MLS-0053105
Based on 1 Customer Validation
MLS-0053105, a chloromaleimide, is a selective BFL-1 inhibitor with an IC50 of 0.4 µM for Bfl-1/F-Bid. MLS-0053105 shows over 10 times less potent inhibition of Bcl-W, Bcl-2, and Bcl-XL and no activity against Bcl-B and Mcl-1.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 98.89%
- CAS. Nr.: 370572-36-4
- Formel: C15H14Cl3N3O2
- Molecular Weight:374.65
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Speicherung:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
IC50 & Target
[1]|
Bfl-1/F-Bid 0.4 μM (IC50) |
Bcl-W/F-Bim 12.5 μM (IC50) |
Bcl-2/F-Bim 12.5 μM (IC50) |
Bcl-XL/F-Bim 50 μM (IC50) |
Chemical Information
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CAS. Nr. 370572-36-4
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Appearance Solid
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Molecular Weight 374.65
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Formel C15H14Cl3N3O2
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Color Yellow to orange
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SMILES
O=C1C(Cl)=C(N2CCN(CC2)C)C(N1C3=CC=C(C(Cl)=C3)Cl)=O
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (266.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protokoll
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Reinheit & Dokumentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 2.6692 mL | 13.3458 mL | 26.6916 mL | 66.7289 mL |
| 5 mM | 0.5338 mL | 2.6692 mL | 5.3383 mL | 13.3458 mL | |
| 10 mM | 0.2669 mL | 1.3346 mL | 2.6692 mL | 6.6729 mL | |
| 15 mM | 0.1779 mL | 0.8897 mL | 1.7794 mL | 4.4486 mL | |
| 20 mM | 0.1335 mL | 0.6673 mL | 1.3346 mL | 3.3364 mL | |
| 25 mM | 0.1068 mL | 0.5338 mL | 1.0677 mL | 2.6692 mL | |
| 30 mM | 0.0890 mL | 0.4449 mL | 0.8897 mL | 2.2243 mL | |
| 40 mM | 0.0667 mL | 0.3336 mL | 0.6673 mL | 1.6682 mL | |
| 50 mM | 0.0534 mL | 0.2669 mL | 0.5338 mL | 1.3346 mL | |
| 60 mM | 0.0445 mL | 0.2224 mL | 0.4449 mL | 1.1121 mL | |
| 80 mM | 0.0334 mL | 0.1668 mL | 0.3336 mL | 0.8341 mL | |
| 100 mM | 0.0267 mL | 0.1335 mL | 0.2669 mL | 0.6673 mL |
MLS-0053105 Related Classifications
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