country

Change Language

EN - English
FR - Français
DE - Deutsch
ES - Español
JA - 日本語
KO - 한국어

Country or Region:

United States

Product Name	Size / Unit Price	Qty

Total: USD 0.00 Checkout

Art. -Nr.: HY-187596

Copy Link Print Information

Sjc 13

Sjc 13 is an inhibitor of E-selectin and VCAM-1, with IC₅₀ values of 150 μg/mL and 115 μg/mL, respectively. Sjc 13 prevents neutrophil migration and adhesion by selectively inhibiting the expression and mRNA synthesis of E-selectin and VCAM-1 in LPS-stimulated endothelial cells, thereby protecting against LPS-induced lethal shock. Sjc 13 is applicable to the study of septic shock.

Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.

CAS. Nr.: 133669-72-4
Formel: C₁₇H₁₈N₃NaO₄
Molecular Weight:351.33

Speicherung:

Please store the product under the recommended conditions in the Certificate of Analysis.

Sjc 13 Chemical Structure

CAS. Nr.: 133669-72-4

Größe

Preis

Verfügbarkeit

Menge

Amount: USD 0.00

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Reinheit & Dokumentation
Verweise
Calculators
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

^[2]

E-selectin

150 μg/mL (IC₅₀)

VCAM-1

115 μg/mL (IC₅₀)

Alle DNA/RNA Synthesis Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
HUVEC	IC50	150 μg/mL	Inhibition of LPS-induced E-selectin expression on human umbilical vein endothelial cells (HUVEC) assessed by cellular enzyme-linked immunosorbent assay (cell-ELISA) following 1 h pretreatment with Sjc 13 prior to 4 h LPS stimulation. Inhibition of LPS-induced E-selectin expression on human umbilical vein endothelial cells (HUVEC) assessed by cellular enzyme-linked immunosorbent assay (cell-ELISA) following 1 h pretreatment with Sjc 13 prior to 4 h LPS stimulation.	8737744
HUVEC	IC50	760 μg/mL	Inhibition of LPS-induced ICAM-1 expression on human umbilical vein endothelial cells (HUVEC) assessed by cellular enzyme-linked immunosorbent assay (cell-ELISA) following 1 h pretreatment with Sjc 13 prior to 24 h LPS stimulation. Inhibition of LPS-induced ICAM-1 expression on human umbilical vein endothelial cells (HUVEC) assessed by cellular enzyme-linked immunosorbent assay (cell-ELISA) following 1 h pretreatment with Sjc 13 prior to 24 h LPS stimulation.	8737744
HUVEC	IC50	115 μg/mL	Inhibition of LPS-induced VCAM-1 expression on human umbilical vein endothelial cells (HUVEC) assessed by cellular enzyme-linked immunosorbent assay (cell-ELISA) following 1 h pretreatment with Sjc 13 prior to 24 h LPS stimulation. Inhibition of LPS-induced VCAM-1 expression on human umbilical vein endothelial cells (HUVEC) assessed by cellular enzyme-linked immunosorbent assay (cell-ELISA) following 1 h pretreatment with Sjc 13 prior to 24 h LPS stimulation.	8737744

In Vitro

Sjc 13 (1 mg/mL; 1 h pre-incubation, 4 h/24 h Lipopolysaccharides, from E. coli O111:B4 (LPS) (HY-D1056A1) stimulation) completely inhibits the adhesion of HL60 cells to LPS-stimulated HUVECs at 4 h after stimulation, but exerts no effect on adhesion at 24 h after stimulation^[2].
Sjc 13 (1 mg/mL; 1 h pre-incubation, 4 h/24 h LPS stimulation) inhibits LPS-induced expression of E-selectin (expression reduced by 95%) and VCAM-1 (expression reduced by 94%) on human umbilical vein endothelial cells (HUVEC), but does not affect ICAM-1 expression and has no impact on basal adhesion molecule levels^[2].
Sjc 13 (62.5-1000 μg/mL; 1 h pre-incubation, 4 h/24 h LPS stimulation) exerts a stronger inhibitory effect on LPS-induced expression of E-selectin (IC₅₀ = 150 μg/mL) and VCAM-1 (IC₅₀ = 115 μg/mL) in human umbilical vein endothelial cells (HUVEC) than on LPS-induced ICAM-1 expression (IC₅₀ = 760 μg/mL), and exhibits concentration-dependent activity in the range of 62.5 to 1000 μg/mL^[2].
Sjc 13 (1 mg/mL; 1 h pre-incubation, 4 h LPS stimulation) inhibits LPS-induced mRNA expression of E-selectin and VCAM-1 in human umbilical vein endothelial cells (HUVEC), but does not affect LPS-induced ICAM-1 mRNA levels^[2].
Sjc 13 inhibits the adhesion of HL60 cells to LPS-stimulated HUVECs, and selectively suppresses the expression and mRNA synthesis of E-selectin and VCAM-1 in LPS-stimulated HUVECs when administered 1-2 h after LPS stimulation; its activity is lost if it is added 2 h after LPS stimulation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[2]

Cell Line:

human umbilical vein endothelial cells (HUVEC)
Concentration:

1 mg/mL
Incubation Time:

1 h pre-incubation
Result:

Did not show any cytotoxicity to HUVEC.

In Vivo

SJC13 (1-10 mg/kg; i.v.; single dose) dose-dependently protects against LPS-induced septic shock in BALB/c mice, with 10 mg/kg providing robust prophylactic efficacy and efficacy when administered up to 1 hour after LPS challenge, and also reduces lung neutrophil emigration at this dose^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c (7-week-old female, specific pathogen-free, septic shock model via intravenous LD₁₀₀ LPS injection)^[1]
Dosage:

1 mg/kg (pretreatment); 3 mg/kg (pretreatment); 10 mg/kg (pretreatment; 5 min post-LPS; 30 min post-LPS; 1 h post-LPS; 2 h post-LPS)
Administration:

i.v.; single dose
Result:

Provided partial protection against LPS-induced lethality at 1 mg/kg when administered 5 min prior to LPS.
Prevented LPS-induced lethality at 3 mg/kg and 10 mg/kg when administered 5 min prior to LPS.
Protected against LPS-induced lethality at 10 mg/kg when administered 5 min, 30 min, or 1 h after LPS.
Showed little protection against LPS-induced lethality at 10 mg/kg when administered 2 h after LPS.
Reduced lung MPO activity to 0.207 OD at 450 nm at 10 mg/kg when administered 5 min prior to LPS.
Greatly reduced neutrophil margination, aggregation in microvasculature, and intra-alveolar infiltration in lung tissue at 10 mg/kg when administered 5 min prior to LPS.
Did not significantly reduce lung MPO activity at 1 mg/kg when administered 5 min prior to LPS.

Chemical Information

CAS. Nr. 133669-72-4
Molecular Weight 351.33
Formel C₁₇H₁₈N₃NaO₄
SMILES

N#C/C(C(O[Na])=O)=C\C1=C(OC(C)C)N=C2C(OC(C)C)=CC=CN21
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

Please store the product under the recommended conditions in the Certificate of Analysis.

Reinheit & Dokumentation

Handling Instructions (2659 KB)

Verweise

[1]. Sakai A, et al. Protection against septic shock in mice with SJC13, an azaindolidine derivative that is a cell adhesion molecule inhibitor. Inflammation research : official journal of the European Histamine Research Society ... [et al.]. 1996 Sep;45(9):448-51.

[2]. Sakai A, et al. Inhibition of endothelial cell adhesion molecule expression with SJC13, an azaindolidine derivative, in vitro. Inflammation research : official journal of the European Histamine Research Society ... [et al.]. 1996 May;45(5):224-9.

Calculators

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Bioz

Keywords

MOQ

Minimum order quantity

100 mg

Angebot einholen Auf Lager

Other size

Angebot einholen