H])C[C@@]3([H])C(C)=C(O)C4=O)CO1)OC
Synonyms

(-)-Bruceantin; NCI165563; NSC165563
Structure Classification
- Terpenoids
- Diterpenoids
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (2)

All

Sort by:

Journal Impact Factor

Phytomedicine

Bruceantin inhibits the c-Myc/RL27A axis to suppress tumor progression in hepatocellular carcinoma. [Abstract]

2025 Dec 24:150:157740. PMID: 41477978
bioRxiv

Translation inhibition efficacy does not determine the Plasmodium berghei liver stage antiplasmodial efficacy of protein synthesis inhibitors. [Abstract]

2023 Dec 7:2023.12.07.570699. PMID: 38106175

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (182.29 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.8229 mL

9.1144 mL

18.2289 mL

5 mM

0.3646 mL

1.8229 mL

3.6458 mL

10 mM

0.1823 mL

0.9114 mL

1.8229 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (4.56 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Cuendet M, et al. Multiple myeloma regression mediated by bruceantin. Clin Cancer Res. 2004 Feb 1;10(3):1170-9. [Content Brief]

[2]. Cuendet M, et al. Antitumor activity of bruceantin: an old drug with new promise. J Nat Prod. 2004 Feb;67(2):269-72. [Content Brief]

[3]. Gillin FD, et al., Bruceantin, a potent amoebicide from a plant, Brucea antidysenterica. Antimicrob Agents Chemother. 1982 Aug;22(2):342-5. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.8229 mL	9.1144 mL	18.2289 mL	45.5722 mL
	5 mM	0.3646 mL	1.8229 mL	3.6458 mL	9.1144 mL
	10 mM	0.1823 mL	0.9114 mL	1.8229 mL	4.5572 mL
	15 mM	0.1215 mL	0.6076 mL	1.2153 mL	3.0381 mL
	20 mM	0.0911 mL	0.4557 mL	0.9114 mL	2.2786 mL
	25 mM	0.0729 mL	0.3646 mL	0.7292 mL	1.8229 mL
	30 mM	0.0608 mL	0.3038 mL	0.6076 mL	1.5191 mL
	40 mM	0.0456 mL	0.2279 mL	0.4557 mL	1.1393 mL
	50 mM	0.0365 mL	0.1823 mL	0.3646 mL	0.9114 mL
	60 mM	0.0304 mL	0.1519 mL	0.3038 mL	0.7595 mL
	80 mM	0.0228 mL	0.1139 mL	0.2279 mL	0.5697 mL
	100 mM	0.0182 mL	0.0911 mL	0.1823 mL	0.4557 mL

Bruceantin

2 Cited Publications

100 mg Angebot einholen

Subscribe to the

MedChemExpress

Newsletter

Produktname:
Art. -Nr.:
Menge:
MCE Japan Authorized Agent:

Produktname:
CAS. Nr.:
Species:
Reinheit:
Menge:

Bruceantin

MCE Bioactive Compound Library

Natural Product Library Plus

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) Bruceantin

Alle Caspase Isoform-spezifische Produkte anzeigen

Alle Parasite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Bruceantin

MCE Bioactive Compound Library

Natural Product Library Plus

High-Throughput Bioactive Compound Library

Publications Citing Use of MedChemExpress (MCE) Bruceantin

Alle Caspase Isoform-spezifische Produkte anzeigen

Alle Parasite Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Bruceantin Related Classifications

HY-N0840 Related Classifications