Art. -Nr.: HY-13674

Maytansine

Name: Maytansine
Brand: MedChemExpress
SKU: HY-13674
Price: 75.00 USD
Availability: InStock
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(Synonyms: NSC 153858)

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Maytansine is a highly potent microtubule-targeted compound that induces mitotic arrest and kills tumor cells at subnanomolar concentrations.

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Reinheit : 99.81%
CAS. Nr.: 35846-53-8
Formel: C₃₄H₄₆ClN₃O₁₀
Molecular Weight:692.20

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Maytansine Chemical Structure

CAS. Nr.: 35846-53-8

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Maytansine is a highly potent microtubule-targeted compound that induces mitotic arrest and kills tumor cells at subnanomolar concentrations^[1].

Cellular Effect

Cell Line	Type	Value	Description	References
A2780	IC₅₀	0.74 nM Compound: Maytansine	Antiproliferative activity against human A2780 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Antiproliferative activity against human A2780 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 38294341]
A-375	IC₅₀	0.037 nM Compound: 1	Cytotoxicity against human A375 cell line by clonogenic plating efficiency assay after 72 hrs Cytotoxicity against human A375 cell line by clonogenic plating efficiency assay after 72 hrs	[PMID: 16821799]
A-431	IC₅₀	0.04 nM Compound: 1	Cytotoxicity against human A431 cell line by clonogenic plating efficiency assay after 72 hrs Cytotoxicity against human A431 cell line by clonogenic plating efficiency assay after 72 hrs	[PMID: 16821799]
A549	IC₅₀	0.16 nM Compound: 1	Cytotoxicity against human A549 cell line by clonogenic plating efficiency assay after 72 hrs Cytotoxicity against human A549 cell line by clonogenic plating efficiency assay after 72 hrs	[PMID: 16821799]
A549	IC₅₀	0.59 nM Compound: Maytansine	Antiproliferative activity against human A549 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 38294341]
HCT-15	IC₅₀	0.75 nM Compound: 1	Cytotoxicity against human HCT15 cell line by clonogenic plating efficiency assay after 72 hrs Cytotoxicity against human HCT15 cell line by clonogenic plating efficiency assay after 72 hrs	[PMID: 16821799]
HeLa	IC₅₀	0.33 nM Compound: Maytansine	Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 38294341]
HeLa	IC₅₀	1.1 nM Compound: Maytansine	Antiproliferative activity against human HeLa cells expressing tubulin betaIII assessed as inhibition of cell growth incubated for 48 hrs by MTT assay Antiproliferative activity against human HeLa cells expressing tubulin betaIII assessed as inhibition of cell growth incubated for 48 hrs by MTT assay	[PMID: 38294341]
KB	ED₅₀	0.0000061 μg/mL Compound: 1	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 563462]
KB	ED₅₀	0.51 μg/mL Compound: Maytansine	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 7130986]
KB	IC₅₀	0.034 nM Compound: 1	Cytotoxicity against human KB cell line by clonogenic plating efficiency assay after 72 hrs Cytotoxicity against human KB cell line by clonogenic plating efficiency assay after 72 hrs	[PMID: 16821799]
MCF7	IC₅₀	0.044 nM Compound: 1	Cytotoxicity against human MCF7 cell line by clonogenic plating efficiency assay after 72 hrs Cytotoxicity against human MCF7 cell line by clonogenic plating efficiency assay after 72 hrs	[PMID: 16821799]
OVCAR-3	IC₅₀	0.1 nM Compound: 1	Cytotoxicity against human Ovcar3 cell line by clonogenic plating efficiency assay after 72 hrs Cytotoxicity against human Ovcar3 cell line by clonogenic plating efficiency assay after 72 hrs	[PMID: 16821799]
SK-BR-3	IC₅₀	0.03 nM Compound: 1	Cytotoxicity against human SK-Br-3 cell line by clonogenic plating efficiency assay after 72 hrs Cytotoxicity against human SK-Br-3 cell line by clonogenic plating efficiency assay after 72 hrs	[PMID: 16821799]
SW-620	IC₅₀	>0.1 nM Compound: 1	Cytotoxicity against human SW620 cell line by clonogenic plating efficiency assay after 72 hrs Cytotoxicity against human SW620 cell line by clonogenic plating efficiency assay after 72 hrs	[PMID: 16821799]

In Vitro

Maytansine, at 6x10^-8 M, irreversibly inhibits cell division in eggs of sea urchins and clams. Maytansine causes the disappearance of a mitotic apparatus or prevents one from forming if added at early stages. Maytansine inhibits in vitro polymerization of tubulin^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .

Chemical Information

CAS. Nr. 35846-53-8
Appearance Solid
Molecular Weight 692.20
Formel C₃₄H₄₆ClN₃O₁₀
Color White to off-white
SMILES

C[C@]12[C@@]([C@@H]([C@]3([H])C[C@]([C@@H](/C=C/C=C(CC4=CC(N(C(C[C@@H]2OC([C@H](C)N(C)C(C)=O)=O)=O)C)=C(C(OC)=C4)Cl)\C)OC)(NC(O3)=O)O)C)([H])O1
Synonyms

NSC 153858
Structure Classification
- Alkaloids
- Other Alkaloids
Initial Source
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (144.47 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.4447 mL

7.2233 mL

14.4467 mL

5 mM

0.2889 mL

1.4447 mL

2.8893 mL

10 mM

0.1445 mL

0.7223 mL

1.4447 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (3.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (3.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (3.61 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Lopus M, et al. Maytansine and cellular metabolites of antibody-maytansinoid conjugates strongly suppress microtubule dynamics by binding to microtubules. Mol Cancer Ther. 2010;9(10):2689-2699. [Content Brief]

[2]. Remillard S, et al. Antimitotic activity of the potent tumor inhibitor maytansine. Science. 1975;189(4207):1002-1005. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.4447 mL	7.2233 mL	14.4467 mL	36.1167 mL
	5 mM	0.2889 mL	1.4447 mL	2.8893 mL	7.2233 mL
	10 mM	0.1445 mL	0.7223 mL	1.4447 mL	3.6117 mL
	15 mM	0.0963 mL	0.4816 mL	0.9631 mL	2.4078 mL
	20 mM	0.0722 mL	0.3612 mL	0.7223 mL	1.8058 mL
	25 mM	0.0578 mL	0.2889 mL	0.5779 mL	1.4447 mL
	30 mM	0.0482 mL	0.2408 mL	0.4816 mL	1.2039 mL
	40 mM	0.0361 mL	0.1806 mL	0.3612 mL	0.9029 mL
	50 mM	0.0289 mL	0.1445 mL	0.2889 mL	0.7223 mL
	60 mM	0.0241 mL	0.1204 mL	0.2408 mL	0.6019 mL
	80 mM	0.0181 mL	0.0903 mL	0.1806 mL	0.4515 mL
	100 mM	0.0144 mL	0.0722 mL	0.1445 mL	0.3612 mL

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Maytansine