Cat. No.: HY-N2815

Ursolic acid acetate

(Synonyms: Acetylursolic acid; 3-Acetylursolic acid)

Customer Review

Based on 1 Customer Validation

Ursolic acid acetate (Acetylursolic acid) is a triterpenoid compound and an inhibitor of Plasmodium falciparum heat shock protein 90 (PfHsp90) with a K_D of 8.16 μM. Ursolic acid acetate is cytotoxic to KB cells, with an IC₅₀ value of 8.4 μM. Ursolic acid acetate can be used in tumor and antimalarial research.

For research use only. We do not sell to patients.

Purity: 98.0%
CAS No.: 7372-30-7
Formula: C₃₂H₅₀O₄
Molecular Weight:498.74

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Ursolic acid acetate Chemical Structure

CAS No.: 7372-30-7

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[2]^[3]

HSP90

Plasmodium

All Parasite Isoforms :

All HSP Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	GI₅₀	48 μM Compound: 17	Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 32345458]
ASPC1	IC₅₀	71 μM Compound: 2	Cytotoxicity against human AsPC1 cells after 72 hrs by MTT assay Cytotoxicity against human AsPC1 cells after 72 hrs by MTT assay	[PMID: 22959527]
BGC-823	IC₅₀	19.36 μM Compound: UA-3	Cytotoxicity against human BGC823 cells by MTT assay Cytotoxicity against human BGC823 cells by MTT assay	[PMID: 21664827]
GES1	IC₅₀	92.94 μM Compound: UA-3	Cytotoxicity against human GES-1 cells incubated for 48 hrs by MTT assay Cytotoxicity against human GES-1 cells incubated for 48 hrs by MTT assay	[PMID: 21664827]
HCT-116	GI₅₀	21 μM Compound: 17	Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 32345458]
HeLa	IC₅₀	>10 μM Compound: 2	Antiproliferative activity against human HeLa cells after 96 hrs by MTT assay Antiproliferative activity against human HeLa cells after 96 hrs by MTT assay	[PMID: 19091579]
HeLa	IC₅₀	>20 μM Compound: 2a	Cytotoxicity against human HeLa cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay Cytotoxicity against human HeLa cells assessed as inhibition of cell growth incubated for 72 hrs by CCK-8 assay	[PMID: 34688014]
HepG2	CC₅₀	>400 μM Compound: 1a	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 29326018]
HepG2	IC₅₀	37.67 μM Compound: UA-3	Cytotoxicity against human HepG2 cells incubated for 48 hrs by MTT assay Cytotoxicity against human HepG2 cells incubated for 48 hrs by MTT assay	[PMID: 21664827]
HT-29	IC₅₀	23.89 μM Compound: UA-3	Cytotoxicity against human HT-29 cells incubated for 48 hrs by MTT assay Cytotoxicity against human HT-29 cells incubated for 48 hrs by MTT assay	[PMID: 21664827]
MCF7	GI₅₀	6.5 μM Compound: 17	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 72 hrs by MTT assay	[PMID: 32345458]
NTUB1	IC₅₀	14.27 μM Compound: 12	Cytotoxicity against human NTUB1 cells after 72 hrs by MTT assay Cytotoxicity against human NTUB1 cells after 72 hrs by MTT assay	[PMID: 19758808]
Vero	CC₅₀	>400 μM Compound: 1a	Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay Cytotoxicity against African green monkey Vero cells after 72 hrs by MTT assay	[PMID: 29326018]

In Vitro

Ursolic acid acetate (1-5 mM; 4.5 h) can reduce the ATPase activity of PfHsp90 in a dose-dependent manner^[2].
Ursolic acid acetate has an IC₅₀ of 24.93 μM against the P. falciparum FcB1 strain^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 7372-30-7
Appearance Solid
Molecular Weight 498.74
Formula C₃₂H₅₀O₄
Color White to off-white
SMILES

[H][C@]12C3=CC[C@]4([C@@](CC[C@]5(C(C)([C@H](CC[C@@]54C)OC(C)=O)C)[H])([C@@]3(CC[C@]1(CC[C@H]([C@@H]2C)C)C(O)=O)C)C)[H]
Synonyms

Acetylursolic acid; 3-Acetylursolic acid
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (50.13 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.0051 mL

10.0253 mL

20.0505 mL

5 mM

0.4010 mL

2.0051 mL

4.0101 mL

10 mM

0.2005 mL

1.0025 mL

2.0051 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 1.25 mg/mL (2.51 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 1.25 mg/mL (2.51 mM); Clear solution

This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Chiang YM, et al. Cytotoxic triterpenes from the aerial roots of Ficus microcarpa. Phytochemistry. 2005 Feb;66(4):495-501. [Content Brief]

[2]. Nndwammbi AAT, et al. Ursolic acid acetate and iso-mukaadial acetate bind to Plasmodium falciparum Hsp90, abrogating its chaperone function in vitro. Naunyn Schmiedebergs Arch Pharmacol. 2024 Jul;397(7):5179-5192. [Content Brief]

[3]. Gnoatto S C B, et al. Pharmacomodulation on the 3-acetylursolic acid skeleton: Design, synthesis, and biological evaluation of novel N-{3-[4-(3-aminopropyl) piperazinyl] propyl}-3-O-acetylursolamide derivatives as antimalarial agents. Bioorganic & medicinal chemistry, 2008, 16(2): 771-782.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.0051 mL	10.0253 mL	20.0505 mL	50.1263 mL
	5 mM	0.4010 mL	2.0051 mL	4.0101 mL	10.0253 mL
	10 mM	0.2005 mL	1.0025 mL	2.0051 mL	5.0126 mL
	15 mM	0.1337 mL	0.6684 mL	1.3367 mL	3.3418 mL
	20 mM	0.1003 mL	0.5013 mL	1.0025 mL	2.5063 mL
	25 mM	0.0802 mL	0.4010 mL	0.8020 mL	2.0051 mL
	30 mM	0.0668 mL	0.3342 mL	0.6684 mL	1.6709 mL
	40 mM	0.0501 mL	0.2506 mL	0.5013 mL	1.2532 mL
	50 mM	0.0401 mL	0.2005 mL	0.4010 mL	1.0025 mL

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Ursolic acid acetate

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All Parasite Isoforms

All HSP Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Ursolic acid acetate Related Classifications

HY-N2815 Related Classifications