Paridiprubart (WT IgG1)
Based on 1 Customer Validation
Paridiprubart (WT IgG1) (NI-0101) is a humanised anti-TLR4 monoclonal antibody. Paridiprubart (WT IgG1) inhibits the production of pro-inflammatory cytokines by monocytes from rheumatoid arthritis specimen induced by rheumatoid arthritis synovial fluid (RASF). Paridiprubart (WT IgG1) can be used for the study of rheumatoid arthritis.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : ≥95.0%
- CAS. Nr.: 2641646-59-3
- Molecular Weight:145.16 kDa
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biologische Aktivität
Beschreibung
Isotype
Human IgG1 kappa
Recommend Isotype Controls
Species Reactivity
Human
IC50 & Target
TLR4[1]
In Vitro
Paridiprubart (WT IgG1) (20 μg/mL, pre-treated for 30 minutes) significantly inhibits the production of pro-inflammatory cytokines by monocytes from rheumatoid arthritis specimen induced by rheumatoid arthritis synovial fluid (RASF)[1].
Paridiprubart (WT IgG1) inhibits the production of TNF-α by macrophages stimulated by citrullinated fibrinogen immune complexes (cFb-IC) through an Fc-dependent mechanism[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Monocytes from rheumatoid arthritis specimen
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Concentration:20 μg/mL
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Incubation Time:pre-treated for 30 minutes
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Result:Reduced the production of pro-inflammatory cytokines such as IL-6, TNFα, IL-1β and IL-8.
Clinical Trial
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Format
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Human IgG1 kappa
Anwendung
ELISA, FACS, Functional assay
Verified Bioactivity
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Flow cytometric analysis of 1X106 Jurkat cells with Paridiprubart (HY-145645, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Chemical Information
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CAS. Nr. 2641646-59-3
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Appearance Liquid
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Molecular Weight 145.16 kDa
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Color Colorless to light yellow
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SMILES
[Paridiprubart (WT IgG1)]
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Synonyms
NI-0101
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Versand
Shipping with dry ice.
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
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Data Sheet (269 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)