Art. -Nr.: HY-10496

SC75741

35 Cited Publications

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Based on 35 publication(s) in Google Scholar

SC75741 is a broad and efficient NF-κB inhibitor with an IC₅₀ of 200 nM for p65. SC75741 blocks influenza viruses (IV) replication. SC75741 impairs DNA binding of the NF-κB subunit p65, resulting in reduced expression of cytokines, chemokines, and pro-apoptotic factors. SC75741 subsequently inhibits caspase activation and blocks caspase-mediated nuclear export of viral ribonucleoproteins.

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Reinheit: 99.24%
CAS. Nr.: 913822-46-5
Formel: C₂₉H₂₃N₇O₂S₂
Molecular Weight:565.67

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

SC75741 Chemical Structure

CAS. Nr.: 913822-46-5

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (35)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

SC75741 is a broad and efficient NF-κB inhibitor with an IC₅₀ of 200 nM for p65^[1]. SC75741 blocks influenza viruses (IV) replication. SC75741 impairs DNA binding of the NF-κB subunit p65, resulting in reduced expression of cytokines, chemokines, and pro-apoptotic factors. SC75741 subsequently inhibits caspase activation and blocks caspase-mediated nuclear export of viral ribonucleoproteins^[2].

IC₅₀ & Target^[1,2]

p65

200 nM (IC₅₀)

Alle NF-κB Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	EC₅₀	0.2 μM Compound: 13	Inhibition of NF-kappaB in A549 cells after 5 hrs by reporter gene assay Inhibition of NF-kappaB in A549 cells after 5 hrs by reporter gene assay	[PMID: 17869512]
PBMC	IC₅₀	2.2 μM Compound: 13	Antiproliferative activity against human PBMC Antiproliferative activity against human PBMC	[PMID: 17869512]
THP1-Dual	IC₅₀	3958 nM Compound: SC75741	Inhibition of LPS induced ISG activation in human THP1-Dual cells pretreated with LPS-B5 for 3 hrs followed by compound addition incubated for 24 hrs Inhibition of LPS induced ISG activation in human THP1-Dual cells pretreated with LPS-B5 for 3 hrs followed by compound addition incubated for 24 hrs	[PMID: 36053746]
THP1-Dual	IC₅₀	5975 nM Compound: SC75741	Inhibition of LPS induced NF kappa B activation in human THP1-Dual cells stimulated with LPS-B5 for 3 hrs followed by compound addition incubated for 24 hrs Inhibition of LPS induced NF kappa B activation in human THP1-Dual cells stimulated with LPS-B5 for 3 hrs followed by compound addition incubated for 24 hrs	[PMID: 36053746]

In Vitro

SC75741 (5 μM; 24-96 hours) inhibits long-term A549 cells proliferation^[2].
SC75741 (1-10 μM; 5.5-65 hours) reduces A549 cells viability in a concentration-dependent manner indicating a cytostatic effect for A549 cells within a time frame of about 50 and 65 hours^[2].
SC75741 (5 μM; 24 hours) strongly inhibits cleavage of the effector caspase 3 induced upon H7N7-infection^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[2]

Cell Line:

A549 cells
Concentration:

5 μM
Incubation Time:

24, 48, 72 and 96 hours
Result:

Inhibited long-term cell proliferation

Cell Viability Assay^[2]

Cell Line:

A549 cells
Concentration:

1, 2, 5 or 10 μM
Incubation Time:

5.5, 29, 50, 65 hours
Result:

Reduced cells viability in a concentration-dependent manner.

Western Blot Analysis^[2]

Cell Line:

MDCK cells
Concentration:

5 μM
Incubation Time:

24 hours
Result:

Inhibited cleavage of the effector caspase 3 induced upon H7N7-infection.

In Vivo

SC75741 (intraperitoneal injection; 15 mg/kg; for 2 days) leads to a reduced propagation of the H5N1 virus mRNA by 90% in the lungs of infected mice^[2].
The plasma-levels of SC74751 (intravenously of 5 mg/kg and intraperitoneally of 15 mg/kg; for 3.5 and 6 hours) after i.v. administration decreases mono-exponentially and half-life is roughly 40 min. After i.p. administration, elimination of SC75741 seems to be limited by a slow uptake from the peritoneum and a half-life of 55 min is observed^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Inbred female C57BL/6 mice at the age of 6-8 weeks^[2]
Dosage:

15 mg/kg
Administration:

Intraperitoneal injection; for 2 days
Result:

Reduced the amount of viral mRNA by 90%.

Animal Model:

Inbred female C57BL/6 mice at the age of 6-8 weeks^[1]
Dosage:

5 mg/kg or 15 mg/kg
Administration:

Intravenously of 5 mg/kg and intraperitoneally of 15 mg/kg; 3.5 and 6 hours
Result:

Half-life was roughly 40 min and 55 min for i.v. and i.p. administration, respectively.

Chemical Information

CAS. Nr. 913822-46-5
Appearance Solid
Molecular Weight 565.67
Formel C₂₉H₂₃N₇O₂S₂
Color Light yellow to brown
SMILES

O=C(NC1=NC(C=CC(C(C2=CC=CC=C2)=O)=C3)=C3N1)C4=CSC(C(CC5)CCN5C6=NC=NC7=C6SC=C7)=N4
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (35)

All WBRT-PCRCell Imaging/StainingCell Proliferation/Viability Assay

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Journal Impact Factor

Sci Bull

Initial cardioplegic flush with crystalloid cardioplegia improves donor heart preservation and function via NR4A3 upregulation and metabolic reprogramming. [Abstract]

2025 May 30;70(10):1673-1690. PMID: 40190001

SC75741 purchased from MedChemExpress. Usage Cited in: Sci Bull. 2025 May 30;70(10):1673-1690. [Abstract]

SC75741 (5 μmol/L). Western blot analysis showed that SC75741 treatment significantly downregulated the expression of p-p65 (Ser529).

SC75741 purchased from MedChemExpress. Usage Cited in: Sci Bull. 2025 May 30;70(10):1673-1690. [Abstract]

SC75741 (5 μmol/L). The fluorescence staining confirmed NFκB inhibition led to less myocardial apoptosis and ROS production after 8-hours SCS.
J Clin Invest

Symbiotic exclusivity between CLOCK and TFPI2 drives stemness and immunosuppression in glioblastoma models. [Abstract]

2026 Mar 17:e199056. PMID: 41842961
J Clin Invest

Dual targeting macrophages and microglia is a therapeutic vulnerability in models of PTEN-deficient glioblastoma. [Abstract]

2024 Oct 1;134(22):e178628. PMID: 39352749

SC75741 purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2024 Oct 1;134(22):e178628. [Abstract]

Relative mRNA expression of OLFML3 in PTEN-KO SF763 cells expressing shC and shLOX treated with or without P65 inhibitor (P65i) SC75741 (5 μM, 24h).

SC75741 purchased from MedChemExpress. Usage Cited in: J Clin Invest. 2024 Oct 1;134(22):e178628. [Abstract]

Immunoblots for OLFML3 in lysates of PTEN-WT SF763 cells expressing shC and shPATZ1 treated with or without P65i SC75741 (5 μM, 24h).
Cell Rep Med

A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML. [Abstract]

2024 May 29:101592. PMID: 38843841

SC75741 purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2024 May 29:101592. [Abstract]

MV4-11 cells stimulated in LDM supplied with different reagents (PA, 20 μM; OxLDL, 25 μg/mL; SC75741, 5 μM) for 3 days were further used for T cell proliferation assay (n = 3).

SC75741 purchased from MedChemExpress. Usage Cited in: Cell Rep Med. 2024 May 29:101592. [Abstract]

Different kinds of MV4-11 cells were used for T cell proliferation assay (n = 3). SC75741 (5 μM) was added.
Cell Mol Biol Lett

Immunodynamic axis of fibroblast-driven neutrophil infiltration in acute pancreatitis: NF-κB-HIF-1α-CXCL1. [Abstract]

2025 May 7;30(1):57. PMID: 40335899
Diabetologia

MAP4K4 aggravates microvascular anomalies in diabetic retinopathy in a YTHDF2-dependent manner. [Abstract]

2025 Jun;68(6):1335-1351. PMID: 40072537
Cell Commun Signal

Senescent cells promote viral infection-associated inflammation and tissue damage through a robust NF-κB pathway. [Abstract]

2025 Oct 28;23(1):464. PMID: 41152908
Phytomedicine

Network-guided identification and in vitro/in vivo validation of Withaferin A as an inhibitor of nuclear factor kappa B for spinal cord injury repair. [Abstract]

2026 Aug:158:158380. PMID: 42275873
Phytomedicine

Shen-Shuai-II-Recipe inhibits tubular inflammation by PPARα-mediated fatty acid oxidation to attenuate fibroblast activation in fibrotic kidneys. [Abstract]

2024 Apr:126:155450. PMID: 38368794
Free Radic Biol Med

Cannabidiol mitigates radiation-induced intestine ferroptosis via facilitating the heterodimerization of RUNX3 with CBFβ thereby promoting transactivation of GPX4. [Abstract]

2024 Jun 1:S0891-5849(24)00506-9. PMID: 38830513
Cell Rep

Neuropeptide adrenomedullin remodels stemness and macrophage dynamics in glioblastoma. [Abstract]

2025 Sep 24;44(10):116342. PMID: 41004340
Oncogenesis

Transgelin promotes lung cancer progression via activation of cancer-associated fibroblasts with enhanced IL-6 release. [Abstract]

2023 Mar 29;12(1):18. PMID: 36990991
EMBO Rep

PEBP1 suppresses HIV transcription and induces latency by inactivating MAPK/NF-κB signaling. [Abstract]

2020 Nov 5;21(11):e49305. PMID: 32924251
Int J Mol Med

Platelet activation stimulates macrophages to enhance ulcerative colitis through PF4/CXCR3 signaling. [Abstract]

2025 May;55(5):78. PMID: 40084691
Inflammation

REGγ-mediated Barrier Disruption and NF-κB Activation Aggravates Intestinal Inflammation in Necrotizing Enterocolitis. [Abstract]

2024 Dec 10. PMID: 39653983
CNS Neurosci Ther

Regulation of temozolomide resistance in glioma cells via the RIP2/NF-κB/MGMT pathway. [Abstract]

2021 May;27(5):552-563. PMID: 33460245
PLoS Pathog

Candida albicans translocation through the intestinal epithelial barrier is promoted by fungal zinc acquisition and limited by NFκB-mediated barrier protection. [Abstract]

2024 Mar 1;20(3):e1012031. PMID: 38427950
Int Immunopharmacol

Α-Hederin alleviates psoriasiform skin inflammation by inhibiting NF-κB p65/CXCL2 Axis. [Abstract]

2026 May 1:176:116462. PMID: 41797086
Am J Physiol Cell Physiol

Immunoproteasome subunit PSMB8 promotes skeletal muscle regeneration by regulating macrophage phenotyping switch in mice. [Abstract]

2025 Apr 16. PMID: 40241316
Int Immunopharmacol

Long non-coding RNA NRIR inhibits osteogenesis in peri-implantitis by promoting NLRP3 inflammasome-mediated macrophage pyroptosis. [Abstract]

2025 Jan 27:148:114180. PMID: 39874847
Int Immunopharmacol

Mechanism of S100A9-mediated astrocyte activation via TLR4/NF-κB in Parkinson's disease. [Abstract]

2024 Dec 25:146:113938. PMID: 39724736
Eur J Pharmacol

Daidzein ameliorates LPS-induced hepatocyte injury by inhibiting inflammation and oxidative stress. [Abstract]

2020 Oct 15;885:173399. PMID: 32712091
Mol Neurobiol

Transcriptome Analysis Reveals Dynamic Microglial-Induced A1 Astrocyte Reactivity via C3/C3aR/NF-κB Signaling After Ischemic Stroke. [Abstract]

2024 Dec;61(12):10246-10270. PMID: 38713438
J Cell Mol Med

HIF-1α is necessary for activation and tumour-promotion effect of cancer-associated fibroblasts in lung cancer. [Abstract]

2021 Jun;25(12):5457-5469. PMID: 33943003
J Cell Mol Med

S100A9 induces nucleus pulposus cell degeneration through activation of the NF-κB signaling pathway. [Abstract]

2021 May;25(10):4709-4720. PMID: 33734570
Poultry Sci

SC75741 antagonizes vesicular stomatitis virus, duck Tembusu virus, and duck plague virus infection in duck cells through promoting innate immune responses. [Abstract]

2021 May;100(5):101085. PMID: 33799115
Sci Rep

Targeted deletion of c-kit in TECs attenuates UUO-induced renal fibrosis through NF-κB pathway inhibition. [Abstract]

2026 Mar 12;16(1):13227. PMID: 41820550
Mol Biol Rep

Porphyromonas gingivalis-derived lipopolysaccharide confers chemotherapy resistance and migratory ability on oral cancer cells by activating toll-like receptor 4 signaling pathway. [Abstract]

2026 Mar 27;53(1):551. PMID: 41894086
J Biochem Mol Toxicol

Brucine Inhibits Gastric Cancer via Activation of Ferroptosis Through Regulating the NF-κB Signaling Pathway. [Abstract]

2025 Sep;39(9):e70479. PMID: 40919637
Cancer Manag Res

Protocadherin 1 (PCDH1) Promotes Pancreatic Cancer Metastasis by Regulating Epithelial-Mesenchymal Transition (EMT) Progression Through the NF-κB Pathway. [Abstract]

2026 Apr 11:18:592744. PMID: 42005443
Immunol Invest

Mechanistic Insights into HOTAIR-Driven ADAM17/NF-Κb Activation and Endothelial Dysfunction in LPS-Challenged HUVECs. [Abstract]

2025 May 14:1-27. PMID: 40366882
Cell Div

Linalool targets NR3C2 to inhibit NF-κB-mediated gastric cancer progression. [Abstract]

2026 May 18;21(1):15. PMID: 42151974
McGill University

2026.
Zoonoses

2023 Feb 11.
Research Square Preprint

2021 Dec.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 83.33 mg/mL (147.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.7678 mL

8.8391 mL

17.6782 mL

5 mM

0.3536 mL

1.7678 mL

3.5356 mL

10 mM

0.1768 mL

0.8839 mL

1.7678 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (3.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.08 mg/mL (3.68 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (3.68 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Haasbach E, et al. The NF-kappaB inhibitor SC75741 protects mice against highly pathogenic avian influenza A virus. Antiviral Res. 2013 Sep;99(3):336-44. [Content Brief]

[2]. Ehrhardt C, et al. The NF-κB inhibitor SC75741 efficiently blocks influenza virus propagation and confers a high barrier for development of viral resistance. Cell Microbiol. 2013 Jul;15(7):1198-211. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.7678 mL	8.8391 mL	17.6782 mL	44.1954 mL
	5 mM	0.3536 mL	1.7678 mL	3.5356 mL	8.8391 mL
	10 mM	0.1768 mL	0.8839 mL	1.7678 mL	4.4195 mL
	15 mM	0.1179 mL	0.5893 mL	1.1785 mL	2.9464 mL
	20 mM	0.0884 mL	0.4420 mL	0.8839 mL	2.2098 mL
	25 mM	0.0707 mL	0.3536 mL	0.7071 mL	1.7678 mL
	30 mM	0.0589 mL	0.2946 mL	0.5893 mL	1.4732 mL
	40 mM	0.0442 mL	0.2210 mL	0.4420 mL	1.1049 mL
	50 mM	0.0354 mL	0.1768 mL	0.3536 mL	0.8839 mL
	60 mM	0.0295 mL	0.1473 mL	0.2946 mL	0.7366 mL
	80 mM	0.0221 mL	0.1105 mL	0.2210 mL	0.5524 mL
	100 mM	0.0177 mL	0.0884 mL	0.1768 mL	0.4420 mL

Keywords

SC75741

35 Cited Publications

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SC75741

Anti-Infection Compound Library

Immunology/Inflammation Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Anti-Cancer Compound Library

Antiviral Compound Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Oxygen Sensing Compound Library

Pyroptosis Compound Library

Anti-Breast Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Obesity Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Antidepressant Compound Library

Anti-Prostate Cancer Compound Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Pain-Related Compound Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

Anti-Fibrosis Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) SC75741

Alle NF-κB Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (35)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

SC75741 Related Classifications

HY-10496 Related Classifications