LJ-2P
LJ-2P is an NIR-II small-molecule fluorescent probe with fibrin/fibrinogen affinity. LJ-2P can bind coagulation-related proteins and restrict dye molecular motion, thereby enhancing local fluorescence for high-contrast imaging of bleeding and blood clots in vivo. LJ-2P can also co-precipitate with Ca2+ to form LJ-2P nanoparticles, significantly enhancing NIR-II luminescence through the precipitation-enhanced emission (PEE) mechanism. LJ-2P can be used for research on bleeding disorders and breast cancer. LJ-2P exhibits dual-modal emission properties (Ex/Em ≈740/945 nm or 740/1066 nm).
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- CAS. Nr.: 2863678-02-6
- Formel: C60H52N4O20P2S4
- Molecular Weight:1339.28
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Biologische Aktivität
Beschreibung
In Vitro
1. Stock Solution Preparation
1.1 Solvent: H2O[1].
1.2 Concentration: A high-concentration stock solution of 1-10 mM is generally recommended.
2. Working Solution Preparation
2.1 Note: Adjust the working solution concentration according to needs; prepare fresh before use.
2.2 Diluent: Serum-free medium or PBS is typically used.
2.3 Working concentration: 0.15 nM.
3. Staining Procedure
3.1 For in vitro protein samples[1]:
3.1.1 Sample types: fibrinogen, thrombin, and fibrin clots.
3.1.2 Incubate with 0.15 nM LJ-2P for 5 min.
3.1.3 To verify binding, the mixture can be placed in a 10 kDa ultrafiltration tube and centrifuged (4500 rpm, 2 min) to separate and remove the fluorescence background of unbound free dye.
4. Controls
4.1 Blank control:
LJ-2P solution is mixed with PBS buffer to adjust instrument voltage and remove background baseline.
4.1 Positive control:
Mix fibrinogen with thrombin to initiate coagulation (thrombus formation), then co-incubate with LJ-2P.
4.3 Negative control:
Use Indocyanine green (ICG) (HY-D0711) at a similar concentration and excitation wavelength for parallel incubation experiments.
5. Detection and Analysis
5.1 Instruments:
Near-infrared spectrometer, in vivo fluorescence imaging system.
5.2 Wavelength and acquisition settings:
5.2.1 Excitation: 808 nm laser.
5.2.2 Laser power: 90 mW/cm2.
5.2.3 Emission filter: 900 nm long-pass or 1000 nm long-pass.
5.3 Result analysis:
After dye staining, the fluorescence intensity of LJ-2P significantly increases 8.46-fold upon binding to fibrinogen; upon further binding to thrombus (fibrin), the fluorescence intensity surges 16.83-fold, precisely targeting the coagulation site.
LJ-2P (1-30 µM; 24 h) maintains a high cell viability and exhibits good biocompatibility in 293T cells[1].
LJ-2P can also co-precipitate with Ca2+ to form LJ-2P NPs particles, significantly enhancing NIR-II luminescence through the PEE mechanism[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:293T cell line
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Concentration:1, 5, 10, 15, 20, 25, 30 µM
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Incubation Time:24 h
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Result:Maintained high cell viability without showing obvious cytotoxicity, demonstrating good biocompatibility.
In Vivo
LJ-2P (0.25 mg/mL, 200 µL; i.v.; single administration; continuous imaging up to 24 h) enables precise localization of pulmonary hemorrhage sites and long-term stable imaging with resistance to photobleaching in the LPS (HY-D1056)-induced acute lung injury/pulmonary hemorrhage model in C57BL/6 mice[1].
LJ-2P (0.25 mg/mL, 200 µL; i.v.; single administration; continuous imaging) accurately indicates the hemorrhagic region and quantifies hemorrhage severity via fluorescence intensity in a collagenase-induced intracerebral hemorrhage model in C57BL/6 mice[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:C57BL/6 (male/female not specified; gastric hemorrhage induced by 48 h fasting followed by oral administration of 10 mL/kg anhydrous ethanol)[1]
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Dosage:0.25 mg/mL, 200 μL (imaging); 0.25 mg/kg (half-life measurement)
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Administration:i.v.; single dose; 24 h
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Result:Gradually accumulated in gastric bleeding sites, reaching peak brightness at 4 h post-injection, with fluorescence signals precisely corresponding to hemorrhagic points.
Reached a signal-to-background ratio of 10.42.
Had an imaging window of 2-8 h and a plasma half-life of approximately 2 h.
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Animal Model:C57BL/6 (male/female not specified; pulmonary hemorrhage induced by intratracheal instillation of 3 mg/kg lipopolysaccharide)[1]
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Dosage:0.25 mg/mL, 200 μL
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Administration:i.v.; single dose; 24 h
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Result:Accumulated in the lung over time, reaching peak brightness at 2 h post-injection, with fluorescence corresponding well to hemorrhage sites.
Showed minimal fluorescence intensity change after 30 min of ex vivo laser irradiation at 90 mW/cm2.
Chemical Information
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CAS. Nr. 2863678-02-6
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Molecular Weight 1339.28
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Formel C60H52N4O20P2S4
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SMILES
OP(OC1=CC2=C(C(C=CC(C3=C(OCCO4)C4=C(C5=C6C(N=S=N6)=C(C7=C8C(OCCO8)=C(C9=CC(C(CCCC(O)=O)(CCCC(O)=O)C%10=C%11C=CC(OP(O)(O)=O)=C%10)=C%11C=C9)S7)C%12=NSN=C5%12)S3)=C%13)=C%13C2(CCCC(O)=O)CCCC(O)=O)C=C1)(O)=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
LJ-2P Related Classifications
HY-D3428 Related Classifications
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